Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge

A technology of hydroxyethyl cellulose and soybean protein, which is applied in the field of biomedical materials, can solve the problems such as no composite sponge material of hydroxyethyl cellulose and soybean protein isolate, no three-dimensional scaffold material without shape memory function, etc. Achieving the effects of good biocompatibility, low cost and simple production process

Active Publication Date: 2017-09-08
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the composite membrane material is suitable for cell adhesion and proliferation, it cannot be used as a three-dimensional scaffold material in the field of tissue engineering because the mat

Method used

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  • Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge
  • Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge
  • Hydroxyethyl cellulose/soybean protein composite sponge with water sensitive shape memory function and preparing method of composite sponge

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] [Example 1]

[0025] The hydroxyethyl cellulose was dissolved in deionized water at a concentration of 2%. The soy protein isolate was dissolved in 5% NaOH solution with a concentration of 10%. Mix the two solutions uniformly, the dry weight ratio of soy protein isolate in hydroxyethyl cellulose / soy protein isolate is between 10% and 70%, and cross-linked with ethylene glycol diglycidyl ether (EDGE), EDGE accounts for 50% of the dry weight of both hydroxyethyl cellulose and soy protein isolate. Stir and mix for 30 minutes at room temperature, and centrifuge to remove air bubbles. Pour the solution into the mold, first freeze at -20°C for 12h, freeze at -80°C for 24h, and then put it into a freeze vacuum drying oven to dry and shape. Cut the made composite sponge into a sheet of 1×1cm, put it in liquid nitrogen and freeze for 10 minutes, then observe the surface structure of the composite sponge with a scanning electron microscope, then cut the composite sponge into a siz...

Example Embodiment

[0027] [Example 2]

[0028] Cut the material prepared in Example 1 into pieces of 1×1×0.2cm size, each concentration (the dry weight ratio of soy protein isolate in hydroxyethyl cellulose / soy protein isolate is 10%, Cut 3 pieces of materials containing 30%, 50%, 70%), wash them three times with normal saline, and then add 10 mL of normal saline, incubate at 37°C for 30 min, and use them for later use; take 4 mL of blood from the heart of a New Zealand rabbit and press it with normal saline. Mix well at a ratio of 1:1.25, then add 200uL of diluted blood to each material soaked in saline, and perform positive and negative control tests at the same time, and use deionized water as the positive control and physiological saline as the negative control, and incubate them. After 30 min, centrifuge at 1500 rpm for 10 min, measure the absorbance value at 545 nm of ultraviolet light by an ultraviolet spectrophotometer, and calculate the hemolysis rate.

[0029] Table 1 below shows the hemol...

Example Embodiment

[0033] [Example 3]

[0034] The sponge material prepared in Example 1 was cut into thin pieces of 1cm×1cm×0.3cm, wrapped in tin foil and marked, and autoclaved at 121°C for 21min. Take out the autoclaved samples and put them into 24-well cell culture plates. Drop 1 mL of a certain concentration of 10% serum cell suspension on the surface of the composite material, and add cells of the same concentration and the same volume in the blank hole as a negative control group. Put in 37℃CO 2 Cultivate in the cell culture box for 72 hours, and change the liquid at 48 hours. After 72h, take out the 24-well plate, aspirate the medium, rinse with PBS 3 times, fix with an appropriate amount of 4wt% formaldehyde solution at 4℃ overnight, then aspirate the liquid and wash 3 times with PBS, add the prepared 10ug / ml DiO cell membrane fluorescent staining solution, stained in a dark room for 15-20min, then washed 3 times with PBS, and observed with a fluorescence microscope.

[0035] image 3 It...

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Abstract

The invention discloses a hydroxyethyl cellulose/soybean protein composite sponge with a water sensitive shape memory function and a preparing method of the composite sponge. The preparing method comprises the steps of firstly dissolving hydroxyethyl cellulose and soybean protein into deionized water and a weak NaOH solution respectively, and then preparing the composite sponge through freezing and a vacuum drying method. By changing the proportion of hydroxyethyl cellulose and soybean protein or conducting cross-linking modification on epichlorohydrin or ethylene glycol diglycidyl ether, composite sponge materials with different microstructures and properties can be obtained. The hydroxyethyl cellulose/soybean protein composite sponge has water sensitive type memory function, and the shape and structure before compression can be restored. A cell culture experiments in vitro and a blood compatibility experiment show that the composite material has good compatibility to cell, blood and tissue. The method is simple and practical, raw materials are wide in source, and a natural macromolecule biological medical material with an application prospect can be expected to be shaped.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a hydroxyethyl cellulose / soybean protein composite sponge with a water-sensitive shape memory function and a preparation method thereof. Background technique [0002] As the most abundant natural polymer in nature, cellulose has been widely used in industries such as textile, paper making, agriculture and food. In recent decades, cellulose has also been used as a functional biomaterial and tissue engineering scaffold material due to its safety and biocompatibility properties. Due to the development and utilization of cellulose, cellulose derivatives such as cellulose acetate, hydroxyethyl cellulose (HEC), hydroxypropyl cellulose (HPC), carboxymethyl cellulose (CMC), methyl cellulose, cyanide Ethyl cellulose has also begun to be recognized and utilized by everyone. Among them, HEC and CMC are easily soluble in water, can be degraded in vivo or dissolved in body fl...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L1/28C08K5/1515C08J9/28
CPCC08J9/28C08J2301/28C08J2389/00C08L1/284C08L89/00C08L2201/06C08L2201/12C08L2203/02C08K5/1515
Inventor 陈云赵言腾易丽赵亚楠
Owner WUHAN UNIV
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