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A thermotolerant Bacillus amyloliquefaciens ytk1 and its application in tobacco leaf curing

A starch spore-decomposing and high-temperature-resistant technology, applied in the field of microorganisms, can solve the problems of insufficient starch degradation of tobacco leaves, changes in the way of water loss of tobacco leaves, and incomplete sugar conversion, etc., so as to improve the quality of tobacco, increase the brightness and luster, and reduce the burnt smell. Effect

Active Publication Date: 2019-05-07
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dense barns are ventilated by mechanical equipment. Due to the high wind speed between leaves and forced dehumidification, the distribution of temperature, humidity and wind speed in the smoke layer will be affected, resulting in changes in the way the tobacco leaves lose water. , the reason is that the starch degradation of tobacco leaves is not sufficient, and the sugar conversion is not complete.

Method used

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  • A thermotolerant Bacillus amyloliquefaciens ytk1 and its application in tobacco leaf curing
  • A thermotolerant Bacillus amyloliquefaciens ytk1 and its application in tobacco leaf curing

Examples

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Effect test

Embodiment 1

[0021] Example 1 Isolation and Screening of Thermostable Bacillus amyloliquefaciens YTK1

[0022] Separation and purification: Bring the fresh tobacco leaves picked from the test base of Nanxiong Tobacco Research Institute back to the laboratory, weigh 5 g of fresh tobacco leaf samples, wash them with distilled water, then cut the leaves into sterilized petri dishes, and use 3% of hydrogen peroxide (H 2 o 2 ) soaked for 4min (to remove the rotting material on the root surface), washed once with sterile water, and then washed with 0.1% mercury chloride (HgCl 2 ) soaked for 5 min, and vibrated in sterile water for 5-7 times, 5-10 min each time, and spread the last washing solution on the LB solid medium to test whether the disinfection was thorough. Tobacco leaf samples thoroughly sterilized on the surface were inoculated in 250 mL Erlenmeyer flasks with 80 ml LB liquid medium at 37 °C and 120 r / min for 2 days, and then placed in a constant temperature circulating water bath a...

Embodiment 2

[0027] Example 2 Identification of Bacillus amyloliquefaciens YTK1

[0028] The strain is rod-shaped and produces spores. The colony grew faster on the LB medium plate, and formed a bacterial film in the liquid medium, and the colony on the solid medium was pale yellow, opaque, with rough surface and irregular edges. 37 ℃, grow for 48 hours, colony diameter 8 ~ 12 mm. The pH growth range is wide, and it can tolerate pH 3-10. The optimum growth temperature is 37°C and the optimum growth pH value is 6-7. It is resistant to 1.5% NaCl, 300 μg / mL ampicillin, 100 μg / mL streptomycin and tetracycline, 5 μg / mL chloramphenicol and erythromycin; Toxin and neomycin are poorly tolerated, and low concentrations (5 μg / mL) of these antibiotics can inhibit their growth. Facultative anaerobic, able to utilize starch, Tween 40, Tween 80, L-arabinose, D-arabinose, sucrose, D-glucose, maltose, D-mannose, α-D-lactose, D -Galactose, sorbose, L-fructose, D-fructose, melibiose, gentiobiose, D-cell...

Embodiment 3

[0031] Example 3 Effect of Bacillus amyloliquefaciens YTK1 on degrading starch during tobacco leaf curing

[0032] The test strain YTK1 was expanded in the spore-optimized liquid medium for 48 h, and the supernatant bacterial suspension in 100 mL of the culture medium was taken to determine the OD 600 , and then dilute the bacterial suspension to OD 600 =1, transferred to a sterile watering can, and evenly sprayed on the surface of tobacco leaves (same batch, same variety, and same part) before curing, and this treatment was set as the test group. The control group sprayed the same amount of water on the tobacco leaves before curing. Then put it into the barn for roasting, take it out from the barn after the roasting, observe the color of the tobacco leaves and take pictures (such as figure 2 shown). The cured tobacco leaves were determined by the iodine colorimetric method to measure the starch content of the tobacco leaves. The results showed that the tobacco leaves of ...

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Abstract

The invention relates to the technical field of microorganisms, and specifically discloses a strain of thermoresistant Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) YTK1 and its application in tobacco leaf curing. The strain was deposited in the Guangdong Microbial Culture Collection Center (GDMCC) on May 6, 2016, and the strain preservation number is GDMCC No: 60033. The present invention finds that the strain YTK1 has strong environmental adaptability, can tolerate high temperature growth and grows well, and can efficiently degrade the starch content of tobacco leaves and improve the color and aroma of tobacco leaves. The cured tobacco leaves treated by the strain can fully degrade starch and can be used to prepare Tobacco leaves are improved by spraying fungicides.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of thermostable Bacillus amyloliquefaciens YTK1 and its application in tobacco leaf curing. Background technique [0002] Tobacco is an economic crop for leaves, and its production efficiency mainly depends on the quality of tobacco leaves. In recent years, with the popularization and application of new production technologies such as new variety breeding, intensive cultivation, and balanced fertilization, the level of flue-cured tobacco leaves in my country has increased, but there is still a certain gap with foreign high-quality tobacco leaves. Starch is an important basic organic compound in tobacco leaves. It generally contains about 25-40% in fresh tobacco leaves. Significant changes occur during the baking process. The degradation, conversion, consumption and accumulation of carbohydrates determine the internal quality and appearance of tobacco leaves. pros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B3/10C12R1/07
CPCA24B3/10C12N1/205C12R2001/07
Inventor 贺广生王勇张国华谭志远王行王军彭桂香
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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