Lycium ruthenicum Murr tea drink and preparation method thereof
A technology of black fruit wolfberry and tea drinks, which is applied in the directions of tea substitutes, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of poor flavor and efficacy of black fruit wolfberry extract, and achieve unique flavor and enhance immunity. Power and drinking convenience
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Embodiment 1
[0054] (1) Soak black wolfberry in water, crush and beat, add culture medium after sterilizing the slurry to obtain fermentation substrate, the composition of culture medium is 3% glucose, 1% peptone, 1% soybean powder, 3% agar, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.3%, the balance is water;
[0055] (2) After the rice wine yeast is expanded and cultivated, it is inoculated into the fermentation substrate for fermentation; the fermentation temperature is 27° C., and the fermentation time is 20 days;
[0056] (3) extracting the fermented liquid, freeze-drying, pulverizing, and performing supercritical carbon dioxide extraction to obtain the black fruit Lycium barbarum extract. The pressure of supercritical carbon dioxide extraction is 18MPa, the temperature is 40°C, the extraction time is 3h, the flow rate of carbon dioxide is 10L / h, and the entrainer is ethanol.
Embodiment 2
[0058] (1) Soak black wolfberry in water, crush and beat, sterilize the slurry, add culture medium to obtain fermentation substrate, the composition of the culture medium is 2% sucrose, 3% peptone, 2% soybean powder, 1% bran, sulfuric acid Magnesium 0.1%, potassium dihydrogen phosphate 0.3%, dipotassium hydrogen phosphate 0.1%, the balance is water;
[0059] (2) After expanding the culture of Lactobacillus delbrueckii, it is inoculated into the fermentation substrate for fermentation; the fermentation temperature is 23° C., and the fermentation time is 24 days;
[0060] (3) extracting the fermented liquid, drying it with microwaves, pulverizing it, and performing supercritical carbon dioxide extraction to obtain the black fruit Lycium barbarum extract. The pressure of supercritical carbon dioxide extraction is 33MPa, the temperature is 28°C, the extraction time is 2h, the flow rate of carbon dioxide is 25L / h, and the entrainer is ethanol.
Embodiment 3
[0062] (1) Soak black wolfberry in water, crush and beat, add culture medium after the slurry is sterilized, and obtain fermentation substrate, the composition of culture medium is fructose 4%, beef extract 2%, potassium dihydrogen phosphate 0.06%, zinc sulfate 0.04 %, magnesium chloride 0.05%, and the balance is water;
[0063] (2) After the Lactobacillus acidophilus is expanded and cultivated, it is inoculated into the fermentation substrate for fermentation; the fermentation temperature is 32° C., and the fermentation time is 15 days;
[0064] (3) Extract the fermented liquid, dry it at 50° C., crush it, and perform supercritical carbon dioxide extraction to obtain the black fruit Lycium barbarum extract. The pressure of supercritical carbon dioxide extraction is 20MPa, the temperature is 35°C, the extraction time is 5h, the flow rate of carbon dioxide is 8L / h, and the entrainer is ethanol.
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