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A kind of preparation method of dried specimen of gastrointestinal digestive system by rosin method

The technology of a digestive system and a production method is applied in the field of the production of dried specimens of the gastrointestinal digestive system by the rosin method, which can solve the problems of large consumption of glycerin, distorted color of the stomach and intestines, long cycle, etc. The effect of not damaging the inherent shape of the material and the effect of short time

Active Publication Date: 2020-07-10
SHENYANG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems existing in the above-mentioned prior art, and provides a method for preparing dried specimens of the gastrointestinal digestive system using the rosin method, which solves the problem of large consumption of glycerin, high cost, cumbersome steps and long cycle, and the problem of glycerin and turpentine in the prior art. Gastrointestinal color distortion caused by high-intensity permeabilization, easy to break, not suitable for making dried specimens of the gastrointestinal digestive system of medium and large animals, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Dried Rosin Specimen of Sheep Gastrointestinal Digestive System

[0033] 1. Select healthy adult small-tailed Han sheep corpses that died naturally, dissect them immediately, and take out the digestive system intact. Remove the omentum and mesentery, trim the surface fat of the large intestine and small intestine, and preserve the esophagus, rumen, reticulum, omasum, abomasum, gallbladder, pancreas, small intestine, large intestine, anus, etc.

[0034] Cut a 5 cm opening at the lesser curvature of the abomasum, wash the contents of the four gastric chambers and intestines with running water, ensure the integrity of the digestive tract from the throat to the anus, remove the liver and keep the gallbladder, and use a 5ml syringe to pierce along the cystic duct After inhaling and sucking out the bile, inflate the gallbladder and ligate the cystic duct, and soak it in a plastic basin for later use. The whole procedure should be completed within 4 hours to pre...

Embodiment 2

[0043] Preparation of Dried Rosin Specimen of Canine Gastrointestinal Digestive System

[0044] 1. Select the corpse of a healthy adult small hybrid dog that died naturally, dissect it immediately, take out the digestive system intact and without injury, keep the tongue, esophagus, stomach, gallbladder, pancreas, small intestine, large intestine, anus, and mesentery, and insert the funnel into the throat of the material. Wash the contents of the stomach and intestines with running water to ensure the integrity of the digestive tract, and immerse them in a plastic basin for later use.

[0045] 2. According to the antiseptic fixative formula: 100ml of commercially available formaldehyde + 870ml of water + 30ml of glacial acetic acid, the configuration is 10L. Tighten the end of the rectum with a thread, and pour the antiseptic fixative into the digestive tract with a funnel at the esophagus, so that the specimen is in a semi-filled state, tie the front end of the esophagus tight...

Embodiment 3

[0053] Making Rosin Dried Specimen of Chicken Gastrointestinal Digestive System

[0054] 1. Select the carcasses of healthy Hailan brown-shell hens that died naturally, dissect them immediately, take out the digestive system intact, and keep the hyoid bone, esophagus, crop, glandular stomach, gizzard, gallbladder, pancreas, small intestine, ileum, and cecum , cloaca, etc., to ensure the integrity of the digestive tract. Insert the funnel into the esophagus of the material, wash the content of the digestive canal with running water, and soak it in a water basin for later use.

[0055] 2. Antiseptic fixative (1L volume): 100ml formaldehyde + 870ml tap water + 30ml glacial acetic acid. Configure 6L antiseptic fixative. Tie the end of the rectum tightly with a thread, and pour the antiseptic fixative into the digestive tract with a funnel at the esophagus, so that the specimen is in a semi-filled state, tie the front end of the esophagus tightly with a thread, and place the spec...

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Abstract

The invention discloses a production method of dried specimens of gastrointestinal digestion systems through a rosin method. The method comprises the steps of taking materials, carrying out antiseptic fixation, carrying out ethanol dehydration fixation, carrying out gradient ethanol dehydration, soaking the materials in cell filling and sealing liquid, curing and shaping, brushing paint and coating. The rosin component in the cell filling and sealing liquid is rapidly filled into completely dehydrated gastrointestinal tissue cells in short time according to the principle of the dissolution in the similar material structure; meanwhile, the curing and shaping process is accelerated due to volatile characteristics of ethanol and turpentine; then the materials are brushed with paint, coated and rapidly dried to produce specimens of gastrointestinal digestion systems. The core of the technical scheme of the dried gastrointestinal specimens is application of rosin; the rosin is non-toxic and free of corrosion and has the characteristics of corrosion resistance, moisture resistance, mildew resistance and softness, so that the specimens has good effects of expelling parasite, resisting corrosion and resisting moisture; meanwhile, the specimens have light amber color, is rich in elasticity, has collapse rate being lower than 9% and can be preserved for a long time; the method is low in requirements on devices and reagents, few in steps and short in time, and is especially applicable to production of specimens of gastrointestinal digestion systems of medium and large-sized animals.

Description

technical field [0001] The invention relates to a preparation method of an animal digestive system specimen, in particular to a preparation method of a rosin method gastrointestinal digestive system dried specimen. Background technique [0002] Existing techniques for making specimens of the digestive system of animals usually adopt three production methods: soaking in formalin, drying in turpentine, and plasticizing polymers. For example, the Chinese invention patent with the notification number CN1971663A discloses a "dry method for making animal specimens", which uses different concentrations of glycerin to process fixed and dehydrated animal materials step by step, and then immerses them in turpentine oil. Specimens made by making, air-drying and painting. This method of treating with different concentrations of glycerin and immersing in turpentine oil has defects. Because the muscular layer of the gastrointestinal wall of animals is thin and the length of the gastroint...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 付玉洁郑从森陈可彤陈玉莹汤雅婷熊东炜
Owner SHENYANG INST OF TECH
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