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Three-dimensional nanofiber stent based on microfluidic technology and electrospinning technology and preparation method thereof

A three-dimensional nano-fiber scaffold technology, used in electrospinning, fiber processing, pharmaceutical formulations, etc., can solve problems such as low long-term patency rate and lumen narrowing, and achieve improved long-term patency rate and good biocompatibility. , the effect of promoting growth and migration

Inactive Publication Date: 2018-04-03
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of acute thrombosis and lumen stenosis of small-caliber artificial blood vessels in the prior art, as well as the low long-term patency rate

Method used

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  • Three-dimensional nanofiber stent based on microfluidic technology and electrospinning technology and preparation method thereof
  • Three-dimensional nanofiber stent based on microfluidic technology and electrospinning technology and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Remove sericin on the surface of silk fiber:

[0034] Bombyx mori silk was exposed to 0.1% Na 2 CO 3 In the solution, treat at 98-100 DEG C for 30 minutes, repeat three times to remove sericin on the surface of silk fiber, and dry in air at room temperature.

[0035] (2) Preparation of silk fibroin solution:

[0036] Dissolve the degummed silk in 9.3mol / L lithium bromide aqueous solution at the ratio of 27g:100mL, put it in an oven at 60°C for 4 hours, and obtain the silk fibroin solution after dialysis, which is ready for use. And take a part of the silk fibroin solution to freeze-dry to obtain the silk fibroin sponge, which is set aside.

[0037] (3) Preparation of microfluidic chip:

[0038] The SU-8 template of the vertical microchannel was made by soft lithography technology, A glue and B glue were stirred and mixed, poured on the SU-8 template and the blank template, placed on the horizontal test bench for 30 minutes to remove air bubbles, and then placed ...

Embodiment 2

[0046] (1) Using microfluidic technology to prepare silk fibroin microdroplets loaded with growth factors:

[0047] A microfluidic chip device capable of preparing monodisperse silk fibroin microdroplets is adopted, including micropumps, microfluidic chips, droplet receiving and storage containers. The silk fibroin solution prepared in Example 1 was added with growth factors required for embedding and sustained release as a dispersed phase; a mixture of Span 80 and medium-chain fat triglycerides was used as a continuous phase, and the mixing ratio was 2%. Using different flow rates or pressure ratios, the microfluidic parameters are preferably as follows: the dispersed phase is 20mba-120mba, the continuous phase is 150mba-600mba, and sheared into monodisperse droplets encapsulating growth factors. The droplets then pass through the collection tube into a collection sump filled with Span 80 oil phase. (2) Preparation of silk fibroin microspheres:

[0048] Cool the collected d...

Embodiment 3

[0052] (1) Using microfluidic technology to prepare silk fibroin microdroplets loaded with growth factors:

[0053] A microfluidic chip device capable of preparing monodisperse silk fibroin microdroplets is adopted, including micropumps, microfluidic chips, droplet receiving and storage containers. The silk fibroin solution prepared in Example 1 was added with growth factors required for embedding and sustained release as a dispersed phase; a mixture of polyoxyethylene hydrogenated castor oil and sunflower oil was used as a continuous phase, and the mixing ratio was 2%. Using different flow rates or pressure ratios, the microfluidic parameters are preferably: the dispersed phase is 20mba-120mba, the continuous phase is 150mba-600mba, and sheared into monodisperse droplets encapsulating growth factors. The droplets then pass through a collection tube into a collection tank filled with polyoxyethylene hydrogenated castor oil oil phase.

[0054] (2) Preparation of silk fibroin m...

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Abstract

The invention relates to the fields of a microfluidic technology and an electrospinning technology, and provides a method for preparing a three-dimensional nanofiber stent with a micro-nanometer composite structure. By the microfluidic technology, monodisperse silk fibroin microspheres with a uniform dimension are prepared; growth factors are slowly and stably released along with the silk microsphere degradation, so that the biological effect is fully achieved; and the burst release problem and the peak valley phenomenon generated by nonuniform microspheres prepared through mechanical stirring, oscillation, ultrasonic homogenate and the like in the prior art are solved. In addition, double spray heads or a single spray head is used for performing electrostatic spinning on an ethanol solution of the silk fibroin microspheres containing growth factors and PEO (Polyethylene Oxide), and a hexafluoroisopropanol solution of silk fibroin; then, PEO fiber in the solution is dissolved; and themicro-nanometer composite three-dimensional nanofiber stent with the uniform porous structure and the proper porosity is prepared. The stent has good biocompatibility and certain mechanical performance; the inward growth of cells is facilitated; the increase and the migration of the cells are promoted; the double functions of improving the small-caliber artificial blood vessel forward patency rateand remodeling the blood vessel wall are realized; and the stent is applicable to the restoration or the replacement of injury or lesion small-caliber blood vessel tissues.

Description

technical field [0001] The present invention relates to the field of microfluidic technology and electrospinning technology, in particular to a microfluidic technology to prepare uniform monodisperse microspheres and to combine monodisperse microspheres with nanofibers through electrospinning technology to prepare "micro- A method for three-dimensional nanofibrous scaffolds of "nano" composite structures. Background technique [0002] Cardiovascular disease poses a major threat to human health. At the beginning of the 21st century, 17 million people died of cardiovascular and cerebrovascular diseases in the world. By 2020, this number will reach 25 million. With the rising incidence of cardiovascular diseases, vascular reconstruction plays an increasingly important role in clinical surgery. However, the sources of autologous blood vessels are very limited, and there is an urgent need for vascular graft substitutes of various calibers. At present, large-diameter artificial b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/18A61L27/50A61L27/54A61L27/56D04H1/728D01D5/00
CPCA61L27/18A61L27/227A61L27/50A61L27/54A61L27/56A61L2300/414A61L2300/602A61L2400/12D01D5/003D01D5/0061D01D5/0092D04H1/728C08L89/00C08L71/02
Inventor 刘海峰刘强樊瑜波
Owner BEIHANG UNIV
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