Serum-free culture medium used for cultivating human mesenchymal stem cells

Abstract

The invention discloses a serum-free culture medium used for cultivating human mesenchymal stem cells. DMEM culture solution is taken as a basic culture medium, full nutritional components, namely amino acid, vitamin, protein nutrients and inorganic salt, and regulatory components, namely a glycoregulatory hormone, a growth factor and an antioxidant, are added into the DMEM culture solution. The serum-free culture medium used for cultivating the human mesenchymal stem cells can be used for cultivating the human mesenchymal stem cells with low cost, comprehensive nutrition is provided for the human mesenchymal stem cells, and growth of the human mesenchymal stem cells is effectively regulated and controlled, so that the human mesenchymal stem cells have good activity in a cultivation process, a mescenchymal stem cell cultivation process is improved, requirement on animal serum in the mesenchymal stem cell cultivation process is met, and animal protein residue and animal-sourced virus infection probability are reduced.

Description

technical field [0001] The invention relates to a serum-free culture medium, which belongs to the field of biochemistry. Background technique [0002] Due to their good self-proliferation ability and multi-directional differentiation potential, mesenchymal stem cells have the ability to differentiate into osteoblasts, chondrocytes, adipocytes, endothelial cells, nerve cells, muscle cells, liver cells, etc. under specific conditions in vivo and in vitro. The ability of adult cells to differentiate. Existing studies have shown that mesenchymal stem cells have immune regulation and hematopoietic support functions, and are easy to introduce and express foreign genes. Therefore, they can be used as seed cells in the construction of tissue engineered bone, cartilage and cardiac muscle, and important carrier cells in gene therapy. It also has broad application prospects in hematopoietic stem cell transplantation and organ transplantation. [0003] Due to the above-mentioned needs...

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Problems solved by technology

This culture method will affect cell proliferation efficiency and biological performance, and there is a risk of virus contamination caused by poor animal serum quality, resulting in foreign protein contamination of cell products

[0004] In view of the above problems, a solution proposed in the field is to replace the medium containing fetal bovine serum with serum-free medium when human mesenchymal stem cells are cultured to the last 1-2 passages, but residual foreign protein contamination always exists , cannot be completely removed; another solution is to use serum-free medium for the whole culture, which requires sufficient nutrients in the serum-free medium to maintain the good growth of cells, resulting in high-quality serum-free culture of human mesenchymal stem cells The cost of the base is extremely high, and at the same time, if the nutrient composition ratio design is unreasonable, the stem cell culture will be out of control, making it difficult for large-scale industrial application, and it is also difficult to control the quality of human mesenchymal stem cell products

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