Human-derived adipose tissue-derived stem cell factor, preparation method and application thereof

A technology of mesenchymal stem cells and cytokines, which is applied in the field of human adipose-derived mesenchymal stem cell factors and their preparation, can solve the problems that the effects need to be further improved, achieve good market prospects, accelerate the growth of skin collagen cells, and increase collagen secretion.

Active Publication Date: 2017-05-24
中科领康(广州)医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Human-derived adipose tissue-derived stem cell factor, preparation method and application thereof
  • Human-derived adipose tissue-derived stem cell factor, preparation method and application thereof
  • Human-derived adipose tissue-derived stem cell factor, preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0029] Embodiment 1 Preparation of adipose-derived mesenchymal stem cell factor of the present invention

[0030] 1. Source of fat

[0031] The donor signed the informed consent form for fat donation, and the donor's coagulation function, hepatitis C virus antibody, HIV Ⅰ / Ⅰ antibody, hepatitis B virus antigen and Treponema pallidum antibody were checked before collection. Separation of fat to ensure that the collected fat is free of virus contamination.

[0032] 2. Preparation method

[0033] Fat-improved medium and hypoxic culture group of the present invention:

[0034] 1. Obtaining the supernatant of human adipose-derived mesenchymal stem cells (AADSC) hypoxic culture

[0035] (1) Cell culture:

[0036] Use a straw to suck out the liquid in the lower layer of the collection bottle, leaving the yellow fat tissue, and be careful not to suck the yellow fat particles;

[0037] Add 30ml of normal saline, shake the collection bottle, wash the adipose tissue, and then let it ...

Embodiment 2

[0076] Embodiment 2 The preparation of mesenchymal stem cell factor of the present invention

[0077] 1. Take the cytokine concentrate prepared in Example 1, and adjust the concentration of the cytokine concentrate through repeated ultrafiltration, so that the EGF content in the concentrate is 6ug / ml.

[0078] 2. Add a certain amount of lyoprotectant to the cytokine concentrate to make the EGF content 0.5ug / ml, and adjust the pH value to 6.0.

[0079] Among them, the lyoprotectant contains the following components per 100ml: tocopherol 0.5g, glutathione 0.4g, human albumin 0.5ml, dextran 3.5g, trehalose 1.5g, mannitol 4g, hydroxyproline Acid 0.05g, Arginine 0.02g, Histidine 0.2g, Methionine 0.2g, Tyrosine 0.2g, Glycine 2g, Sodium Citrate 0.26g. The freeze-drying conditions are as follows: the temperature is at -25-40° C., the pressure is maintained at 10-30 Pa, and the time is 18-28 hours to obtain the cytokine freeze-dried powder.

[0080] 3. Activity determination of cytok...

Embodiment 3

[0084] Embodiment 3 The preparation of cytokine cosmetic or medicine of the present invention

[0085] Take the freeze-dried cytokine powder prepared in Example 2, add biopolysaccharide glue, collagen, folic acid, and sterile ultrapure water to make a composition, which can be added to conventional skin care products or used alone as a skin care product .

[0086] Wherein 10ml of the aqueous solution contains 0.05g of cytokine freeze-dried powder, 0.1g of biological polysaccharide glue, 0.3ml of collagen sterile solution and 0.05g of folic acid.

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Abstract

The invention discloses a human-derived adipose tissue-derived stem cell factor, a preparation method and application thereof, and particularly discloses a method for culturing a human-derived adipose tissue-derived stem cell factor. The method comprises the following steps: culturing human adipose tissue-derived stem cells in a high-coenzyme serum-free culture medium until the cell confluence is 75-85 percent, and collecting supernatant. The invention further discloses a method for preparing human-derived adipose tissue-derived stem cell factor concentrated solution, the concentrated solution prepared by the method, further application of the concentrated solution, a cell factor concentrate prepared by the method, cosmetics and medicines prepared from the cell factor concentrate. The concentrated solution prepared by the method has high content of adipose tissue-derived stem cell factors and stable quality, and a product prepared by combining the concentrated solution with other materials in a specific ratio has a good effect of repairing skin injury; and the method is simple and feasible, is suitable for large-scale production, and has excellent market prospect.

Description

technical field [0001] The invention belongs to the technical field of skin restoration, and in particular relates to a human-derived adipose-derived mesenchymal stem cell factor and its preparation method and application. Background technique [0002] In recent years, the factors secreted by mesenchymal stem cells have become a research hotspot. These cytokines can effectively regulate the body's cell signal transduction, activate human stem cells, and then physiologically repair or replace damaged, diseased and aging cells in the body. Adding cytokines to beauty cosmetics not only has the moisturizing and whitening effect of general cosmetics, but also can repair damaged skin, eliminate skin wrinkles, shrink pores, improve complexion, etc., and has attracted more and more attention. However, cytokine cosmetics on the market are generally manufactured by directly blending or freeze-drying into the cosmetic matrix. The content of cytokines is low and contains a lot of sugar ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C07K14/52C07K1/34C07K1/14A61K8/98A61K8/64A61K35/28A61Q19/00A61Q19/02A61Q19/08A61P17/00
CPCA61K8/64A61K8/981A61K35/28A61Q19/00A61Q19/02A61Q19/08C07K14/52C12N5/0667C12N2500/40C12N2500/90C12N2501/70
Inventor 赵明建钟伟兴
Owner 中科领康(广州)医疗有限公司
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