An electrospray ionization device and its application

A technology of electrospray ionization and conductive clips, applied in the direction of measuring devices, ion sources/guns, circuits, etc., can solve problems such as opacity, limited applications, poor biocompatibility, etc., achieve high biocompatibility, improve The effect of precision and repeatability

Inactive Publication Date: 2018-05-18
CHINA UNIV OF GEOSCIENCES (BEIJING)
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the poor biocompatibility of these paper genes makes it difficult for cells to attach to them, and their opacit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An electrospray ionization device and its application
  • An electrospray ionization device and its application
  • An electrospray ionization device and its application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1 Manufacturing method and structure of electrospray ionization device

[0049] (Production of glass substrate)

[0050] Cut a cover glass with a thickness of 20μm with a glass knife into an isosceles triangle with a waist length of 0.8cm and an apex angle of 45°. After washing and drying, spin a layer on the surface with a homogenizer at 1000rpm The SU-82015 negative photoresist with a thickness of 30μm is baked at 65°C for 20min, and a mask with a microchannel pattern (the width of the microchannel is 2mm, the number is 3) is covered on the glass slide, After ultraviolet exposure, development, and nitrogen drying, a glass substrate with 3 microchannels with a width of 2 mm and a depth of 30 μm is obtained.

[0051] (Production of PDMS chamber)

[0052] The PDMS prepolymer and the initiator are mixed at a mass ratio of 10:1 and then poured on the silicon wafer. The silicon wafer is sealed in advance with a wide tape to prevent the PDMS prepolymer from leaking. After ...

Example Embodiment

[0054] Example 2 Co-cultivation of cells in microchannels

[0055] 1) Digest the human liver tumor cells HepG2, human cloned colon adenocarcinoma cell Caco-2 and human breast cancer cell MCF-7 cells that are overgrown in a 60mm petri dish, and dilute them with culture medium to 10 6 mL -1 Cell suspension. Take 10 μL of the cell suspension of the three kinds of cells and add them to the three microchannels on the glass substrate in the PDMS chamber (e.g. figure 2 (Shown), after the cells adhere to the wall, add some new medium, put the glass substrate placed in the PDMS chamber into the cell incubator, and culture overnight.

[0056] 2) Take out the glass substrate from the incubator, stain HepG2, Caco-2 and MCF-7 cells with three different fluorescent dyes, Cell TrackerTM Green CMFDA, CellTrackerTM Deep Red and Cell TrackerTM Violet, and place them in a confocal laser Observe under the microscope, the results are as image 3 Shown. It can be seen that due to the surface tension o...

Example Embodiment

[0058] Example 3 Qualitative mass spectrometry detection of drug absorption in cells

[0059] Co-culture HepG2, Caco-2 and MCF-7 cells in the same manner as step 1) of Example 2. After culturing overnight, remove the glass substrate placed in the PDMS chamber from the incubator, and add cyclophosphamide dropwise The solution (hereinafter referred to as CPA, the concentration is 10 mmol / L, that is, 10 mM, the dosage is 10 μL), and then put it back into the incubator for 24 hours.

[0060] A glass substrate with MCF-7 cells (ie, monocultured MCF-7 cells) cultured in all three microchannels was used as a control sample, and the culture mode and drug action mode were the same as the co-cultured MCF-7 cells.

[0061] Remove the glass substrate placed in the PDMS chamber from the cell incubator, clamp the glass substrate, and rinse with distilled water three times to remove the complex matrix remaining on the surface of the glass slide. After the glass substrate is naturally dried, place ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Depthaaaaaaaaaa
Side lengthaaaaaaaaaa
Login to view more

Abstract

The invention relates to an electrospray ionization device, comprising: a glass substrate having at least one tip, on which a plurality of microchannels are arranged; a conductive clip for applying a high voltage and clamping the glass substrate; and for placing the glass-based chamber. The invention also relates to the application of the electrospray ionization device in cell-based drug analysis. The glass substrate provides high biocompatibility, which can be used for qualitative and quantitative analysis of drugs in cells, and can also be used for biological analysis of cells after drug action.

Description

technical field [0001] The invention relates to the field of cell-based drug analysis, in particular to an electrospray ionization device and its application in cell-based drug analysis. Background technique [0002] Cell-based drug analysis has always played an important role in the development of biomedicine and pharmacy. Traditional cell-based drug analysis methods require the cultivation of a large number of cells and complex operating procedures to obtain sufficient data. These traditional analytical methods often have the disadvantages of high reagent consumption, time-consuming and low throughput. [0003] Mass spectrometry is a relatively fast analytical method that can provide chemical information with high sensitivity, high throughput and molecular level. As a multifunctional analytical technique, mass spectrometry can be used in conjunction with electrophoresis, chromatography, etc. to separate and extract relevant pharmaceutical components, thereby improving th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): H01J49/16G01N27/62
CPCH01J49/165G01N27/62
Inventor 吴静
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap