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Preparation method of compound effervescent tablets for treating chicken infectious bursal disease

A technology for infectious and bursal disease of chickens, which is applied in the directions of medical preparations, pharmaceutical formulations, antiviral agents, etc. containing active ingredients, which can solve the inconvenience of storage and transportation for users, it is difficult to ensure accurate measurement of animals, and reduce the risk of living. The number of bacteria and other issues can improve the overall immune effect, enhance the immunity of chickens, and reduce stress.

Active Publication Date: 2015-03-04
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this feeding method is simple and convenient, since the probiotics are mostly lactic acid bacteria, their vitality is relatively fragile, and mixing them in feed will significantly reduce the number of viable bacteria
In addition, due to co-feeding with the feed, it is difficult to ensure the accurate measurement of animal absorption, thus affecting the curative effect
In addition, the freeze-dried product must be kept in a "cold chain" environment at a lower temperature during the entire storage and transportation process, which brings a lot of inconvenience to the storage and transportation of the product and the use of users.

Method used

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  • Preparation method of compound effervescent tablets for treating chicken infectious bursal disease
  • Preparation method of compound effervescent tablets for treating chicken infectious bursal disease
  • Preparation method of compound effervescent tablets for treating chicken infectious bursal disease

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: use DF-1 cell line to cultivate chicken infectious bursal virus to prepare chicken infectious bursal virus suspension

[0037] (1) Passage and culture of cells for seedling production: take chicken embryo fibroblast passage cell line DF-1 cell line, digest and passage with trypsin cell dispersion liquid, and continue to culture with cell growth liquid. When a good monolayer is formed, it is used to continue passaging or inoculation with virus;

[0038] (2) Propagation of cytotoxic seeds: Take the infectious bursal virus B87 strain (purchased from the China Veterinary Drug Administration) virus seeds for production, and inoculate 1% of the maintenance fluid volume in chickens that have grown into a good monolayer. Place embryonic fibroblast passage cells in the maintenance medium at 37-38°C to continue culturing, and harvest the cell fluid when the cell lesion rate reaches 75% or more;

[0039] (3) Propagation of venom for seedling production: take the abo...

Embodiment 2

[0041] Embodiment 2: the preparation of probiotic mixed bacteria liquid

[0042] (1) Cultivation of strains

[0043] Culture of lactic acid bacteria

[0044] Preheat the MRS liquid medium to 37°C, and inoculate the lactic acid bacteria seed liquid at 2% inoculum, let it stand at 37°C for 18-24 hours, harvest the bacterial liquid and store it at -4°C for later use;

[0045] Culture of Bacillus

[0046] Preheat the Bacillus culture medium to 37°C, at the same time inoculate the Bacillus seed solution with 1% inoculation amount, let it stand at 37°C for 18-24 hours, harvest the bacteria solution and store it at 4°C for later use;

[0047] Described lactic acid bacteria are: Bifidobacterium bifidum ( Bifidobacterium bifidum ), Lactobacillus ( Lactobacillus lactis ), Lactobacillus Brucella ( Lactobacillus buchneri ), Bifidobacterium longum ( Bifidobacterium longum ) in one or more;

[0048] Described bacillus is: Bacillus licheniformis ( Bacillus licheniformis ), Bacill...

Embodiment 3

[0055] Embodiment 3: the preparation of Chinese medicine polysaccharide and Chinese medicine flavone

[0056] a. Codonopsis 15 parts, ginseng 18 parts, wolfberry 13 parts, Andrographis paniculata 20 parts, Achyranthes bidentata 15 parts, Poria cocos 20 parts, Ginkgo biloba 30 parts, licorice 20 parts, astragalus 20 parts, nettle 15 parts, quince 20 parts 15 parts of Salvia miltiorrhiza, weighed each raw material of Chinese medicine, chopped, washed, soaked in cold water overnight, then added purified water 15 times the weight of the raw material, water bathed to 90 ° C, and kept at 90 ° C, boiled for 2 hours, boiled Stir every 10 minutes during the process;

[0057] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0058] c. The supernatant in b is subjected to alcohol precipitation, the precipitation...

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Abstract

The invention discloses a preparation method of compound effervescent tablets for treating chicken infectious bursal disease, which belongs to the technical field of veterinary biological products. The preparation method comprises the following steps: carrying out passage and culture on a vaccine cell; reproducing a cell virus seed; reproducing a vaccine virus liquid; preparing a probiotics mixing bacteria liquid; freeze-drying a mixed preparation; and preparing the effervescent tablets. The preparation method has the beneficial effects that the requirement on transportation of the compound vaccine effervescent tablets for treating chicken infection bursal disease prepared by the invention is lowered and the convenience of users is improved; the compound vaccine effervescent tablets are capable of feeding microecologics while immunizing a chicken infectious bursal disease live vaccine, so that manpower and material resources are reduced and the stress of chicken flocks is reduced; the compound vaccine effervescent tablets are capable of improving the immunity of the chicken flocks, enhancing the overall immune effects of the chicken flocks and reducing the antibody dispersion of the chicken flocks, so that the antibody of the chicken flocks is kept on the same level and the infection risk of the chicken flocks is reduced.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a chicken infectious bursal disease compound effervescent tablet. Background technique [0002] With the rise of intensive poultry farming and the increase of international trade, poultry diseases are becoming more and more serious, resulting in high mortality of poultry, which seriously restricts the development of the poultry industry, causes serious losses to the world's poultry industry, and endangers economic development, food security and social stability. Chicken infectious bursal disease (Infection bursal disease, IBD) is a highly contagious disease of chickens and turkeys caused by infectious bursal virus (Infection bursal disease virus, IBDV). For young chickens at the age of one week, the virus mainly proliferates in the lymphocytes of the bursa of Fabricius, and causes varying degrees of damage to other immun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K9/46A61P31/14A61K31/715A61K35/744A61K35/742A61K35/745A61K35/747A61K36/815A61K36/537A61K36/484A61K36/481A61K36/344A61K36/258A61K36/21A61K36/19A61K36/185A61K36/16A61K36/11A61K36/076A61K31/593A61K31/525A61K31/51A61K31/375A61K31/355
Inventor 张秀文李阳闫艳丽刘雪王二先盛璐丝
Owner 浙江美保龙生物技术有限公司
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