Scenedesmus sp. as well as culture method and application thereof

A technology of Scenedesmus and microalgae oil, which is applied in the fields of biotechnology and bioenergy, can solve the problems of the decline of microalgae's ability to produce oil, and achieve the effects of high added value, fast growth rate, and high total lipid content

Active Publication Date: 2017-09-19
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem in the prior art that the ability of microalgae to produce oil decreases significantly when the culture temperature

Method used

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  • Scenedesmus sp. as well as culture method and application thereof
  • Scenedesmus sp. as well as culture method and application thereof
  • Scenedesmus sp. as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Scenedesmus HCS-02 obtained by isolation and domestication screening

[0021] (1) Obtaining the starting algae strain: In June 2011, 150mL of water samples were retrieved from the upper reaches of the Hunchun River in Jilin, filtered with gauze to remove large impurities, and 50ml of the filtered water samples were inoculated into 200mL of BG11 medium The enrichment culture was carried out in the medium, the light intensity of the culture was 5000Lux, the temperature was 25°C, the light-dark cycle was 24h, and the light-dark time ratio was 14:10. After about half a month of culture, the culture medium turned green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cu...

Embodiment 2

[0026] Identification of strains

[0027] The DNA of HCS-02 algal cells was extracted by CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Scenedesmus sp. There is the greatest similarity, its BLASTn value is 2610, the Max index value is 99%, it can be determined that HCS-02 is Scenedesmus ( Scenedesmus sp. ).

Embodiment 3

[0029] Application of Scenedesmus HCS-02

[0030] The algae liquid of HCS-02 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 2 and Table 3. The cultivation was carried out in a 500mL shake flask, and the OD of the culture solution after inoculation was 689.5 is 0.1. During the culture process, the light intensity was 5000 Lux, the culture temperature was 25°C, the light cycle was 24 hours, the light-dark time ratio was 14:10, the shake flask was shaken every 8 hours to make the sediment float up, and the culture time was 20 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, and the starch content of the algae cells was determined by the amylase hydrolysis method after breaking the cell wall with enzyme / ultrasound. The biomass parameter of HCS-02 cultured for 20 days was measured to be 2.0337g / L, and the starch content of algal cells accounted for ...

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Abstract

The invention discloses a strain of scenedesmus sp. as well as a culture method and application thereof. The strain is HCS-02, is classified and named as scenedesmus sp., is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2015, and has a preservation number of CGMCC No. 10766. The scenedesmus sp. can tolerate lower culture temperature, is large in culture temperature interval and can be easily cultured in an open type. The scenedesmus sp. shows a rapider biomass accumulation speed under different culture conditions; the obtained biomass is rich in starch and oil, thus being used as feed or used for producing biodiesel after being treated.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a scenedesmus capable of producing microalgae oil through low-temperature cultivation and its cultivation application. Background technique [0002] In recent years, with the rapid development of the world economy, the advancement of industrialization, and the continuous growth of population, the world's demand for petroleum fuels is increasing day by day. At the same time, due to the extensive use of petroleum-based fuels, a large amount of industrial waste gas and greenhouse gases are emitted into the atmosphere, causing a series of environmental problems, such as the greenhouse effect and smog. Therefore, the research on high-efficiency renewable energy has become a current research hotspot for petroleum alternative fuels. Microalgae has a simple structure, and the whole organism can carry out photosynthesis, which has the characteristics of high photos...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463C12N1/125C12R2001/89Y02E50/10
Inventor 王鹏翔师文静廖莎孙启梅王领民高大成
Owner CHINA PETROLEUM & CHEM CORP
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