Method of subjecting Haematococcus pluvialis to attachment culture with rotary biomembrane reactor to produce astaxanthin

A technology of Haematococcus pluvialis and membrane reactor, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of simple biomass harvesting, small footprint, and increased algae biomass

Active Publication Date: 2017-08-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rotating biofilm reactors have not been used to grow Haematococcus pluvialis for astaxanthin production

Method used

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  • Method of subjecting Haematococcus pluvialis to attachment culture with rotary biomembrane reactor to produce astaxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Effect of Nitrogen Deficiency Stress on Astaxanthin Accumulation at Different Times in Batch Culture Mode in Example 1

[0022] 1) Preparation of seed liquid: Inoculate Haematococcus pluvialis species into BG11 culture solution (sodium nitrate 1500mg / L, dipotassium hydrogen phosphate 40mg / L, magnesium sulfate heptahydrate 75mg / L, calcium chloride dihydrate 36mg / L L, citric acid 6mg / L, ferric ammonium citrate 6mg / L, disodium edetate 1mg / L, sodium carbonate 20mg / L, boric acid 2.86mg / L, manganese chloride tetrahydrate 1.86mg / L, seven Zinc sulfate water 0.22mg / L, sodium molybdate dihydrate 0.39mg / L, copper sulfate pentahydrate 0.08mg / L, cobalt nitrate hexahydrate 0.05mg / L) in the triangular flask, under light intensity 1000-4000lx, room temperature ( 20-30°C, the same below), cultivate for 5-7 days, and obtain a higher density algae liquid as the seed liquid;

[0023] 2) Adhesive culture: take the seed liquid in step 1), transfer it to a rotating biofilm reactor containing...

Embodiment 2

[0028] The impact of different light intensity stress on the accumulation of astaxanthin under the batch culture mode of embodiment 2

[0029] 1) Preparation of seed liquid: Inoculate Haematococcus pluvialis species into BG11 culture solution (sodium nitrate 1500mg / L, dipotassium hydrogen phosphate 40mg / L, magnesium sulfate heptahydrate 75mg / L, calcium chloride dihydrate 36mg / L L, citric acid 6mg / L, ferric ammonium citrate 6mg / L, disodium edetate 1mg / L, sodium carbonate 20mg / L, boric acid 2.86mg / L, manganese chloride tetrahydrate 1.86mg / L, seven Zinc sulfate water 0.22mg / L, sodium molybdate dihydrate 0.39mg / L, copper sulfate pentahydrate 0.08mg / L, cobalt nitrate hexahydrate 0.05mg / L) in a conical flask, under light intensity 1000-4000lx, room temperature , cultured for 5-7d, to obtain higher density algae liquid as seed liquid;

[0030] 2) Adhesive culture: take the seed liquid in step 1), transfer it to a rotating biofilm reactor containing BG11 culture liquid, and carry out...

Embodiment 3

[0035] Example 3 Simultaneously performing nitrogen deficiency stress and high light intensity stress on Haematococcus pluvialis in batch culture mode to induce astaxanthin accumulation

[0036] 1) Preparation of seed liquid: Inoculate Haematococcus pluvialis species into BG11 culture solution (sodium nitrate 1500mg / L, dipotassium hydrogen phosphate 40mg / L, magnesium sulfate heptahydrate 75mg / L, calcium chloride dihydrate 36mg / L L, citric acid 6mg / L, ferric ammonium citrate 6mg / L, disodium edetate 1mg / L, sodium carbonate 20mg / L, boric acid 2.86mg / L, manganese chloride tetrahydrate 1.86mg / L, seven Zinc sulfate water 0.22mg / L, sodium molybdate dihydrate 0.39mg / L, copper sulfate pentahydrate 0.08mg / L, cobalt nitrate hexahydrate 0.05mg / L) in a conical flask, under light intensity 1000-4000lx, room temperature , cultured for 5-7d, to obtain higher density algae liquid as seed liquid;

[0037] 2) Adhesive culture: take the seed liquid in step 1), transfer it to a rotating biofilm r...

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Abstract

The invention discloses a method of subjecting Haematococcus pluvialis to attachment culture with a rotary biomembrane reactor to produce astaxanthin; the method comprises the steps of 1, preparing a seed liquid; 2, performing attachment culture; 3, accumulating biomass, to be specific, upon reaching a stable state, using BG11 culture liquid containing 2.1 g/L of sodium nitrate to replace the culture liquid in the rotary biomembrane reactor, and performing batch or supplemented-batch culture; 4, inducing astaxanthin, to be specific, inducing Haematococcus pluvialis to accumulate astaxanthin by means of nitrogen deficiency stress or/and high light density stress, namely replacing the culture liquid with BG11 culture liquid with no sodium nitrate or/and increasing light intensity to 4500-10500 lx; 5, extracting astaxanthin. The advantage that Haematococcus pluvialis in the rotary biomembrane reactor grows fast in attachment manner is given to full play, biomass with high concentration can be acquired, astaxanthin production efficiency is improve, and harvesting cost is lowered.

Description

technical field [0001] The invention belongs to the application field of microalgae, and in particular relates to a method for producing astaxanthin by culturing Haematococcus pluvialis on the wall using a rotating biofilm reactor. Background technique [0002] Astaxanthin is a carotenoid with a strong antioxidant effect. Its ability to quench singlet oxygen and capture free radicals is more than 10 times higher than that of β-carotene and more than 100 times stronger than vitamin E (see references for details 1: Capelli B, Bagchi D, Cysewski G. Synthetic astaxanthin is significantly inferior to algal-basedastaxanthin as an antioxidant and may not be suitable as a human nutraceutical supplement. Nutrafoods, 2013, 12: 145-152), and can enhance immune response and improve anticancer active. Astaxanthin is often used as a food additive and is widely used in the food and health product industries. Astaxanthin can be synthesized by algae. At present, Haematococcus pluvialis is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/12C12R1/89
CPCC12N1/12C12P23/00
Inventor 魏雪团叶成荫温志友冀志霞陈守文
Owner HUAZHONG AGRI UNIV
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