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Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge

A technology of hydroxyethyl cellulose and soybean protein, which is applied in the field of biomedical materials, can solve the problems such as no composite sponge material of hydroxyethyl cellulose and soybean protein isolate, no three-dimensional scaffold material without shape memory function, etc. Achieving the effects of good biocompatibility, low cost and simple production process

Active Publication Date: 2017-09-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the composite membrane material is suitable for cell adhesion and proliferation, it cannot be used as a three-dimensional scaffold material in the field of tissue engineering because the material itself is relatively dense and does not have good shape memory function.
And there is no report on the preparation of hydroxyethyl cellulose and soybean protein isolate composite sponge material

Method used

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  • Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge
  • Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge
  • Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Hydroxyethylcellulose was dissolved in deionized water at a concentration of 2%. Soy protein isolate was dissolved in 5% NaOH solution with a concentration of 10%. The two solutions are mixed evenly, and the dry weight ratio of soybean protein isolate to hydroxyethyl cellulose / soy protein isolate is between 10% and 70%, and cross-linked with ethylene glycol diglycidyl ether (EDGE). EDGE accounts for 50% of the dry weight of hydroxyethyl cellulose / soy protein isolate, stir and mix at room temperature for 30 minutes, and centrifuge to remove air bubbles. The solution was poured into a mold, firstly frozen at -20°C for 12 hours, then frozen at -80°C for 24 hours, and then placed in a freeze-vacuum drying oven to dry and shape. Cut the composite sponge into 1×1cm sheet, freeze it in liquid nitrogen for 10 minutes, then observe the surface structure of the composite sponge with a scanning electron microscope, then cut the composite sponge into a size of 1×2cm, and wrap it w...

Embodiment 2

[0028] The material prepared in Example 1 is cut into sheets of 1 * 1 * 0.2cm size, and each concentration (the dry weight ratio of hydroxyethyl cellulose / soy protein isolate that soybean protein isolate accounts for is 10%, 30%, 50%, 70%) materials, cut 3 pieces, washed three times with normal saline, then added 10mL of normal saline, kept at 37°C for 30min, and set aside; 4mL of blood was collected from the heart of a New Zealand rabbit, and mixed with normal saline Mix well at a ratio of 1:1.25, then add 200uL of diluted blood to each material soaked in normal saline, and conduct positive and negative control tests at the same time, with deionized water as the positive control and normal saline as the negative control. After 30 minutes, it was centrifuged at 1500 rpm for 10 minutes, and the absorbance value was measured at 545 nm of ultraviolet light by an ultraviolet spectrophotometer to calculate the hemolysis rate.

[0029] The following table 1 is the hemolysis test res...

Embodiment 3

[0034] The sponge material prepared in Example 1 was cut into thin pieces of 1 cm×1 cm×0.3 cm, wrapped in tin foil and marked, and autoclaved at 121° C. for 21 minutes. Remove the autoclaved samples and place them separately in 24-well cell culture plates. 1 mL of cell suspension containing 10% serum at a certain concentration was dropped on the surface of the composite material, and cells at the same concentration and volume were added to blank wells as a negative control group. Put in 37°C CO 2 The cells were cultured in the incubator for 72 hours, and the liquid was replaced at 48 hours. After 72 hours, the 24-well plate was taken out, the culture medium was sucked out, rinsed with PBS for 3 times, and then fixed with an appropriate amount of 4wt% formaldehyde solution at 4°C overnight, then the liquid was sucked out and washed with PBS for 3 times, and the prepared 10ug was added / ml of DiO cell membrane fluorescent staining solution, stained in a dark room for 15-20min,...

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Abstract

The invention discloses a hydroxyethyl cellulose / soybean protein composite sponge with a water sensitive shape memory function and a preparing method of the composite sponge. The preparing method comprises the steps of firstly dissolving hydroxyethyl cellulose and soybean protein into deionized water and a weak NaOH solution respectively, and then preparing the composite sponge through freezing and a vacuum drying method. By changing the proportion of hydroxyethyl cellulose and soybean protein or conducting cross-linking modification on epichlorohydrin or ethylene glycol diglycidyl ether, composite sponge materials with different microstructures and properties can be obtained. The hydroxyethyl cellulose / soybean protein composite sponge has water sensitive type memory function, and the shape and structure before compression can be restored. A cell culture experiments in vitro and a blood compatibility experiment show that the composite material has good compatibility to cell, blood and tissue. The method is simple and practical, raw materials are wide in source, and a natural macromolecule biological medical material with an application prospect can be expected to be shaped.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a hydroxyethyl cellulose / soybean protein composite sponge with a water-sensitive shape memory function and a preparation method thereof. Background technique [0002] As the most abundant natural polymer in nature, cellulose has been widely used in industries such as textile, paper making, agriculture and food. In recent decades, cellulose has also been used as a functional biomaterial and tissue engineering scaffold material due to its safety and biocompatibility properties. Due to the development and utilization of cellulose, cellulose derivatives such as cellulose acetate, hydroxyethyl cellulose (HEC), hydroxypropyl cellulose (HPC), carboxymethyl cellulose (CMC), methyl cellulose, cyanide Ethyl cellulose has also begun to be recognized and utilized by everyone. Among them, HEC and CMC are easily soluble in water, can be degraded in vivo or dissolved in body fl...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L1/28C08K5/1515C08J9/28
CPCC08J9/28C08J2301/28C08J2389/00C08L1/284C08L89/00C08L2201/06C08L2201/12C08L2203/02C08K5/1515
Inventor 陈云赵言腾易丽赵亚楠
Owner WUHAN UNIV
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