A kind of stem cell culture fluid and injection

Abstract

A stem cell culture solution is prepared from raw materials as follows: 10% of a compound amino acid injection, 1% of water-soluble vitamins for injection, 0.5% of glutamine dipeptide, 10% of human albumin, 10% of a hydrolyzed protein injection, 0.5% of a microelement injection and 68% of a plasmalyte-A solution, wherein the pH value is 6.8-7.0, and the osmotic pressure is 310 mOsm / kg. The stem cell culture solution is used for culturing mesenchymal stem cells, and a mesenchymal stem cell culture supernatant is collected; the collected culture supernatant is filtered by a filter membrane, and filtrate is collected and is the stem cell injection. A decellularized stem cell secretion factor is used for intravenous injection, so that the dispute of stem cells in the aspect of ethic is effectively avoided; the stem cell culture solution is prepared from clinical raw materials, is safe and reliable, provides nutrition for the living environment of the stem cells, can effectively promote the stem cells to secrete a large quantity of cell factors and keeps the activity of the stem cells; through cooperation of a nutritional supplement formula and the stem cell secretion factor, the sub-health state of a body can be effectively relieved, and the body health is regained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mesenchymal stem cell culture solution and an injection. Background technique [0002] Mesenchymal stem cells (Mesenchymal stem cells) are a type of adult stem cells with multi-directional differentiation potential. obtained from various organizations. Mesenchymal stem cells are non-terminal differentiated cells, which have both the characteristics of mesenchymal cells and the characteristics of endothelial cells and epithelial cells; as a type of pluripotent stem cells, they can develop into bone, Cartilage, muscle, tendon, liver, fat, nerve, endothelial and islet-like cells proliferate and differentiate. [0003] During the culture process of mesenchymal stem cells, stem cell factor (SCF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), platelet-derived growth factor A (PDGF) will be secreted. -A), tr...

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Problems solved by technology

[0006] Patent CN102920735A and patent CN1966080A both provide a mesenchymal stem cell injection, but the injection contains live cells, which reduces the safety and controllability of clinical use. Bovine serum, the introduction of exogenous protein, is more risky to use; patent CN1618981A provides a method for preparing stem cell secreted factors, the stem cells become immortal cells after genetic modification, because there are certain potential risks in genetic modification, and stem cells secrete The factors are separated and purified, the process is complicated, and the secreted factors of stem cells will inevitably cause losses; patent CN102008507A provides an umbilical cord mesenchymal stem cell injection, but it only adds human serum albumin and heparin calcium, and the formula has a single nutritional structure, which is harmful to cells. The content of activity and secreted factors will be affected to a certain extent, and the injection contains living stem cells, so there are also problems in safety; patent CN102703385A provides a mesenchymal stem cell culture medium, which uses the cell culture medium to add serum-free additives, bFGF , EGF, fetuin and antibiotics to make stem cell culture fluid. First, the cell culture fluid is not a clinical grade reagent and cannot be directly applied to the human body. Secondly, there is a risk of allergies if antibiotics are added to the culture fluid. Finally, its nutritional structure is single. It cannot meet the needs of cell nutrition, which may have a certain impact on cell activity and secreted factor content

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