A kind of calcium tablet containing ergosterol and preparation method thereof

A technology of ergosterol and calcium tablets, applied in the direction of microorganism-based methods, biochemical equipment and methods, and functions of food ingredients, can solve problems such as poor taste, high price, calcium supplementation effect and product absorption rate to be verified, etc. Achieve the effects of improving permeability, promoting calcium absorption, and improving human immunity

Active Publication Date: 2020-12-18
广东省科学院生物与医学工程研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the research data of the Ministry of Health, 40% of children and 60% of middle-aged and elderly people in my country are deficient in calcium, but most of the existing calcium supplement products on the market are expensive and taste poor, and the calcium supplement effect and product absorption rate have yet to be verified.

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  • A kind of calcium tablet containing ergosterol and preparation method thereof
  • A kind of calcium tablet containing ergosterol and preparation method thereof
  • A kind of calcium tablet containing ergosterol and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The content of ergosterol in the original strain

[0043] (1) Strain purchase

[0044] Saccharomyces cerevisiae 08-h strain was purchased from Guangdong Microbial Culture Collection Center, number GIM 2.182. ,

[0045] (2) Bacteria culture

[0046] Preserved strains at 4°C in slant medium (composition: 1.5% (w / v) yeast extract, 0.5% (w / v) peptone, 1% (w / v) glucose, 2% (w / v) After activation on the agar), inoculate in 8mL liquid seed culture medium (being YPD medium, composition is: 1% (w / v) yeast extract, 2% (w / v) peptone, 2% (w / v) ) glucose) in a test tube, cultured at 120rpm at 28°C for 12h to obtain a first-grade liquid strain. Take 1 mL of the first-grade liquid strain and inoculate it into a 250-mL Erlenmeyer flask containing 50 mL of seed medium, and incubate at 28°C for 20 h at a constant temperature of 150 rpm to obtain the second-grade liquid strain.

[0047] (3) Fermentation broth treatment and ergosterol extraction

[0048] Centrifuge and wash 20mL of b...

Embodiment 2

[0071] Example 2 High pulse electrophoresis (HPEF) treats the yeast to increase the release of ergosterol in the fermentation broth.

[0072] (1) Yeast pretreatment: wash and centrifuge at 2~4℃ (3000r / min, 15min), 0.5% mass volume ratio NaHCO 3 Wash three times, centrifuge (3000r / min, 15min), collect the supernatant, dilute the precipitate 10 times with physiological saline, and set aside.

[0073] (2) Select HPEF parameters: under the pulse width of 13 μs, cycle treatment under the electric field strength of 10, 20, 30, 40 kV / cm respectively, and record the treatment time or the number of cycles. According to the liquid flow rate, pulse frequency, and number of processing cycles in the system, calculate the number of pulses processed by the processing medium, sample at 0, 15, 30, 45, and 60 pulses, and measure the dissolution of protein, amino acids, and ergosterol at the end of the processing , and the yeast suspension not treated with electric field was used as control.

...

Embodiment 3

[0075] Example 3 Preparation of Ergosterol Calcium Tablets

[0076] (1) Cultivate yeast under optimized fermentation conditions (using the optimized medium and culture conditions of Example 1 step (9)), the yeast cells are centrifuged, and the supernatant is collected. °C) to obtain solid material I. The cells were collected and washed twice with sterile water.

[0077] (2) Mix physiological saline and bacteria at a volume ratio of 9:1, and suspend the bacteria. Under the optimal conditions (i.e. 13μs pulse width, 40kV / cm electric field strength, 15 pulse electric fields), the yeast cells were treated with pulse electric field, and after the end, the yeast was centrifuged (3000r / min, 15min), the supernatant was collected, and frozen The supernatant was dried in a drier (0.2 mbar, -50°C) and the solid material II was collected. Solid material I and solid material II were combined to give solid material A.

[0078] (3) Ingredients: Weigh ala milk calcium, milk powder, maltod...

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Abstract

The invention discloses a calcium tablet containing ergosterol and a preparation method thereof. The preparation method comprises the following steps: carrying out aerobic fermentation cultivation to saccharomyces cerevisiae by using a optimized culture medium, and improving the expression quantity of the saccharomyces cerevisiae to the ergosterol; and executing the solid-liquid separation to yeast fermentation liquor, freezing and drying the obtained supernate, to obtain a substance I; after diluting the obtained thallus by using normal saline, executing the solid-liquid separation through the pulsed electric field action, freezing and drying the obtained supernate, to obtain a substance II; mixing the substance I and the substance II, to obtain a substance A; uniformly mixing the substance A, Allah milk calcium, whole milk powder, maltodextrin and a sugarcane extractive, to obtain a mixture; and dissolving the mixture by using the water, spray-drying, pelletizing, drying, size-stabilizing, and tabletting, to obtain the calcium tablet containing the ergosterol. The provided calcium tablet containing the ergosterol is not only good for the absorption of the calcium, but also capable of effectively strengthening the human body immunity.

Description

technical field [0001] The invention belongs to the technical field of health food, in particular to an ergosterol-containing calcium tablet and a preparation method thereof. Background technique [0002] Ergosterol, also known as ergosterol, is the precursor of fat-soluble vitamin D2, which can be converted into vitamin D2 when exposed to ultraviolet light. Ergosterol can partially replace the role of vitamin D2 and promote calcium absorption. Ergosterol can enhance the body's ability to resist diseases and has obvious antibacterial and anti-tumor effects. Ergosterol can also be used as an important pharmaceutical and chemical raw material for the production of sterol drugs such as "cortisone" and "hormone progesterone". [0003] Calcium has an important nutritional effect on people of all age groups and is one of the most important minerals in the human body. According to research data from the Ministry of Health, 40% of children and 60% of middle-aged and elderly people...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/145A23L33/10A23L33/105A23L33/16C12N13/00C12P33/20C12R1/865
CPCA23V2002/00C12N13/00C12P33/20A23V2250/21A23V2250/218A23V2200/324A23V2200/308A23V2250/1578
Inventor 梁磊班雯婷陈瑞荣王庆福黄清铧陈盈盈李奇伟安玉兴
Owner 广东省科学院生物与医学工程研究所
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