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Method for producing microbial flocculant by using Paenibacillus mucilaginosus

A technology of microbial flocculant and Bacillus, applied in the field of preparation of microbial polysaccharides, achieves the effects of high purity and activity, shortened process time and high purity

Active Publication Date: 2014-03-19
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the post-treatment of microbial flocculants by macroporous adsorption resin

Method used

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  • Method for producing microbial flocculant by using Paenibacillus mucilaginosus
  • Method for producing microbial flocculant by using Paenibacillus mucilaginosus
  • Method for producing microbial flocculant by using Paenibacillus mucilaginosus

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Experimental program
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Effect test

Embodiment 1

[0043] The method for producing microbial flocculant by Paenibacillus colloidus comprises the following process steps:

[0044] A, adopt thermal shock method to carry out strain domestication to Paenibacillus mucilaginosus (Paenibacillus mucilaginosus) ACCC10013;

[0045] B. Paenibacillus colloidus ACCC10013 acclimated in Step A of aerated culture in a sterile fermentation medium containing carbon source, nitrogen source and growth factors to obtain a fermentation broth containing microbial flocculants;

[0046] C. Separating the fermented liquid containing the microbial flocculant prepared in step B by using a macroporous adsorption resin to prepare a microbial flocculant.

[0047] Described step A comprises following processing step:

[0048] A1, under aseptic conditions, add the bacterial species spores in the inclined plane of the test tube to sterile water, shake and wash the spores to make a spore suspension;

[0049]A2. Place the sterile container containing the spore...

Embodiment 2

[0078] The difference between this embodiment and embodiment 1 is:

[0079] The fermentation medium in the described step B consists of the following components in mass percentage:

[0080] Starch 20%, sucrose 10%, ammonium sulfate 2%, dipotassium hydrogen phosphate 2%, ferric chloride 0.5%, Tween-800.01%, yeast extract 3%, the balance is tap water, pH=7.5.

[0081] The culture condition in the described step B is:

[0082]

[0083] The amount of inoculum is the mass ratio of bacterial species: sterile fermentation medium = 4%-8%.

[0084] The preparation of the seed liquid is also included between the steps A and B, wherein the seed culture medium is composed of the following components in mass percentage:

[0085] Starch 5g, dipotassium hydrogen phosphate 5g, peptone 5g, yeast extract 5g, ammonium sulfate 0.5g, magnesium sulfate 0.5g, distilled water 1000ml, agar 18g.

[0086] The macroporous resin in the step C is selected from S-8 macroporous adsorption resin.

[00...

Embodiment 3

[0100] The difference between this embodiment and embodiment 1 is:

[0101] The fermentation medium in the described step B consists of the following components in mass percentage:

[0102] Starch 10%, sucrose 8%, ammonium sulfate 1%, dipotassium hydrogen phosphate 1.5%, ferric chloride 0.2%, Tween-800.005%, yeast extract 1%, the balance is tap water, pH=7.5.

[0103] The culture condition in the described step B is:

[0104]

[0105] The preparation of the seed liquid is also included between the steps A and B, wherein the seed culture medium is composed of the following components in mass percentage:

[0106] Starch 3g, dipotassium hydrogen phosphate 2g, peptone 3g, yeast extract 3g, ammonium sulfate 0.3g, magnesium sulfate 0.3g, distilled water 1000ml, agar 18g.

[0107] The macroporous resin in the step C is selected from S-8 macroporous adsorption resin.

[0108] Adopt macroporous adsorption resin to carry out post-treatment preferred technical scheme to the ferment...

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Abstract

The invention belongs to preparation of biological polysaccharide, and particularly refers to a method for producing microbial flocculant by using Paenibacillus mucilaginosus. The method comprises the following steps of: A, performing bacterial domestication on the Paenibacillus mucilaginosus ACCC10013 by means of thermal shock; B, aerobically culturing the Paenibacillus mucilaginosus ACCC10013 domesticated in the step A in a sterile fermental cultivation medium containing carbon source, nitrogen source and growth factors, thus obtaining a fermentation broth containing microbial flocculant; C, separating the fermentation broth containing microbial flocculant, prepared in the step B, through a macroporous adsorption resin to produce the microbial flocculant. The method provided by the invention solves the problems, such as high cost and low purity of flocculant, of the existing technology, and has the advantages of low technological cost of preparation process, high yield, high product purity, high activity, and the like.

Description

technical field [0001] The invention belongs to the preparation of microbial polysaccharides, in particular to a method for producing microbial flocculants by using Paenibacillus colloidus. Background technique [0002] Microbial flocculant is a non-toxic biopolymer compound prepared from microorganisms or their secretions with cohesive metabolites through the fermentation, extraction and purification of microorganisms and other processes by using modern biotechnology, mainly including glycoproteins, polysaccharides, Protein, cellulose and DNA, as well as bacteria with flocculation activity, etc. Microbial flocculants have the characteristics of wide flocculation range, high flocculation activity, safety, harmlessness, and pollution-free, and there are many types of flocculant-producing bacteria, which grow quickly and are easy to realize industrial production. At present, flocculants can be generally divided into inorganic flocculants, organic synthetic polymer flocculants...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C02F3/34C12R1/01
Inventor 章淑艳王云鹏赵从波秦艳梅李宾李军韩韬罗同阳郑翔刘丽娜
Owner 河北省微生物研究所有限公司
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