Pullorum agar gel precipitating antigen as well as preparation method and application thereof

Abstract

The invention discloses a pullorum agar gel precipitating antigen as well as a preparation method and an application thereof, and belongs to the technical field of veterinarian diagnosis. Salmonella pullorum SP8441 and salmonella pullorum SP9905 are subjected to recovery and passage respectively to form a seed bacterium liquid, then the seed bacterium liquid is inoculated with a solid culture medium for proliferation, after a culture solution is removed centrifugally, bacterial protein is extracted with a lysis buffer, and the pullorum agar gel precipitating antigen is prepared. The technological method is simple, reasonable, scientific, stable in production and low in cost, the prepared pullorum agar gel precipitating antigen product has the advantages of high sensitivity and high specificity, the pullorum purification process can be simplified, the detection application range can be enlarged, and the agar gel precipitating antigen is relatively ideal in pullorum detection.

Description

technical field [0001] The invention belongs to the technical field of veterinary diagnosis, and in particular relates to a chicken pullorum agar expansion antigen and a preparation method and application thereof. Background technique [0002] Pullorum is the most serious bacterial infectious disease in poultry farming. Its pathogen is Salmonella pullorum. The disease can be transmitted vertically through eggs, and can also be transmitted horizontally through respiratory tract, digestive tract, conjunctiva and damaged skin wounds. After Salmonella pullorum infection, it can survive in the host body for a long time until it colonizes the ovary, fallopian tube, testis and other sexual organs after sexual maturity, and produces a high level of agglutination antibody continuously. Whole blood (serum) and elimination of positive chickens can cut off the vertical transmission of pathogens. However, in actual production, there are still defects in the use of pullorum agglutinated ...

AI Technical Summary

Problems solved by technology

However, in actual production, there are still defects in the use of pullorum agglutinated antigen for quarantine and purification. For example, individuals who are weakly positive in the agglutinated antigen test result still need to be quarantined again after an interval of 2-3 weeks, which not only increases the cost of feeding, but also prolongs the time. The purification time is shortened, and the risk of horizontal transmission of the disease is also increased; since Salmonella pullorum infection of young chickens cannot reproduce in large quantities in the body, it usually does not produce high levels of agglutinated antibodies, resulting in the detection of agglutinated antigens on young chickens. It is not conducive to the early diagnosis of diseases; when using agglutinated antigens to detect yolk, there are disadvantages such as low sensitivity, slow diagnosis speed, and unclear agglutination images, which limit the application of agglutinated antigens in fields such as quarantine of imported chicken breeding eggs

It can be seen that the existence of the above problems has seriously affected the effect of pullorum quarantine and purification, resulting in the inability of the breeder flocks after quarantine to achieve the goal of thorough purification, which has caused serious economic losses to the breeders.

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