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Substrate, preparation method thereof, mass spectrometric analysis detection method for metabolic molecules

A mass spectrometry analysis and detection method technology, applied in the field of material synthesis and mass spectrometry detection, can solve the problems of affecting the ionization efficiency of the sample to be tested, uneven crystallization of organic matrix, poor repeatability of detection results, etc., and achieves good protein resistance and time-consuming. Short, controllable effects

Pending Publication Date: 2018-07-31
浙江亿纳谱生命科技有限公司
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Problems solved by technology

However, MALDI-TOF-MS also has limitations: analysis of small molecules (m / z<1000) by MALDI-TOF-MS is usually limited
This is because traditional organic matrices such as α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) will have a large number of background matrix mass spectrometry signals in the low mass range, seriously Determination of Interfering Small Molecules
At the same time, these organic matrices are not uniformly crystallized, so point-to-point effects will occur during mass spectrometry detection, resulting in poor repeatability of detection results
In addition, for a simple standard sample system, the detection limit of MALDI-TOF-MS can still reach 10-15 ~ 10-18, but in biological samples such as serum, the existence of a large amount of interference substances such as proteins and salts greatly Affected the ionization efficiency of the sample to be tested
Therefore, the traditional matrix is ​​difficult to meet the demand for small molecule detection, and a new type of matrix material that can be used for biological system detection and has certain anti-interference and salt tolerance needs to be developed urgently

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  • Substrate, preparation method thereof, mass spectrometric analysis detection method for metabolic molecules
  • Substrate, preparation method thereof, mass spectrometric analysis detection method for metabolic molecules
  • Substrate, preparation method thereof, mass spectrometric analysis detection method for metabolic molecules

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preparation example Construction

[0042] The embodiment of the present invention also provides a preparation method of a substrate, the preparation method comprising:

[0043] Provide a homogeneous alkaline solution containing zinc ions;

[0044] Hydrothermal reaction of alkaline solution to obtain particulate matter;

[0045] The dried particles are suspended in a liquid dispersion medium.

[0046] Alternatively, the alkaline solution is an aqueous solution in which zinc acetate and sodium hydroxide are dispersed. For example, the aqueous solution is prepared by dissolving zinc acetate in water as a solvent to obtain a zinc acetate solution; and uniformly dispersing sodium hydroxide in the zinc acetate solution.

[0047] The conditions for the hydrothermal reaction were 180° C. and 2 hours.

[0048] Preferably, the particulate matter is obtained by washing the hydrothermal reaction product with a mixture of ethanol and deionized water.

[0049] More preferably, the method of drying the particulate matter ...

Embodiment 1

[0060] Preparation of star-shaped zinc oxide nanoparticle matrices.

[0061] The preparation method includes the following steps:

[0062] Step 1: use water as a solvent to prepare a zinc acetate solution;

[0063] Step 2: add sodium hydroxide to the above solution, and stir magnetically at room temperature until the solution is homogeneously dispersed;

[0064] Step 3: move the mixed solution obtained in the second step to a PTFE-lined stainless steel hydrothermal reactor, and keep the reaction at 180° C. for 2 hours to form star-shaped zinc oxide nanoparticles;

[0065] Step 4: Repeatedly washing the zinc oxide star-shaped nanoparticles obtained in the third step with ethanol and deionized water, and drying at 60° C. into powder.

[0066] Nanoparticles in the matrix are characterized by:

[0067] Instrumentation used for characterization: Size and morphology characterization of the product was done on a Hitachi S-4800 scanning electron microscope (SEM).

[0068] The char...

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Abstract

The invention provides a substrate, a preparation method thereof and a mass spectrometric analysis detection method for metabolic molecules, and belong to the field of material synthesis and mass spectrometry. A zinc oxide substrate is synthesized by a hydrothermal method with sodium hydroxide and zinc acetate as raw materials, conditions are easy to control, the time consumed is short, and the operation is simple. The prepared zinc oxide substrate is star-shaped nanoparticles assembled by sword-shaped nanorods with sizes of less than 2 microns, particle sizes are uniform, and the substrate has good ultraviolet absorption properties, thereby having good properties being helpful of ionization analysis the a sample molecular. Compared with a spherical nano-zinc oxide substrate, the star-shaped zinc oxide substrate has smaller background noise, can remove the background interference and hot spot effect of traditional substrates, meanwhile, has better salt-tolerant and protein-resistant properties, and can be used for complex biological sample systems such as serums and the like. Simultaneously, sample pretreatment operations such as enrichment, separation and the like are not requiredin the detection process, and the efficient and rapid detection of the metabolic molecules is achieved.

Description

technical field [0001] The invention relates to the fields of material synthesis and mass spectrometry detection, in particular to a matrix and a preparation method thereof, and a mass spectrometry analysis and detection method of metabolites. Background technique [0002] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a new type of soft ionization biological mass spectrometry developed in recent years. Perform accurate qualitative and quantitative analysis. At present, the molecular weight of biological macromolecules can be determined up to 600KDa. Due to its large detectable molecular weight range, fast scanning speed, high resolution, high sensitivity, simple instrument structure and other significant advantages, it is widely used in many frontier fields in life sciences, such as proteomics. , drug analysis, etc., have been widely used. However, MALDI-TOF-MS also has limitations: the analysis of small molecules (m / z < ...

Claims

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Application Information

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IPC IPC(8): G01N27/64
CPCG01N27/64Y02A50/30
Inventor 万晶晶
Owner 浙江亿纳谱生命科技有限公司
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