Liquid medium used for production of laccase through fungal fermentation, and preparation method and application thereof

Abstract

The invention discloses a liquid medium used for production of laccase through fungal fermentation, and a preparation method and application thereof. Each L of the liquid medium comprises 10 to 40 g of shaddock peel powder, 5 to 10 g of wheat bran powder, 3 to 10 g of soybean meal, bean dregs or a mixture soybean meal and bean dregs, 3 to 10 g of ammonium chloride, 0.1 to 0.5 g of copper sulfate pentahydrate, 5 to 15 g of glucose, maltose, or a mixture of glucose and maltose, 5 to 15 g of sodium chloride, 1 to 2 g of potassium dihydrogen phosphate and 0.3 to 0.8 g of calcium chloride. The liquid medium provided by the invention uses agricultural by-products like shaddock peel as a main raw material and can substantially increase the laccase fermentation output of a plurality of fungi; and the medium has the characteristics of easiness in preparation, easy availability of raw materials, low price, etc., reduces industrial preparation cost for laccase and provides an approach for comprehensive utilization of shaddock peel.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a liquid culture medium for producing laccase by fungal fermentation, a preparation method and application thereof. Background technique [0002] Laccase (EC 1.10.3.2) is a blue polycopper oxidase, which was first discovered by Japanese scholars in 1883 in a Japanese lacquer tree. It is currently the only lignin-degrading enzyme that has achieved industrial production and application. In addition to plants, studies have found that laccases are also present in bacteria, fungi and insects. In particular, a class of white-rot fungus laccase, which has a corrupting effect on trees, has the highest yield of laccase, and is currently the most important laccase-producing strain. Laccase is a one-electron oxidase that can oxidize substrates while generating water using oxygen as an electron acceptor. [0003] Because laccase has low substrate specificity and has oxid...

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Problems solved by technology

[0005] Chinese patent CN 102719410 B provides a culture medium formula specially used for laccase and its preparation method. The culture medium used in this patent mainly contains ingredients such as sweet potato juice, mannose, yeast extract, peptone, casein and maltose. After 14 days of fermentation culture, the average activity of laccase in the fermentation broth is about 0.34U / mL, which has the characteristics of high cost of medium, long fermentation time and low enzyme production activity

[0006] Chinese patent CN 102399704 B provides a liquid medium suitable for the growth of white rot fungi and the production of laccase and its application. The medium used in this patent mainly contains bran, macroelements, trace elements and relatively expensive acid hydrolysis Compositions such as casein and vitamin B1, the activity of laccase in the fermentation broth after 13 days of cultivation using this medium is 50U / mL, which has the characteristics of complex production of medium, high cost and low fermentation efficiency

[0007] Chinese patent CN 102181410 B provides a method for producing laccase by fermentation. The medium used in this patent mainly contains glucose, ammonium tartrate, macroelements, trace elements and relatively expensive 2,2-azino-bis(3 -Ethyl-benzothiazole-6-sulfonic acid) diammonium salt and vitamin B1 and other ingredients, the use of the hairy hairy bacteria to reach 56.45U / mL after 6 days of fermentation, has the characteristics of complex production of culture medium and high cost

The pomelo peel accounts for about 40% to 50% of the weight of the whole pomelo fruit. Generally, the pomelo pulp is directly treated as waste after processing or eating, resulting in a great waste of resources, and the discarded pomelo peel is harmful to the environment due to mold and odor. Pollution

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