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Ginseng bacterium TY15-2 with effects of disease prevention and growth promotion and application thereof

A technology for ginseng rust bacteria and ginseng, which is applied in the field of microorganisms, can solve the problems of quality degradation, environmental pollution pathogen resistance, unsatisfactory control effect, etc., and achieves the effect of increasing fresh weight

Inactive Publication Date: 2017-02-22
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of disease prevention and control is to use chemical pesticides to control the disease occurrence period, but the control effect on perennial underground root diseases such as ginseng rust rot is not ideal, and long-term use of chemical pesticides or unreasonable use will also lead to pesticide residues in ginseng A series of problems such as exceeding the standard, declining quality, polluting the environment, and pathogenic bacteria developing drug resistance

Method used

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  • Ginseng bacterium TY15-2 with effects of disease prevention and growth promotion and application thereof
  • Ginseng bacterium TY15-2 with effects of disease prevention and growth promotion and application thereof
  • Ginseng bacterium TY15-2 with effects of disease prevention and growth promotion and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Screening of bacterial strain TY15-2

[0017] Healthy ginseng roots were collected in Tonghua City, Jilin Province in October 2015. Screening of ginseng antagonistic strains was divided into primary screening and secondary screening. In the preliminary screening, 120 strains of endophytic bacteria and 178 strains of rhizobacteria were screened for antibacterial activity by the mixed bacteria method, and 1ml sterile water was added to ginseng rust rot bacteria ( Cylindrocarpon destructans ), Botrytis ginseng ( Botrytis cinerea ), Sclerotinia ginseng ( Sclerotinia schinseng ), Phytophthora ginseng ( Phytophthora cactorum ), Panax ginseng ( Alternaria panax ), ginseng root rot fungus ( Fusarium solani ), Panax ginseng ( Rhizoctonia solani ) In the PDA culture test tubes of 7 kinds of ginseng pathogenic bacteria, the mycelium and spores were scraped off with an inoculation needle, and the bacterial suspension was mixed with the PDA medium, and then p...

Embodiment 2

[0018] Identification of embodiment 2 bacterial strain TY15-2

[0019] Streak the TY15-2 strain on the NA medium, and culture it at 28°C to observe that the strain TY15-2 on the NA medium shows a light yellow opaque circular small colony with neat edges, such as figure 2 shown.

[0020] The TY15-2 strain cultured in NA medium was taken to prepare samples by negative staining, and it was observed under a transmission electron microscope that the bacteria were rod-shaped and had perinatal flagella, such as image 3 shown.

[0021] The physiological and biochemical characteristics of the TY15-2 strain were determined by referring to some culture media (Table 1) and methods recommended in the "Common Bacterial System Identification Manual" and "Berger System Identification Manual". It was found that the strain TY15-2 was Gram-negative bacteria ; Catalase, oxidase, V-P reaction is positive; can use citric acid, malonic acid to reduce nitrate; but can not hydrolyze starch, but ca...

Embodiment 3

[0024] Example 3 Determination of growth-promoting properties of bacterial strain TY15-2

[0025] 1. Determination of Nitrogen Fixation Capacity

[0026] Strain TY15-2 was streak-inoculated on Ashby solid medium, and cultured at 30°C to observe the growth of the strain. It was found that the strain TY15-2 grew well under nitrogen-free conditions and had nitrogen-fixing potential, such as Figure 5 shown.

[0027] 2. Determination of Potassium Solubilizing Ability

[0028] Inoculate the strain TY15-2 on a silicate solid medium and culture at 30°C to observe the size of the potassium solution circle, as shown in Figure 5 As shown, the strain TY15-2 has obvious potassium-dissolving circles; then, the strain TY15-2 was inoculated on the LB medium by flame photometry, and the absorbance value was measured after culturing for 12 hours. Quantitative measurement of its potassium content by flame photometer can reach 7.6 μg / mL.

[0029] 3. Determination of the ability to produce I...

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Abstract

The invention discloses P. agglomerans TY15-2. The bacterial strain TY15-2 has the characteristics of nitrogen fixation, potassium dissolving, auxin generation and siderophore generation, and has a promotion effect on ginseng growth. The bacterial strain TY15-2 is identified as P. agglomerans. Nitrogen fixation potential is achieved, potassium dissolving capacity can reach 7.6 micrograms per milliliter, IAA generation capacity is high and can reach 6.25 micrograms per milliliter, the siderophore generation capacity is achieved, and the activity reaches 84.6%. A good effect is achieved for prevention and control of diseases at the seedling stage of ginseng and prevention and control of the rusted root disease at the adult plant stage. An obvious promotion effect is achieved for ginseng root growth, and the fresh weight is increased by 10.64%. Discovery of the P. agglomerans lays a good foundation for research and application of ginseng biological prevention and control.

Description

technical field [0001] The invention belongs to the field of microbes, specifically ginseng disease-preventing and growth-promoting bacteria TY15-2 and its application. Background technique [0002] Biological control has the advantages of long-lasting effect, multiple functions of disease prevention and growth promotion, no residue, and environmental friendliness. It has great development potential for effective control of root diseases. The prerequisite for the development of biological control is to obtain beneficial microorganisms with high-efficiency biological activity functions. Among them, plant endophytes are a type of microorganisms that colonize in plants and establish a harmonious relationship with plants. Some strains have biological functions such as antagonistic and growth-promoting. , can produce a variety of biologically active substances, especially in the aspect of new plant anti-pest substances, which provides us with a new class of microbial resources. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 陈长卿姜云高洁任英英褚逸轩
Owner JILIN AGRICULTURAL UNIV
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