Preparation method of fibroin and graphene oxide composite bracket material

A graphene composite and scaffold material technology, applied in the field of biomedical materials, can solve problems such as lack of mechanical properties, and achieve the effect of good effect, good air permeability, and huge specific surface area

Inactive Publication Date: 2015-08-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these related studies, the prepared composite materials are all non-porous or less porous membrane materials, or porous scaffold materials with poor mechanical properties.
There has never been a report of blending silk fibroin aqueous solution with graphene oxide, and then preparing a bioscaffold material with excellent mechanical properties and high porosity, which is used for the repair of human wounded skin, defective bone and other human tissues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Dissolving the fibrous silk fibroin formed by degumming silkworm cocoons in 9.3 mol / L LiBr solution, filtering, dialysis, centrifuging, and then concentrating to 10% silk fibroin aqueous solution by mass percentage.

[0032] (2) 0.1mg / mL graphene oxide aqueous solution is ultrasonically dispersed, and the graphene oxide aqueous solution is mixed with the silk fibroin solution (the mass ratio of graphene oxide to silk fibroin after mixing is 1:1000), so that the graphene oxide is dispersed In the silk fibroin solution, stir evenly, and vibrate for 5 minutes under ultrasonic waves, so that graphene oxide and silk fibroin are cross-linked with each other to obtain a mixed solution.

[0033] (3) Freeze the solution obtained in (2) at -20° C. and then dry it with a freeze dryer to obtain a spongy composite scaffold material of graphene oxide and silk fibroin.

[0034] (4) The dissolution experiments showed that compared with the silk sponge prepared at the same concentra...

Embodiment 2

[0036] (1) The fibrous silk fibroin formed after silkworm cocoon degumming is filtered, dialyzed, centrifuged in 50% (w / w) CaCl2 solution, and then concentrated to a 10% silk fibroin aqueous solution by mass percentage.

[0037] (2) Ultrasonically disperse 1 mg / mL graphene oxide aqueous solution, and vacuum filter; mix the silk fibroin solution in (1) with it (the mass ratio of graphene oxide to silk fibroin after mixing is 1:50), so that The silk fibroin solution was dispersed in the graphene oxide solution, stirred evenly for a long time, and oscillated under ultrasonic waves for 10 minutes, so that the graphene oxide and silk fibroin were cross-linked with each other, and then centrifuged at a low speed for 20 minutes to obtain a mixed solution.

[0038] (3) The mixed solution obtained in (2) was prepared by a freeze-drying method to obtain a porous silk fibroin / graphene oxide film-like material.

[0039] (4) The mechanical test results show that compared with the silk fibr...

Embodiment 3

[0041] (1) dissolving the fibrous silk fibroin formed after silkworm cocoon degumming, filtering, dialysis, centrifuging, and then concentrating to a silk fibroin aqueous solution with a mass percentage of 5%;

[0042] (2) Add 2 mg / mL graphene oxide aqueous solution to SDS solution with a mass fraction of 1%, then ultrasonically disperse for 15 minutes, and vacuum filter;

[0043] (3) Mix the silk fibroin solution in (1) with the graphene oxide solution in (2) (the mass ratio of graphene oxide and silk fibroin after mixing is 1:50), so that the silk fibroin solution is evenly dispersed in the oxidized In the graphene solution, stir evenly for a long time, vibrate for 20 minutes under ultrasonic waves, so that the graphene oxide and silk fibroin are cross-linked, and then centrifuge at a low speed for 5 minutes to obtain a mixed solution.

[0044] (4) Pour the mixed solution obtained in (3) into a cylindrical mold, and prepare a porous silk fibroin / graphene oxide three-dimensio...

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Abstract

The invention discloses a preparation method of a fibroin and graphene oxide composite bracket material. The preparation method comprises the steps of: performing dissolution, filtration, dialysis and centrifugation on degumming fibrous fibroin in sequence, and then condensing to obtain a silk fibroin aqueous solution; dispersing graphene oxide aqueous solution ultrasonically; filtering in a vacuum manner to remove impurities; mixing the graphene oxide aqueous solution with the silk fibroin solution; mixing uniformly; oscillating at the presence of ultrasonic waves for mutual crosslinking; then centrifuging to obtain a mixed solution; then preparing by adopting a freeze-drying method so as to obtain the porous fibroin and graphene oxide composite bracket material. The high-concentration fibroin and the graphene oxide blended aqueous solution has good mixing property, the obtained composite bracket material is excellent in air permeability and drug-carrying ability, is good in hygroscopic property and waste liquid removal effect, and has wide application foreground.

Description

technical field [0001] The invention relates to a preparation method of a composite material, in particular to a preparation method of a silk fibroin and graphene oxide composite scaffold material, and belongs to the technical field of biomedical materials. Background technique [0002] As a natural fibrous protein, silk fibroin contains 18 kinds of amino acids that are of great nutritional value to the human body. Because of its unique mechanical properties, good biocompatibility, degradation controllability, antibacterial properties and stability, silk fibroin And low immunogenicity, it has a wide range of applications in the field of biomaterials, such as can be prepared into film, porous scaffold, gel, powder, fiber and other forms, used in immobilized enzymes, antigen antibody carriers, drugs Research on sustained-release carriers and anticoagulant materials. The regenerated silk film has strong adhesion to cells, and epidermal cells, fibroblasts, mesenchymal stem cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/40A61L27/22A61L27/54A61L15/32A61L15/44
Inventor 杨明英帅亚俊毛传斌
Owner ZHEJIANG UNIV
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