Method for extracting microalgae lipid through biological enzyme catalysis wall breaking

A technology of microalgal oil and biological enzymes, which is applied in the production of fat, fat oil/fat refining, fat oil/fat production, etc., can solve the problems of large energy input, high requirements for oil extraction equipment, and insurmountable problems, so as to reduce energy input. Effect

Inactive Publication Date: 2016-02-03
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned invention still cannot overcome the technical bottlenecks of the current heterotrophic oleaginous microalgae in terms of wall break

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] A new type of biological enzyme catalyzed breaking the wall to extract microalgae oil, the steps are:

[0025] A. Weigh out 10g of freeze-dried algae powder obtained by heterotrophic fermentation and culture, place 10g of Chlorella freeze-dried algae powder into a stoppered Erlenmeyer flask, add 50ml of 0.05M phosphate buffer solution (PH7.2) and mix well with the algae, then add 150mg of chitin The composite enzyme (1:1:1, m / m / m) composed of enzyme, cellulase and lipase is shaken and placed in a water bath at 50°C for 4 hours.

[0026] B. Further, adjust the pH value of the system to 7.5 or 8.5 or 9 with 0.1M sodium hydroxide solution and increase the temperature of the water bath to 70 or 75 or 80°C and maintain it for 2 hours. After the reaction is completed, cool the flask to below 40°C, then add 10ml of a mixed solvent of n-hexane and ether (1:1, v / v) and transfer to the centrifuge tube to shake vigorously for extraction, then add 20ml of methanol an...

Example Embodiment

[0028] Example 2:

[0029] A new type of biological enzyme catalyzed breaking the wall to extract microalgae oil, the steps are:

[0030] A. Take 500g of photosynthetic autotrophic chlorella fresh algae mud, place the algae mud with a solid content of 21.0% in a three-necked bottle, add 400ml of 0.05M phosphate buffer solution (PH7.2) and stir well with the algae mud, then add 2.0g composite enzyme (1:1:1, m / m / m) composed of chitinase, pectinase and alkaline protease, stir it evenly, and place it in a water bath at 50°C for 4 hours.

[0031] B. Further, adjust the pH value of the system to 7.8 or 8.3 or 9 with 0.1M sodium hydroxide solution and increase the temperature of the water bath to 70 or 73 or 76 or 80°C and maintain it for 2 hours. After the reaction is completed, cool the Erlenmeyer flask to below 40°C, then add 150ml of a mixed solvent of n-hexane and ether (1:1, v / v) to the three-neck flask for extraction with vigorous shaking, then add 200ml of methanol and continue sh...

Example Embodiment

[0034] Example 3:

[0035] A new type of bio-enzyme catalyzed wall-breaking method for extracting microalgae oil, the steps are:

[0036] A. Take 20L of heterotrophic inferior chytrid algae fermentation broth and place it in a glass extraction kettle. The solid content of the fermentation broth is 108g / L. Add 4.8g of potassium dihydrogen phosphate and 36g of dipotassium hydrogen phosphate in turn and stir to dissolve evenly. Add 32g of a complex enzyme (1:1, m / m) composed of chitinase and alkaline protease, stir well, and then heat to 45°C for 3 hours.

[0037] B. Further, adjust the pH of the system to 7.5 or 7.8 or 8 with 0.1M sodium hydroxide solution, and increase the temperature of the water bath to 70 or 74 or 78 or 80°C and continue to maintain it for 1 hour. After the reaction was completed, 5L ethanol and 3L n-hexane were added to the above system and stirred and extracted for 30 min.

[0038] C. A three-phase disc separator is further used to separate the oil phase, and th...

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Abstract

The invention discloses a method for extracting microalgae lipid through biological enzyme catalysis wall breaking. The method includes the steps that A, a phosphate buffer solution and a biological enzyme catalyst are sequentially added into wet algae mud, or algae slurry, or dry powder or fermentation liquor for culturing harvested lipid-generating microalgae, and the mixture is stirred and heated; B, after the enzyme catalysis degradation reaction is finished, the pH value of a reaction system is adjusted, and the temperature of enzyme catalysis degradation liquid is raised and kept; C, after the reaction is finished, the temperature of the enzyme catalysis degradation system is lowered, an extraction agent is added into the reaction system for lipid extraction, and then demulsifier is added to carry out layering through centrifugation; D, reduced pressure distillation or thin film evaporation concentration is carried out on the oil phase obtained through separation to obtain the microalgae lipid. The method is easy to implement and convenient to operate; due to efficient wall-breaking lipid extraction for the lipid-generating microalgae particularly photoautotroph lipid-generating microalgae, energy input in the microalgae lipid extraction process is reduced, the energy efficiency ratio of microalgae energy is increased, and then comprehensive production cost of microalgae biomass energy is greatly reduced.

Description

technical field [0001] The invention relates to the fields of microalgae biochemical industry, microalgae biomass energy, microalgae high value-added natural product chemistry and the like, in particular to a cell wall breaking and oil extraction method of oil-producing microalgae. Background technique [0002] With the rising price of fossil fuels and the recognition of the non-renewability of fossil fuels, people's demand for alternative new energy sources is also expanding. Microalgal photosynthetic autotrophy can reduce CO under normal temperature and pressure 2 Perform efficient absorption, convert light energy into fat or starch, store it in cells, and release oxygen. Microalgae have extremely high photosynthetic efficiency, efficient concentration and fixation of CO 2 The ability and the ability to use non-food crop land such as oceans, tidal flats, and deserts as breeding sites are considered to be a very promising bulk raw material for biodiesel production in the ...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/10C11B3/12
Inventor 陈伟宋立荣吴莎
Owner INST OF AQUATIC LIFE ACAD SINICA
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