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Method for induced differentiation of human placenta sub-totipotent stem cells to obtain cardiac muscle cells and application thereof

A technology of subtotipotent stem cells and cardiomyocytes, which is applied in the field of inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes, which can solve the problems of difficult recovery and limited proliferation ability

Inactive Publication Date: 2017-05-24
广东科门生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current therapeutic approaches for heart failure focus on early angiogenesis and inhibiting further loss of cardiomyocytes, a terminally differentiated cell with extremely limited proliferative capacity and extremely difficult to recover from loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for inducing the differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.

[0015] Stem cell culture medium includes: DMEM medium, hydroxyethyl starch, human serum albumin, potassium bicarbonate, diethylamine tetraacetic acid sodium salt, sodium selenite, transferrin, hyaluronic acid, taurine, placental growth factor, fibroblast factor, glutathione, lipoic acid, astragalus polysaccharide, codonopsis polysaccharide, jujube polysaccharide. Wherein the concentration of hydroxyethyl starch is 3.2g / L, the concentration of human serum albumin i...

Embodiment 2

[0018] A method for inducing the differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.

[0019] Stem cell culture medium includes: DMEM medium, hydroxyethyl starch, human serum albumin, potassium bicarbonate, diethylamine tetraacetic acid sodium salt, sodium selenite, transferrin, hyaluronic acid, taurine, placental growth factor, fibroblast factor, glutathione, lipoic acid, astragalus polysaccharide, codonopsis polysaccharide, jujube polysaccharide. Wherein the concentration of hydroxyethyl starch is 3g / L, the concentration of human serum albumin is ...

Embodiment 3

[0022] A method for inducing the differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.

[0023] Stem cell culture medium includes: DMEM medium, hydroxyethyl starch, human serum albumin, potassium bicarbonate, diethylamine tetraacetic acid sodium salt, sodium selenite, transferrin, hyaluronic acid, taurine, placental growth factor, fibroblast factor, glutathione, lipoic acid, astragalus polysaccharide, codonopsis polysaccharide, jujube polysaccharide. The concentration of hydroxyethyl starch is 3.5g / L, the concentration of human serum albumin is 1.8g / L...

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PUM

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Abstract

The invention discloses a method for induced differentiation of human placenta sub-totipotent stem cells to obtain cardiac muscle cells. The method comprises the following steps: inoculating human placenta sub-totipotent stem cells into a 24-pore plate containing a stem cell culture medium; after cell attachment, removing the stem cell culture medium; and adding a cardiac muscle cell culture medium for culture for 28 days, and changing the culture medium every 3 days. The stem cell culture medium comprises a DMEM culture medium, hydroxyethyl starch, human serum albumin, potassium bicarbonate, ethylenediamine tetraacetic acid sodium salt, sodium selenite, transferrin, hyaluronic acid, taurine, placental growth factors, fibroblast growth factors, glutathione, lipoic acid, astragalus polysaccharide, codonopsis pilosula polysaccharide and jujube polysaccharide. The cardiac muscle cell culture medium comprises an RPMI-1640 culture medium, trehalose, taurine, fibroblast growth factors, endothelial cell growth factors, potassium bicarbonate, amino acid chelated selenium, glutamine, progesterone, coenzyme I, lipoic acid, salvianolic acid B, polygonatum polysaccharide and ginsenoside.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes and an application thereof. Background technique [0002] Heart failure caused by cardiomyocyte dysfunction is a major disease worldwide, and the final cause of death in heart failure patients is mainly loss of cardiac pumping function or arrhythmia. The one-year mortality rate of patients with severe heart failure exceeds 50%. Although heart transplantation is possible for terminally ill patients, about 20% of patients die while waiting for organ transplantation due to lack of donor organs. Due to the high morbidity and mortality of heart failure, the death of transplanted hearts, complications such as immune rejection, and the late failure of transplanted hearts, it is urgent to develop new therapeutic methods to improve the function of cardiomyocytes and prevent the occur...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61L27/38
CPCC12N5/0657A61L27/3804A61L2430/20C12N2500/05C12N2500/12C12N2500/24C12N2500/30C12N2500/33C12N2501/10C12N2501/113C12N2501/115C12N2501/119C12N2501/70C12N2501/90C12N2501/905C12N2501/998C12N2506/025
Inventor 张正亮王峻
Owner 广东科门生物科技有限公司
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