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Composite bacteria agent for decolorization and flocculation and preparation method of flocculant thereof

A technology of compound bacterial agent and flocculant, applied in the field of water purification, can solve the problems of metal corrosion, strong carcinogenicity, and color.

Active Publication Date: 2020-08-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aluminum ions in aluminum-based flocculants can easily cause Alzheimer's disease; iron ions in iron-based flocculants have a corrosive effect on metals, which can easily cause color in the treated water and increase the load of sludge treatment; PAM monomers have strong neurotoxicity. Toxic, highly carcinogenic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Pseudoalteromonas Pseudoalteromonas sp. GHS18 and Pseudomonas alternata Pseudoalteromonas A flocculant made from the exopolysaccharide obtained by co-fermentation of sp. JP. Among them, Pseudoalteromonas Pseudoalteromonas The taxonomic designation of sp. GHS18 is Pseudoalteromonas ( Pseudoalteromonas sp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms, with the preservation number CGMCC No. 12913. Pseudomonas alternata Pseudoalteromonas The taxonomic designation of sp. JP is Pseudomonas alternata ( Pseudoalteromonas sp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No. 12914. The General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms is located at No. 3, Yard No. 1, Beichen West Road, Chaoyang District...

Embodiment 2

[0027] The preparation method of flocculant comprises the following steps:

[0028] 1) Seed liquid culture: Inoculate the compound bacterial agent on the slant medium, and after cultivating at 23°C for 72 hours, add sterile water 1.3 times the volume of the medium, and shake to obtain the seed liquid; among them, the pseudo-alternating single Bacteria Pseudoalteromonas sp. GHS18 and Pseudomonas alternata Pseudoalteromonas The mass ratio of sp. JP is 1:2. The composition and concentration of the slant medium are glucose 18g / L, urea 0.51g / L, ammonium sulfate 0.24g / L, yeast extract 0.4g / L, dipotassium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 1.7g / L , agar 15g / L and the remainder aged seawater or artificial seawater; adjust the pH to 7.4, and autoclave at 121°C for 30min. Among them, the artificial seawater formula is: sodium chloride 25.0 g / L, magnesium chloride 1.9 g / L, magnesium sulfate 3.1 g / L, calcium chloride 1.3 g / L, sodium bicarbonate 0.2 g / L, potassium...

Embodiment 3

[0032] Example 3: Determination of flocculation activity

[0033]Adopt the flocculation activity test method (R.Kurane, K.Takeda, T.Suzuki.Screening and Characteristics of Microbial Flocculants.Agric.Biol.Chem.1986:2301-2307) to the kaolin suspension turbid liquid 93ml, add 5ml 1% (m / v) CaCl 2 As a coagulation aid, add 2ml of the sample of Example 2, adjust the pH value to 9, mix and stir quickly for 1min, then stir slowly for 5min, let stand for 10min, take the mixed solution at 1cm below the water level, and measure the absorbance A at 550nm with a visible spectrophotometer , while using 2ml of distilled water instead of the sample as a control, measure the absorbance B, and calculate the flocculation rate, FR(%) = (A-B) / A×100%. The flocculation rate of marine bacterial decolorization flocculant to kaolin suspension is 93.72%.

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PUM

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Abstract

The invention discloses a composite bacterial agent for decolorization and flocculation and a preparation method thereof. The composite bacterial agent includes pseudoalteromonas with a preservation number of CGMCC No.12913 Pseudoalteromonas sp.GHS18 and Pseudomonas alternata with accession number CGMCC No.12914 Pseudoalteromonas sp. JP. The flocculant was prepared from the exopolysaccharide obtained by the synergistic fermentation of the compound bacterial agent. The beneficial effects are: the prepared decolorizing flocculant has a good decolorizing effect, and the flocculation rate of kaolin reaches more than 92%; it has high activity for decolorizing high-concentration wastewater, and the decolorizing rate of methylene blue, malachite green, and crystal violet reaches 95%. Above, above 99.6% and above 99%, have no direct or potential toxicity to organisms, can be naturally degraded, green and healthy, and pollution-free.

Description

technical field [0001] The invention relates to the technical field of water purification, in particular to a preparation method of a composite bacterial agent for decolorization and flocculation and a flocculant thereof. Background technique [0002] Flocculation technology is widely used in water supply, wastewater flocculation purification, fermentation industry, solid-liquid separation, etc. The flocculation effect depends on the type of flocculant selected. At present, there are two main types of flocculants used in the water treatment industry: inorganic flocculants represented by aluminum-based, iron-based and their polymers, and organic polymer flocculants represented by PAM and their derivatives. They are widely used due to their good flocculation effect and low cost during use, but their unsafety to the environment has attracted enough attention. Aluminum ions in aluminum-based flocculants can easily cause Alzheimer's disease; iron ions in iron-based flocculants h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/01
CPCC02F1/56C02F2303/00C12N1/20C12P19/04C12N1/205C12R2001/01
Inventor 穆军崔霞王玉霞
Owner ZHEJIANG OCEAN UNIV