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Bone repair material allowing injection of multi-pore structure and preparation method of bone repair material

A pore structure and bone repair technology, applied in pharmaceutical formulations, medical science, prostheses, etc., can solve the problems of insufficiently combined pore structure without cell growth, etc., to promote the transmission of nutrients and promote cell adhesion and growth. Effect

Active Publication Date: 2018-11-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a bone repair material with injectable multiple pore structure and its preparation method, to solve the problem that the existing bone repair material does not have a pore structure suitable for cell growth and the gap between the existing bone repair material and the autologous bone. The combination is not tight enough and other problems

Method used

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  • Bone repair material allowing injection of multi-pore structure and preparation method of bone repair material
  • Bone repair material allowing injection of multi-pore structure and preparation method of bone repair material
  • Bone repair material allowing injection of multi-pore structure and preparation method of bone repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] In this example, blank polylactic-co-glycolic acid (PLGA) porous microspheres and HA composite PLGA porous microspheres (HA-PLGA porous microspheres) with different hydroxyapatite (HA) contents were prepared.

[0052] 1. Preparation of PLGA porous microspheres

[0053] Weigh 1.8g of PLGA and add it to 36mL of dichloromethane, stir well at room temperature, the molecular weight of PLAG is 8.5w, and the mass ratio of LA and GA is LA / GA=85 / 15, then add 0.025g / mL of bicarbonate Ammonium solution 6mL, ultrasonic emulsification treatment for 5min, add 0.025g / mL ammonium bicarbonate solution 6mL, ultrasonic emulsification treatment for 15min, to obtain a uniformly dispersed W / O emulsion, transfer the W / O emulsion to 300mL10g / L polyvinyl alcohol (PVA) solution to obtain a W / O / W double emulsion, add 300mL deionized water, stir overnight to ensure that the dichloromethane is completely evaporated, wash with deionized water several times, and filter through a filter screen with a ...

Embodiment 2

[0069] In this example, the preparation of dopamine-hyaluronic acid (HA-DOPA), the steps are as follows:

[0070] (1) To the sodium hyaluronate (Mw=340kDa) aqueous solution with a concentration of 11.5mg / mL, add a solution of N-hydroxysuccinimide (NHS) with a concentration of 46mg / mL dropwise, and then add a solution with a concentration of 150mg / mL 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) solution, stirred for 2 hours, added dropwise an aqueous solution of dopamine hydrochloride with a concentration of 2 mmol / L, stirred for 12 hours, two The pH value was controlled at 5.0 during the stirring reaction process, and the operation of this step was carried out under nitrogen protection. The molar ratio of EDCI, NHS, dopamine hydrochloride and the carboxyl group on sodium hyaluronate was 6:4:3:1;

[0071] (2) Dialyze the reaction solution obtained in step (1) with a dialysis membrane (MW=3.5-8kDa) in ultrapure water with a pH value of 3.5 for 48 hours, and ...

Embodiment 3

[0074] In this example, to prepare HA-DOPA, the steps are as follows:

[0075] (1) Add dropwise the NHS solution with the concentration of 50mg / mL to the sodium hyaluronate (Mw=500kDa) aqueous solution with the concentration of 15mg / mL, then add the EDCI solution with the concentration of 150mg / mL dropwise, stir the reaction for 3h, add dropwise Dopamine hydrochloride aqueous solution with a concentration of 0.5mmol / L was stirred for 14 hours, and the pH value was controlled at 4.8 during the two stirring reactions. The operations of this step were all carried out under nitrogen protection. EDCI, NHS, dopamine hydrochloride and sodium hyaluronate The molar ratio of the carboxyl groups on is 1:2:2.5:1;

[0076] (2) Dialyze the reaction solution obtained in step (1) with a dialysis membrane (MW=3.5-8kDa) in ultrapure water with a pH value of 3.0 for 48 hours, and then vacuum freeze-dry to obtain HA-DOPA, and store the sample in a desiccator middle.

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Abstract

The invention provides a bone repair material allowing injection of a multi-pore structure and a preparation method of the bone repair material. The bone repair material comprises powder, a curing solution A and a curing solution B, wherein the powder is hydroxyapatite composite polylactic acid-glycolic acid copolymer porous microspheres, the curing solution A is a collagen solution, the curing solution B is a dopamine-hyaluronic acid aqueous solution, the powder is dispersed in the curing solution A and then uniformly mixed with the curing solution B, the pH value is adjusted to 7.5-8.5, thehydroxyapatite composite polylactic acid-glycolic acid copolymer porous microspheres and collagen are distributed in a 3D network structure of dopamine-hyaluronic acid copolymer hydrogel. The bone repair material has the multi-pore structure suitable for cell growth, can increase the tightness of combination of the bone repair material and autogenous bone and is expected to be applied to fields oftissue repair such as skin filling and beauty through adjustment of the composition proportion.

Description

technical field [0001] The invention belongs to the field of bone repair materials, and relates to an injectable bone repair material with multiple pore structures and a preparation method thereof. Background technique [0002] Injectable bone repair material is a kind of biomedical material used for local bone defect and repair. It can replace the traditional complex and time-consuming bone defect treatment methods, such as autologous bone graft and allogeneic bone graft. It has the advantages of small tissue damage, It has the advantages of simple operation and less surgical complications. Ideal injectable bone repair materials generally meet the following conditions: good biocompatibility, good osteoconductivity and osteoinductivity, certain pore size and porosity, degradable and absorbable in tissues, etc. [0003] At present, the clinically used bone defect repair materials mainly include polymethyl methacrylate (PMMA) and alloy materials. Although both have good mecha...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/50A61L27/52A61L27/54A61L27/12A61L27/18A61L27/24A61L27/20
CPCA61L27/12A61L27/18A61L27/20A61L27/24A61L27/50A61L27/52A61L27/54A61L27/56A61L2300/602A61L2400/06A61L2430/02C08L67/04C08L5/08
Inventor 孙勇樊渝江卢恭恭张兴栋王翔陈铁军
Owner SICHUAN UNIV
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