Method for efficiently preparing olive oil from olive fruit
A technology of olive fruit and olive oil, which is applied in the field of producing olive oil by enzymatic method and its waste reuse, can solve the problem of high cost of wastewater treatment, and achieve the benefits of recycling, weakening emulsification, and reducing the risk of environmental pollution Effect
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Embodiment 1
[0043] Weigh 100kg of olive fruit, grind it twice on a common grinder, add it to 200L of warm water at 35°C, and stir well to obtain a feed solution; use 0.5mol / L hydrochloric acid aqueous solution to adjust the pH of the feed solution to 6, and add amylase to the final concentration 120U / L, cellulase to a final concentration of 100U / L, enzymatic hydrolysis for 4h. Add temperature-sensitive acid protease (rapidly inactivated above 45°C) to the enzymolysis material again to a final concentration of 80 U / L, enzymatically hydrolyze for 3 hours, and keep warm at 55°C for 35 minutes to obtain material solution I. The feed liquid I is centrifuged on a decanter centrifuge to obtain liquid and wet residue, and the liquid directly enters the disc separator to separate olive oil and waste water I. The obtained olive oil is dried under vacuum condition, left standing and then filtered to obtain the finished olive oil, and the finished oil is inspected. Wastewater I is adsorbed by 732 ca...
Embodiment 2
[0045] Weigh 200kg of olive fruit, grind 3 times on a common grinder, add 1000L effluent liquid I, heat to about 40°C, and stir well to obtain feed liquid II; use 0.5moL / L hydrochloric acid aqueous solution to adjust the pH of the feed liquid to 5.5, add starch Enzyme to the final concentration of 100U / L, cellulase to the final concentration of 80U / L, enzymatic hydrolysis for 3h. Add temperature-sensitive acid protease (rapidly inactivated above 45°C) to the enzymatic hydrolysis solution I again to a final concentration of 80 U / L, enzymatic hydrolysis for 3 hours, and keep warm at above 55°C for 35 minutes to obtain feed solution III. Centrifuge the material liquid III on a decanter centrifuge to obtain liquid and wet residue, and the liquid directly enters the disc separator to separate olive oil and waste water II. The obtained olive oil is dried under vacuum condition, left standing and then filtered to obtain the finished olive oil, and the finished oil is inspected. The ...
Embodiment 3
[0047] 100kg of waste water and 500kg of wet slag from circulating oil production are used as raw materials. After mixing, sterilize at 121°C for 25 minutes, add 90kg of Bacillus subtilis powder (15%) after cooling, cultivate for 48 hours in aerobic fermentation at 37°C, and refrigerate at 0-5°C Get leavening agent.
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