Colloidal gold test strip for detecting sheep pox virus and preparation method thereof

A technology for colloidal gold test strips and sheep pox virus, which can be applied to measurement devices, instruments, scientific instruments, etc., and can solve the problems of complicated operation and time-consuming
CN104407130BInactive Publication Date: 2016-08-17ηŽ‹ζ΅·θ‰³
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
ηŽ‹ζ΅·θ‰³
Publication Date
2016-08-17
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a colloidal gold test strip for detecting goatpox virus and a preparation method thereof. The colloidal gold test strip for detecting goatpox virus comprises a sample pad, a colloidal-gold conjugate pad, a nitrocellulose membrane, an absorbent pad and a backing, wherein the sample pad, the colloidal-gold conjugate pad, the nitrocellulose membrane and the absorbent pad are successively adhered to a nonabsorbent support sheet; the colloidal-gold conjugate pad is a fiberglass membrane bonded to gold-labeled anti-goatpox virus P32 protein monoclonal antibody; and the nitrocellulose membrane has a detection line prepared by spraying of rabbit anti-P32 protein IgG and a quality control line prepared by spraying of goat anti-mouse polyclonal antibody. According to the invnetion, composition of a confining liquid used for preparation of the colloidal-gold conjugate pad and composition of a coating buffer solution used for preparation of the detection line and the quality control line are further optimized. It shows through specificity and sensitivity test results that the colloidal gold test strip for detecting goatpox virus has high specificity and high sensitivity.
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Description

technical field

[0001] The invention relates to a colloidal gold test strip, in particular to a colloidal gold test strip for detecting sheep pox virus and a preparation method thereof, belonging to the detection field of sheep pox virus. Background technique

[0002] Goat pox is an acute, febrile, contagious disease of sheep or goats caused by sheep pox virus or goat pox virus (GTPV) of the genus Capipoxvirus. The main manifestations are fever, papules and herpes on the skin or mucous membranes of hairless or less hairy parts. Goat pox is the most serious of all animal pox diseases, with a high fatality rate and large economic losses, seriously affecting the development of international trade and sheep farming. In addition, this disease is also of great significance in public health. China, Mongolia, Sweden, and India all have reports of human infection with sheeppox virus, which has been highly valued by countries all over the world. Import and export of susceptible anim...

Claims

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