Preparation method of high-purity mannan oligosaccharide
A high-purity mannan oligosaccharide technology, applied in the preparation of mannan oligosaccharides, ceramic membrane separation and preparation of high-purity mannan oligosaccharides, can solve the problem of increasing enzymatic hydrolysis raw material induction and subsequent application instructions, affecting product yield and production costs, the process has not been simplified and improved, etc., to achieve the effect of reducing cleaning frequency, good anti-fouling ability, and high operating pressure
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Embodiment 1
[0046] A preparation method of high-purity mannooligosaccharides, comprising the steps of:
[0047] (1) Manno-oligosaccharide mixture produced by enzymatic hydrolysis and transformation: use Aspergillus niger to ferment to produce highly active neutral β-mannanase, the preservation number is: CCTCC NO.M2012554, the enzyme activity is as high as 5000-8000μmol / ml The solution is purified and stored at 0 or 1 or 2 or 3 or 4 or 5°C for use. Add 2 tons of water to a 5-ton enzymatic hydrolysis tank, heat it to 55°C with steam, add 4L of neutral β-mannanase enzyme solution, stir for 2 or 3 minutes to activate the enzyme solution, and slowly add the viscosity to 20000mpa.s Konjac fine powder 400kg, add in 1 hour. Keep the enzymolysis temperature at 53 or 54 or 55 or 56 or 57°C, pH6.5, enzymolysis time or 2 or 3 or 4 hours, stop enzymolysis when the viscosity of the mannan-oligosaccharide mixture is 40mpa.s, and obtain the oligosaccharide mixture , wherein the biotransformation rate ...
Embodiment 2
[0062] A preparation method of high-purity mannooligosaccharides, comprising the steps of:
[0063] (1) Manno-oligosaccharide mixture produced by enzymatic hydrolysis and conversion: using Aspergillus niger to ferment to produce a highly active neutral β-mannanase, the preservation number is: CCTCC NO.M2012554, the enzyme activity is as high as 4500 μmol / ml, and the enzyme solution is passed through The purification process is stored at 0-5°C for later use. Add 2.5 tons of water into a 5-ton enzymatic hydrolysis tank, heat it to 54°C with steam, add 4.5L of neutral β-mannanase enzyme solution, stir for 2 to 3 minutes to activate the enzyme solution, and slowly add the viscosity to 20000mpa. s konjac powder 550kg, add in 1 hour. Keep the enzymolysis temperature at 53-56°C, pH6.5, enzymolysis time 3.5 hours, measure the viscosity of the manno-oligosaccharide mixture to 60mpa.s, stop the enzymolysis, and obtain the oligosaccharide mixture, in which the glucomannan in konjac flou...
Embodiment 3
[0079] A preparation method of high-purity mannooligosaccharides, comprising the steps of:
[0080] (1) Manno-oligosaccharide mixture produced by enzymatic hydrolysis and conversion: using Aspergillus niger to ferment to produce a highly active neutral β-mannanase, the preservation number is: CCTCC NO.M2012554, the enzyme activity is as high as 4500 μmol / ml, and the enzyme solution is passed through The purification process is stored at 0-5°C for later use. Add 1.5 tons of water into a 5-ton enzymatic hydrolysis tank, heat it to 53°C with steam, add 2.5L of neutral β-mannanase enzyme solution, stir for 3 minutes to activate the enzyme solution, and slowly add Guar gum 255kg, added in 1 hour. Keep the enzymolysis temperature at 53-57°C, pH 6.0, and enzymolysis time for 4.5 hours to measure the viscosity of the mannan-oligosaccharide mixture to 70mpa. The biotransformation rate is 96%.
[0081] (2) Centrifugal separation:
[0082]Use a horizontal screw centrifuge LW250-1000 ...
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