Method for extracting total flavones of hippophae rhamnoides from hippophae rhamnoides leaves

Abstract

The invention relates to a method for extracting total flavones of hippophae rhamnoides from hippophae rhamnoides leaves, and particularly relates to a method for extracting total flavones of hippophae rhamnoides by means of synergy of enzyme fermentation and continuous counterflow ultrasound. The method comprises the following steps: a, baking and grinding the hippophae rhamnoides leaves at 60 DEG C, and screening by a 40-mesh screen; b, fermenting by biological enzyme; c, performing continuous counterflow ultrasound assisted extraction; d, passing through a polyamide resin column, eluting by ethanol, collecting eluent, concentrating and drying to obtain the total flavones of hippophae rhamnoides. According to the method, the hippophae rhamnoides leaves are used as raw materials, and the overlaid effect of biological enzyme fermentation and ultrasound is sufficiently used, so that the solubility of total flavones of hippophae rhamnoides can be improved. The method has the advantages of simple process, short extracting time, mild reaction condition and the like, is environmentally friendly, is capable of protecting bioactivity of the total flavones of hippophae rhamnoides from damage, and is easy to realize industrial production.

Description

technical field

[0001] The invention relates to a method for extracting total flavonoids of seabuckthorn from seabuckthorn leaves, in particular to a method for extracting total flavonoids of seabuckthorn in cooperation with continuous countercurrent ultrasonic waves through enzyme fermentation. Background technique

[0002] Seabuckthorn is a wild berry plant belonging to the genus Hippophaeaceae, also known as vinegar willow, sandy date, and blackthorn. It is recognized as a precious edible and medicinal plant in the world. Studies have shown that the content of flavonoids in seabuckthorn in high altitude areas is relatively high, and the flavonoids in leaves mainly exist in the form of glycosides, mainly including four aglycones such as isorhamnetin, quercetin, kaempferol and myricetin. Its glycoside compounds have a wide range of pharmacological effects such as anti-myocardial ischemia and hypoxia, anti-arrhythmia, lowering serum cholesterol and enhancing immunity. [00...