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Construction method and application of high performance liquid chromatography characteristic map for online extraction of fresh Cordyceps sinensis

A high-performance liquid chromatography and Cordyceps sinensis technology, applied in the application field of online extraction and analysis, can solve the problems of not being able to realize online analysis in a real sense, connecting into an online analysis system, and difficulties in actual production and application, so as to achieve simple operation and promote Simple application and installation

Active Publication Date: 2020-12-18
DONGGUAN HEC CORDYCEPS R&D CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are related studies on the pretreatment system of online liquid chromatography medium, but there are certain limitations, such as the device provided by the patent "Liquid Phase Medium Pretreatment Device for Online Chromatographic Analysis" (Patent No. ZL 200720074371.X) In addition to the pretreatment of the liquid medium, it can also achieve the purpose of large flow, anti-blocking and basic elimination of air bubbles, but this device does not really connect it with the liquid chromatograph into an online analysis system, and it cannot be realized in a real sense. On-line analysis; In addition, there are liquid chromatography on-line desalination, enrichment and mass spectrometry on-line system (patent number ZL 200610134981.4) and solid phase extraction-liquid chromatography on-line analysis of trace organics in water (patent number ZL 200610023536.0) related reports
However, these studies have limitations and incompleteness
In recent years, literature reports have shown that online sample extraction-HPLC analysis technology has the advantages of fast speed, sample saving and high degree of automation compared with traditional offline extraction technology, and has been successfully applied to the component analysis of Chinese herbal medicines. However, the reported literature The method generally needs to add six-way valves, pressurization devices, enrichment columns and other devices to the conventional liquid phase system, which makes it difficult to apply in actual production
For example, the patent "A High Performance Liquid Chromatography Online Analysis Method and Its Application" (Patent No. ZL 201310107475.6) provides a high performance liquid chromatography online analysis method. The system includes a sample pretreatment system, a high performance liquid chromatography, an editable logic control Although the online detection of sample content and component ratio has been realized with devices such as detectors and touch screens, there are many devices in the system and the operation is cumbersome.

Method used

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  • Construction method and application of high performance liquid chromatography characteristic map for online extraction of fresh Cordyceps sinensis
  • Construction method and application of high performance liquid chromatography characteristic map for online extraction of fresh Cordyceps sinensis
  • Construction method and application of high performance liquid chromatography characteristic map for online extraction of fresh Cordyceps sinensis

Examples

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Effect test

Embodiment 1

[0043]Example 1: Online extraction of fresh Cordyceps sinensis and establishment of high performance liquid phase characteristic map

[0044]1.1 Instruments and reagents

[0045]Instrument: High performance liquid chromatograph: Agilent 1260, including online degasser, quaternary pump, autosampler, column thermostat, DAD detector; Quadrupole mass spectrometer: Agilent 6130; 1 / 100,000 Analytical balance: Mettler ToledoXS204; one-millionth analytical balance: Mettler Toledo XP26; online extractor: Phenomenex, Security Guard; guard column: Agilent Zorbax SB-AQ, specification of 4.6mm×12.5, 5μm; Column: Agilent Zorbax SB-AQ, the specification is 4.6mm×150, 5μm.

[0046]Reagents: methanol: Spectrum, chromatographically pure; Acetic acid: Aladdin, chromatographically pure; ultrapure water: prepared by Milli-Q ultrapure water system; reference substance: uracil, guanine, adenine, uridine, inosine Adenosine was purchased from Wuhan Yuancheng Biotechnology Co., Ltd.; Guanosine was purchased from Beij...

Embodiment 2

[0059]Example 2: On-line extraction of chemical components in dried Cordyceps sinensis and Cordyceps militaris by high performance liquid chromatography tandem mass spectrometry

[0060](1) Preparation of the test product extractor: the same as in Example 1.

[0061](2) Online extraction: the same as the first embodiment.

[0062](3) High performance liquid chromatography tandem mass spectrometry detection: the same as in Example 1.

[0063](4) Test results: Analyze according to the above chromatographic conditions to obtain the high performance liquid chromatograms of dried Cordyceps and Cordyceps militaris, seeimage 3 withFigure 4 . Confirmed by comparing the high performance liquid chromatograms of the mixed reference substance, dried Cordyceps and Cordyceps militaris, as well as the ion fragments extracted from nucleoside substances reported in the literature, a total of 12 main chromatographic peaks were obtained in the high performance liquid chromatogram of dried Cordyceps Signal, 7 of w...

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Abstract

The invention belongs to the technical field of chromatography and particularly discloses an online extraction and high-performance liquid characteristic spectrum construction method for fresh Cordyceps sinensis, characterized by comprising the steps of (1) preparing an extractor; (2) performing online extraction; (3) detecting by high-performance liquid chromatography tandem mass spectrometry; (4) constructing characteristic spectra. A system apparatus of online extraction and high-performance liquid chromatography is simple to operate, needs low quantity of samples, consumes little time, provides greatly promoted application for the method in actual production, and has a promising application prospect.

Description

Technical field[0001]The invention relates to the technical field of chromatographic analysis, in particular to a method for constructing an online extraction high-performance liquid phase characteristic map of fresh cordyceps and its application in the online extraction and analysis of active ingredients of Chinese medicinal materials.Background technique[0002]Cordyceps sinensis is a dry complex of the larvae and corpses of the larvae of the larvae of the Hepidiaceae fungus Cordyceps sinensis (BerK.) Sacc.). It is sweet in nature and flavor, calming, returning to the lung and kidney meridian. It has the effects of invigorating the kidneys and lungs, stopping bleeding and resolving phlegm. It is used to treat kidney deficiency and essence deficiency, impotence and nocturnal emission, waist and knee soreness, chronic cough and dyspnea, cough and hemoptysis. Modern research shows that Cordyceps contains nucleosides, proteins, polysaccharides, sterols and other active substances, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686G01N30/88
Inventor 钱正明周妙霞邱健健李春红李文佳
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD