High-copper feed for establishing HLD (hepatolenticular degeneration) animal model and preparation method of high-copper feed

Abstract

The invention discloses high-copper feed for establishing an HLD (hepatolenticular degeneration) animal model and a preparation method of the high-copper feed and relates to the technical field of establishing of an animal model for a medicine experiment. According to the preparation method, soybean oil and cocoa butter are weighed in proportion and heated to be melted to a semi-flow fat material; raw materials are weighed and comprise casein, corn starch, maltodextrin, cane sugar, cellulose, L-cystine, composite mineral substances, anhydrous calcium hydrogen phosphate, calcium carbonate, citric acid monohydrate, multivitamins and choline chloride; the weighed raw materials are mixed and made into feed granules with the diameter of 10 mm and the length of 10-20 mm by a hoop standard granulator; a dehumidifier is used for dehumidification and drying for 24-48 h, and finally, the feed granules are obtained after drying; a product is packaged and subjected to irradiation sterilization with cobalt 60, and the finished product is obtained. The product contains stable nutrient ingredients and meets demands for hardness and taste according to the feeding habit of rodents; the model success rate is high, the process is simple and the modeling effect is good.

Description

technical field [0001] The invention relates to the technical field of building animal models for medical experiments, and relates to a high-copper feed for building animal models of hepatolenticular degeneration and a preparation method thereof. Background technique [0002] Hepatolenticular degeneration (HLD), also known as Wilson disease, is an autosomal recessive genetic disease and a congenital disorder of copper metabolism. Clinically, liver damage, kidney damage, extrapyramidal symptoms and corneal pigment rings are the main manifestations. [0003] Copper is an essential trace element for the human body and participates in the synthesis of many important biological enzymes as a prosthetic group. A normal adult takes 2-5 mg of copper daily from the diet, about 30% is absorbed into the blood in the stomach, duodenum and upper jejunum, and most of it is loosely combined with albumin and enters the liver cells, where copper is firmly bound to α2 globulin Combined into ...

AI Technical Summary

Problems solved by technology

[0006] At present, high-dose copper sulfate solution is commonly used in China to gavage rodents to make animal models of hepatolenticular degeneration; The process will cause certain irritation to the animal's body, especially the digestive tract is easily injured and the drug is easily mistaken into the trachea, resulting in abnormal death of the animal due to respiratory suffocation, and the use of gavage method is not suitable for rodents The eating habits of animals free feeding; 2. The staff not only have a large workload during the operation, but also increase the workload of the staff. The long-term operation is prone to errors, which leads to increased animal mortality and increased experimental results. 3, gavage method easily causes experimental animals to produce stress response, and also easily causes unstable phenotype, experimental data has deviation and molding rate is low; The present invention feeds For rodents, nutrient-stable feed composed of single and stable nutrients is used in the preparation process. Such feed pellets have the characteristics of good molding effect, strong hardness, and not easy to break, which not only ensures the food intake of animals, but also better guarantees The grinding process of rodents themselves; the hardness and mouthfeel conform to the eating habits of rodents, and the animal model of hepatolenticular degeneration induced by the present invention has a high success rate

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