Recombinant microorganism for producing cytidine and method for producing cytidine

A technology of recombinant microorganism and cytidine, applied in the field of cytidine production, can solve the problems of unknown strain genotype, low cytidine yield, poor repeatability, etc., and achieve the effect of high popularization and application value.
CN106754602AActive Publication Date: 2017-05-31BIOSYNTHETICA INC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BIOSYNTHETICA INC
Publication Date
2017-05-31

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Abstract

The invention provides a recombinant microorganism for producing cytidine and a method for producing the cytidine from the recombinant microorganism. A degradation and use gene of the cytidine is knocked off, and the gene encodes cytidine ammonialyase, ribonucleotide hydrolase, cytidine / uridine kinase and nucleoside transporter. Meanwhile key enzymes in biological synthesis process of the cytidine are over-expressed, including degrading cytidine triphosphoric acid to cytidine triphosphoric acid pyrophosphorylase of the cytidine monohosphoric acid, and catalyzing the cytidine monohosphoric acid to cytidine monohosphoric acid phosphorylase of the cytidine. In addition, pyrimidine nucleoside process is subjected to genetic engineering reform, and feedback inhibition of the synthesis process is relieved. By using a biological fermentation method, the cytidine yield greater than 20g / L can be achieved for a recombinant strain in a fermentation tank of 5L, industrial on-scale production can be achieved, and meanwhile the recombinant microorganism is low in cytidine production cost, small in pollution, green and environmental-friendly and relatively high in popularization and application value.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant microorganism producing cytidine, producing cytidine by using the recombinant microorganism and producing cytidine by using the recombinant microorganism through a biological method. Background technique

[0002] Cytidine is a kind of nucleoside, which is synthesized from cytosine and ribose, and the two are connected by β-N1-glycosidic bonds. White or off-white crystal or powder; soluble in water, soluble in acid and alkali, slightly soluble in ethanol, insoluble in organic solvents. Molecular formula C 9 h 13 N 3 o 5 , The relative molecular mass is 243.22, the melting point is 210.0~220.0, and the specific rotation is +31~+35°. It is mainly used in the preparation of cytidine triphosphate (CTP), citicholine (sodium salt) (Citicholine, CDP-choline), 2′, 3′-dideoxycytidine and cytarabine and other raw materials.

[0003] At present, the production of ...

Claims

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