A biosynthetic gene cluster of pactamide and its application

A technology of pactamide and biosynthesis, applied in the direction of plant genetic improvement, application, microorganism, etc.
CN106434702BActive Publication Date: 2019-06-21SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2019-06-21

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Abstract

The invention discloses a biosynthetic gene cluster of paquete amide and an application thereof. The biosynthetic gene cluster of paquete amide comes from (Streptomyces pactum)SCSIO 02999, and the cluster comprises five genes including heterozygous polyketone / nonribosomal peptide synthetases genes ptmA, FAD dependent redox enzyme genes ptmB1, phytoene dehydrogenase genes ptmB2, cyclase genes ptmC and hydroxylase genes ptmD. According to the biosynthetic gene cluster of paquete amide and the application thereof, information in genes and proteins related to biosynthesis of the paquete amide provides theoretical foundations and materials for conducting genetic modification on the biosynthesis of multi-ring tetramate macrocylic lactam family. By conducting genetic modifications on the biological synthetic genes, 6 new structures antineoplastic paquete amide compounds pactamide A-F are obtained, and thus effective compound entities are provided for research and development of antineoplastic drugs.
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Description

Technical field

[0001] The invention belongs to the field of microbial genetic engineering, and specifically relates to a biosynthetic gene cluster of Pacotamide and its application. Background technique

[0002] Synthetic biology technology, which has developed vigorously in recent years, has brought new opportunities for activating the silent biosynthetic gene clusters contained in deep-sea microorganisms and discovering new anti-tumor lead compounds. On the basis of clarifying natural biosynthetic pathways and understanding the mechanism of combinatorial biosynthesis, using combinatorial biosynthesis technology to knock out and recombine the biosynthetic genes of antibiotics in vivo can not only produce structural analogs of "non-natural" natural products. It can also increase the yield of natural products, or directional accumulation of required natural products, and provide diversification of compound structure and biological activity for natural product discovery and drug d...

Claims

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