Check patentability & draft patents in minutes with Patsnap Eureka AI!

Test piece for quick test of bacillus cereus and preparation method and test method of test piece

A technology of Bacillus cereus and test strips, which is applied in the field of rapid detection, can solve problems such as blurring of colony edges and influence on result interpretation, and achieve uniform distribution, avoid inaccurate counting results, and clear color development

Pending Publication Date: 2017-06-09
贵州勤邦食品安全科学技术有限公司
View PDF21 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the medium carrier of the existing test piece in the market is non-woven fabric, which has the disadvantage that the target colony diffuses and grows on the non-woven fabric, making the edge of the colony blurred, which affects the interpretation of the results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Test piece for quick test of bacillus cereus and preparation method and test method of test piece
  • Test piece for quick test of bacillus cereus and preparation method and test method of test piece
  • Test piece for quick test of bacillus cereus and preparation method and test method of test piece

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Rapidly detects the composition of the test piece of bacillus cereus

[0034] A test piece for rapid detection of Bacillus cereus, comprising a lower bottom plate 4, pearl paper 2, an upper film assembly 1, a test piece label 5 and a characteristic chromogenic liquid culture medium for Bacillus cereus. The lower bottom plate 4, the pearl paper 2 and the upper film assembly 1 are rectangular, and there is a circular groove 3 in the middle of the pearl paper 2, and the characteristic chromogenic liquid culture medium of Bacillus cereus is evenly distributed in the circular groove 3. Each 100mL Bacillus cereus characteristic chromogenic liquid medium contains tryptone, beef extract powder, sodium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, cold water soluble gel, bacteriostatic agent mixture, chromogenic enzyme hydrolysis substrate Mixture. The enzymatic hydrolysis substrate mixture consists of 5-bromo-6-chloro-3-indooctyl ester,...

Embodiment 2

[0043] The preparation of the test piece of embodiment 2 rapid detection bacillus cereus

[0044] 2.1 Preparation of characteristic chromogenic liquid medium for Bacillus cereus

[0045] Among them, the preparation method of 100mL Bacillus cereus characteristic chromogenic liquid medium: Weigh 1.2g of tryptone, 0.15g of beef extract powder, 0.3g of sodium chloride, 0.25g of potassium dihydrogen phosphate, 0.25g of dipotassium hydrogen phosphate, cold water Soluble glue 0.9g, add 100mL of distilled water to a beaker and stir to dissolve, sterilize at 121°C for 15 minutes in an autoclave, cool to room temperature, add 20000IU of filter-sterilized bacteriostatic agent mixture and chromogenic enzymatic hydrolysis substrate in sequence 0.020 g of the mixture was used to obtain the characteristic chromogenic liquid culture medium of Bacillus cereus, which was refrigerated at 0-4°C for later use.

[0046] 2.2 Pretreatment of each component of the test piece:

[0047] 2.2.1 Upper me...

Embodiment 3

[0052] The detection of Bacillus cereus in the sample to be tested in embodiment 3

[0053] When using the rapid detection Bacillus cereus test piece of the present invention to detect Bacillus cereus in a sample, the following steps are mainly included:

[0054] First sample processing: take 25mL (g) of the sample and put it into a sampler or a homogenizing cup containing 225mL of sterile diluent (sterilized phosphate buffer saline or saline) to make a 1:10 sample homogeneous solution, and use 1mL Take 1mL of 1:10 homogeneous sample solution with a sterile pipette, inject it into a test tube containing 9mL of sterile diluent, shake well to obtain a 1:100 sample dilution solution, and dilute sequentially according to the above operation steps to prepare a 10-fold incremental series The sample dilution homogeneous solution. Generally, 2 to 3 dilutions are selected for testing, and liquid samples with a small amount of bacteria (such as drinking pure water and mineral water, et...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a test piece for quick test of bacillus cereus. The test piece comprises a lower substrate, pearl paper, an upper thin film component and a bacillus cereus characteristic chromogenic fluid medium. The middle of the pearl paper is provided with a circular groove, and the bacillus cereus characteristic chromogenic fluid medium is uniformly distributed in the circular groove. The test piece for quick test of the bacillus cereus has advantages of easiness in distinguishing of target bacteria, chromogenic clearness, simplicity in result determination, reliability in test result, short test time, simplicity and convenience in operation, low cost and the like and can play a significant role in microbiological detection.

Description

technical field [0001] The invention belongs to the field of rapid detection, relates to microorganism detection technology, in particular to a test piece for rapid detection of Bacillus cereus, a preparation method and a detection method of the test piece. Background technique [0002] Bacillus cereus (Bacillus cereus) is widely distributed in nature, and its detection rate in various foods is also high. Bacillus cereus or residual spores in food can grow and multiply, and food poisoning incidents will occur due to the heat-stable toxin produced by Bacillus cereus. The incidence of Bacillus cereus poisoning is relatively high, generally reaching 60% to 100%. The General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China passed the "Guidelines on the Microbiological Content of Food" in 2014, pointing out that each gram of ready-to-eat food contains more than 100,000 Bacillus cereus, which is not suitable for human consumption ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/04C12R1/085
CPCC12Q1/045
Inventor 何方洋冯才伟湛剑龙扶胜侯璐伟周绘贾玲玲付浩席芸芸易重任谢体波李平赵正苗朱亮张鹤
Owner 贵州勤邦食品安全科学技术有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com