Lactobacillus plantarum and application thereof
A technology of Lactobacillus plantarum and strains, applied in the field of microorganisms, can solve problems affecting blood supply and blood supply of tumor cells, and achieve high safety and health care effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0015] Embodiment 1, collection and separation of plantarum lactobacillus ZWQ-9
[0016] The sample is yogurt, a national characteristic dairy product collected from Aheqi County, Kizilsu Kirgiz Autonomous Prefecture, Xinjiang. Store the collected samples in a low-temperature environment at 4°C; carry out the separation operation of lactic acid bacteria in the laboratory: take 10mL of yoghurt sub-sample, add 90mL of sterile normal saline to dilute it in a conical flask, stir well, fully dissolve and place in a shaker Shake it up for 20 minutes to fully mix the sample with water and disperse the bacteria, which is 10 -1 Sample diluent; draw 1mL10 -1 Inject the sample diluent into a test tube filled with 9mL sterile saline, mix well, and dilute to 10 -4 , select 10 -2 、10 -3 、10 -4 Three dilution gradients were streaked and inoculated on the MRS-calcium carbonate agar medium plate, and cultured at 37°C for 48 hours; observe the shape of the colonies, such as round slightly ...
Embodiment 2
[0017] Embodiment 2, identification of plantarum lactobacillus ZWQ-9
[0018] Refer to the "Common Bacteria System Identification Handbook" edited by Dong Xiuzhu, Cai Miaoying, etc., "Lactic Acid Bacteria Classification and Identification and Experimental Methods" edited by Ling Daiwen, etc., and "Lactic Acid Bacteria Modern Research Experimental Technology" edited by Guo Xinghua, etc. to conduct physiological and biochemical tests on the isolated lactic acid bacteria. Index detection, including gelatin liquefaction test, nitrate reduction test, hydrogen sulfide production test, indole test, motility test, starch hydrolysis test, lactic acid optical rotation test, glucose gas production test, casein hydrolysis test, V-P test and sugar fermentation test etc.
[0019] At the same time, the 16SrRNA gene sequence analysis was carried out on the isolated lactic acid bacteria. The specific operation is:
[0020] SDS method to extract strain genomic DNA:
[0021] (1) Take 1 mL of ...
Embodiment 3
[0036] Embodiment 3, the determination of exopolysaccharide of Lactobacillus plantarum ZWQ-9
[0037] Extraction of extracellular polysaccharides from Lactobacillus plantarum ZWQ-9: use MRS agar medium to activate the isolated Lactobacillus, and culture at 37°C for 24 hours; the activated strain is then fermented with MRS broth, and the fermentation condition is 37°C for more than 48 hours . Centrifuge the fermentation broth (15min, 12000×g, 4°C) to remove bacteria and impurities; add trichloroacetic acid with a mass concentration of 80g / 100mL to the supernatant to a final mass concentration of 4g / 100mL, let it stand for 4-8h, and centrifuge (15min, 12000r / min, 4°C) to remove the precipitated protein; add absolute ethanol to the supernatant to a final volume fraction of 75%, let stand at 4°C for 12h, centrifuge to remove the precipitate, dissolve in deionized water, and centrifuge to remove the precipitate; the supernatant Liquid deionized water was dialyzed for 3 days, and t...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More