Microorganism feed additive capable of protecting pig livers and preparation method of microorganism feed additive

A microbial feed and additive technology, applied in the field of microbial feed additives for protecting pig liver and its preparation, can solve the problems of physiological and pathological burden on the liver, and achieve the effects of promoting drug and cell metabolism, improving liver function, and protecting the liver

Inactive Publication Date: 2017-02-15
上海绿博生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the physiological and pathological burden caused by environmental change stress and disease also puts a lot of pressure on the liver

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The invention provides a preparation method of a microbial feed additive for protecting pig liver, comprising the following steps:

[0037] Step a. Bacterial strain screening: the strains are from healthy pig small intestine, lactic acid bacteria are isolated by using lactic acid bacteria medium, and Lactobacillus plantarum and Bacillus subtilis are screened; Saccharomyces cerevisiae is isolated from the beer fermentation broth with McFarland agar medium;

[0038] Step b. Inoculate the isolated Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae respectively in the broth medium, add 0.1-1% sodium selenite at the same time, and cultivate at 28-35°C for 20-25 hours, then Saccharomyces cerevisiae was inoculated in McFarland agar medium, Bacillus subtilis was inoculated in nutrient agar medium, Lactobacillus plantarum was inoculated in lactic acid bacteria medium, and streaked culture was carried out, and the fast-growing and obvious bacterial characteris...

Embodiment 1

[0046] Step 1: Strain screening and seed production

[0047] a. Bacterial strain screening: take chyme from the small intestine of healthy goose as the bacterial source, use the lactic acid bacteria culture medium of MRS medium to isolate lactic acid bacteria, screen Lactobacillus plantarum and Bacillus subtilis; use PDA agar medium to isolate in beer fermentation broth Saccharomyces cerevisiae is kept as a strain in the laboratory, and the following strains are used in the present invention: Lactobacillus plantarum (ATCC8014), Saccharomyces cerevisiae (IFO0203), Bacillus subtilis (ACCC10623);

[0048] b. Inoculate the isolated Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae respectively in the broth medium, add 0.5% sodium selenite at the same time, cultivate at 30°C for 24 hours, and then inoculate the Saccharomyces cerevisiae Inoculate in the Meclary (Meclary) agar medium composed of 1g of glucose, 1.8g of potassium chloride, 2.5g of yeast extract, 8...

Embodiment 2

[0062] The first step: bacterial classification screening and production seed production are identical with embodiment 1;

[0063] The second step: the preparation of compound bacterial agent

[0064] a. Weigh each production strain in the eggplant bottle according to the following weight ratio: 8% of Lactobacillus plantarum, 80% of Saccharomyces cerevisiae, and 12% of Bacillus subtilis.

[0065] b. Mix the production strains, add 5% molasses, 0.6% peptone, 3% glucose, 0.8% sodium chloride, 2.5% yeast extract, 0.08% magnesium sulfate, and 88.02% water according to the proportion of 4% of the weight of the culture medium. In the composite culture medium composed of bacteria, adjust the pH value to 7.0, put it into the container and heat it to 121°C for sterilization for 15 minutes, cool it to 30°C and ferment in the fermenter for 8 hours, the ventilation rate is 25L / hour, the stirring speed 180rpm;

[0066] The third step: the expansion and domestication of compound bacterial...

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Abstract

The invention relates to a microorganism feed additive capable of protecting pig livers and a preparation method of the microorganism feed additive. All strains adopted by the microorganism feed additive are screened out from intestinal tracts of animals, and a strain compound culturing method is adopted, so that an antagonism mechanism of the strains is avoided, and fermented products are optimized; and in the whole production technology, beneficial microorganisms are furthest maximized, and a main fermented product namely glutathione is wholly recovered from intracellular and extracellular positions. According to the microorganism feed additive and the preparation method thereof disclosed by the invention, oxygen free radicals can be effectively eliminated, so that the stability of liver cell membranes is improved, the activity of liver enzymes is promoted, the absorption of iron can also be promoted, the integrity of red cell membranes can be maintained, the biosynthesis of DNA is maintained, the normal growth of cells is maintained, and cell immunity is maintained; as a micro-ecologic preparation, the microorganism feed additive can effectively promote the digestion and the absorption of the feed by the animals, the lean meat percentage is increased, toxin disoperation can be alleviated, the feed conversion rate is increased, and the production capacity of bred animals is improved.

Description

technical field [0001] The invention relates to the technical field of feed additive preparation, in particular to a microbial feed additive for protecting pig liver and a preparation method thereof. Background technique [0002] The liver is the largest gland in the pig body. Many substances produced by liver cells can be directly released into the blood to affect and regulate the body's metabolism and physiological activities. In addition, the liver also has a duct system, and the bile produced can be discharged into the duodenum through the bile duct. Therefore, the liver has the properties of an endocrine gland and an exocrine gland. The liver's rich blood supply and unique ecological structure make its metabolism extremely active. It is not only closely related to various tissues and organs in the metabolism of sugar, fat, protein, vitamins and hormones, but also has important functions such as secretion, excretion and biotransformation. [0003] However, with the rap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/20A23K50/30C12N1/18C12N1/20C12R1/125C12R1/25C12R1/865
CPCC12N1/18C12N1/20A23V2400/169
Inventor 张美英张玉辉
Owner 上海绿博生物科技发展有限公司
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