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Rhizobium radiobacter for preventing plant root cancer and application thereof

A radiorhizobium, plant root cancer technology, applied in forest and horticultural crop protection, agricultural field

Active Publication Date: 2016-02-17
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the widespread occurrence of root cancer caused by A. tumefaciens on fruit trees and other plants, there is currently no registered biocontrol agent in my country

Method used

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  • Rhizobium radiobacter for preventing plant root cancer and application thereof
  • Rhizobium radiobacter for preventing plant root cancer and application thereof
  • Rhizobium radiobacter for preventing plant root cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1, Isolation and Identification of Radial Rhizobium (Rhizobiumradiobacter) PAR

[0065] 1. Screening of R.radiobacter strain PAR

[0066] Radial rhizobia (R.radiobacter) PAR of the present invention adopts dilution plate method and plate streaking method to separate and obtain from peach tree rhizosphere soil, and separation method is:

[0067] (1) Isolation of radial rhizobia: the collection of soil samples, in the Xiyuan gardening field of Tai'an City, Shandong Province, collected fibrous roots and sticky rhizosphere soil of different varieties of healthy peach trees in peach orchards, indicating the collection location, time and collection people. Weigh 1g of peach tree fibrous root, put it into 99ml of sterile water, shake it well for 30min. Then use a pipette gun to draw 1ml from the triangular flask, add it to another test tube filled with 9ml sterile water, mix well, and so on to make 10ml -3 、10 -4 、10 -5 Soil solutions of different dilutions. Take...

Embodiment 2

[0090] Embodiment 2, the antibacterial activity assay of Radial Rhizobium (Rhizobiumradiobacter) PAR and the preparation of bacterial agent

[0091] 1. Determination of antibacterial activity of Rhizobium radiobacter PAR

[0092] Agrobacterium tumefaciens (A. tumefaciens) ATCC23308 was inoculated on the NA (see above for the recipe) slant, and cultured at 28°C for 48h. Then add an appropriate amount of sterile water to the slope to prepare a suspension of pathogenic bacteria. Take 1ml bacterial suspension (10 8 cfu / ml) placed in a sterilized petri dish, poured into YEB culture medium melted at 40-50°C, and shaken evenly to make an antagonist assay plate with bacteria. The rhizobium radiobacter (Rhizobium radiobacter) PARCGMCC No. 11639 obtained in Example 1 and cultured for 24 hours was picked and spotted on the antagonism assay plate. After culturing at 28°C for 1-2 days, observe the colony morphology and the formation of the inhibition zone, and measure the diameter (mm) ...

Embodiment 3

[0100] Embodiment 3, the inhibitory action of Radial Rhizobium PAR bacterial strain to Agrobacterium tumefaciens (A. tumefaciens) infecting sunflower to form root tumor nodules

[0101] Mix the Rhizobium radiobacter PARCGMCC No.11639 and A. tumefaciens ATCC23308 cultivated to the logarithmic phase in the nutrient broth (recipe above) at a volume ratio of 10:1 (V / V) , in the mixed solution, the number of live bacteria of Rhizobium radiobacter (Rhizobiumradiobacter) PARCGMCCNo.11639 is 10 6 Individual / ml, the number of live bacteria of Agrobacterium tumefaciens (A. tumefaciens) ATCC23308 is 10 5 pieces / ml. The mixture was inoculated to the cotyledon of sunflower about 10 days after emergence, and the inoculum volume was 20 μL. For the specific method, refer to Wang Hongyan (reference: Wang Hongyan. Cherry and Cherry Blossom Root Cancer Pathogen and Biological Control Research: [Master's Thesis] China Agricultural University, 1998, p7.) method.

[0102] The bacterial suspensio...

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Abstract

The invention discloses rhizobium radiobacter for preventing plant root caner and an application thereof. The rhizobium radiobacter is specifically rhizobium radiobacter PAR, and a preservation number of the rhizobium radiobacter PAR in China General Microbiological Culture Collection Center is CGMCC No. 11639. The strain is similar to the nutritional need of the root cancer A tumefaciens, is rapid to grow, can be rapidly mass fixed at roots of a plant and has a niche advantage; the strain PAR does not generate antibiotics, so that the drug fastness of the root cancer A tumefaciens can be avoided, and the strain PAR is more advantageous than the rhizobium radiobacter generating the antibiotics; in addition, the strain can greatly prevent the A tumefaciens from affecting sunflowers, tomatoes and peach tree not to form root tumors, and the application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of agriculture, forestry and horticultural crop protection, and relates to a radial rhizobia strain for preventing and treating plant root cancer and its application. Background technique [0002] Root cancer is a common bacterial disease caused by Agrobacterium tumefaciens. Agrobacterium tumefaciens has a wide host range and can infect 643 species of plants belonging to 93 families and 331 genera. It occurs commonly in some countries such as Europe, North America, South Africa and Asia. It occurs to varying degrees in peach cultivation areas in my country, with an incidence rate of 20% to 90% (Ma Deqin, Wang Huimin. Fruit tree root cancer and its biological control [J]. Chinese Fruit Tree, 1995, Issue 02). More serious root cancer occurred in Hebei, Shandong, Liaoning, Henan, Hubei, Jiangsu and other provinces. Peach tree seedlings suffer premature aging, plant dwarfing, and even death; mature trees infe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01C1/06A01G13/00C12R1/01
Inventor 李世访郭荣君李茜高之蕾李世东
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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