Biological efficient mixed protein feed as well as preparation method and application thereof

A protein feed and compound protein technology, applied in animal feed, animal feed, applications, etc., can solve the problems of poor antigenic protein effect, bitter taste and odor, and affect palatability, so as to reduce nutritional diarrhea and improve digestion and absorption The effect of increasing the ability and content of

Inactive Publication Date: 2018-12-18
圣百奥(山东)生物科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

The physical method has a good effect on the elimination of gossypol, but the effect on the antigenic protein is poor; the use of the chemical method will produce substance residues, which will have toxic and side effects on the body; the enzymatic method will produce bitterness and odor, which will affect the palatability; microbial fermentation method can effectively Removing the anti-nutritional factors in soybean meal, meanwhile microbial fermentation produces a variety of beneficial bacteria and aromatic odors, improving the palatability and absorption utilization of feed is a current research hotspot

Method used

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  • Biological efficient mixed protein feed as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Preparation method of bio-efficient mixed protein feed

[0044] 1) Preparation of composite protein materials. Take soybean meal (protein ≥46%) and cotton meal (protein ≥45%) in a mass ratio of 7:3, that is 700kg of soybean meal and 300kg of cotton meal, and stir with a stirrer (mixing time 5min, rotation speed 40-60 rpm) well mixed

[0045] 2) Preparation of compound fermentation broth. The strains for preparing the composite fermentation bacteria are Bacillus licheniformis, Lactobacillus plantarum, Saccharomyces cerevisiae, pectinase, mannanase and papain, and the mass ratio is 200:200:150:1000:500:1000. Take 200g of Bacillus subtilis, 200g of Lactobacillus plantarum, 150g of Saccharomyces cerevisiae, 1000g of pectinase, 500g of mannanase, and 1000g of papain, add to 100L of warm water at 35℃, add 2.5kg of sucrose, and use a blender (mixing time 25min, rotating speed 40-60 rpm) Stir and mix evenly to make compound fermentation broth.

[0046] 3) Inoculate comp...

Embodiment 2

[0049] Example 2: Comparative analysis of the nutritional value of bio-efficient mixed protein feed

[0050] 1 Test materials

[0051] The biological high-efficiency mixed protein feed obtained in Example 1 above is sample 1; three similar fermented or enzymatic protein products on the market are collected, sample 2, sample 3, and sample 4; untreated cotton meal is sample 5; unfermented protein content 46% of soybean meal products are sample 6.

[0052] 2 Test method

[0053] The above samples were tested for the crude protein, moisture, particle size, acid-soluble protein, protein solubility, volatile base nitrogen (TVB-N), glycinin and β-conglycinin content of the sample according to the following detection methods. The specific detection methods are as follows:

[0054] (1) Crude protein: according to GB / T 6432

[0055] (2) Moisture: according to GB / T 6435-2006

[0056] (3) Free gossypol: according to GB / T5009.148-2011

[0057] (4) Granularity: According to the regulations of GB / T 591...

Embodiment 3

[0080] Example 3. The effect of the product of the present invention instead of fish meal on the performance and economic benefits of weaned piglets

[0081] 1 Experimental design

[0082] Choose 180 piglets with similar parity and healthy initial weight of about 7.5kg Du Changda weaned piglets, divided into 3 groups, namely imported fishmeal group (Tianjin Yuanping International Trade Co., Ltd., imported fishmeal from Peru) and domestic fishmeal group (Shandong Rongcheng) The first fish meal factory) and the product group of the present invention, the premix is ​​the product of Jinan Ovipule Animal Nutrition Co., Ltd. (main ingredients: plasma protein powder 8%, glucose 3%, cooked soybean oil 0.2%, acidified milk replacer powder 30% , Zeolite powder 13.385%, whey powder 12%, hemoglobin powder 10%, peptin 0.1%, acidulant 2.4%, fragrance 0.2%, super sweetener 0.2%, 0.5% suckling pig core material 0.45%, Calcium hydrogen phosphate 8.5%, fine stone powder 6%, table salt 2.4%, 50% cho...

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Abstract

The invention relates to the technical field of preparation of biological feed and in particular relates to biological efficient mixed protein feed as well as a preparation method and application thereof. The method comprises the following steps: 1) preparing a compound protein substrate; 2) preparing a compound fermentation bacterium solution; 3) inoculating the bacterium solution obtained by step 2) into a compound protein raw material in step 1); 4) fermenting the compound protein raw material inoculated with the bacterium solution in step 3) to form fermented compound protein; 5) carryingout low-temperature drying on the fermented compound protein obtained by step 4) and crushing to form a biological efficient mixed protein feed finished product. According to the biological efficientmixed protein feed provided by the invention, a proper strain and a proper enzyme are compounded to carry out fermentation, and a low-temperature drying technology is adopted, so that the bioactivityof a metabolic product after fermentation is greatly improved, and the quantity of viable bacteria and the content of acid-soluble protein are about three times of that of common fermented protein type products; the content of glycinin and beta-conglycinin in the product is degraded by about 65 percent and is one time or more than one time of that of like products; the content of free gossypol islower than 500mg/kg and is degraded by 75 percent or more, and the capability of digesting and absorbing vegetable protein by animals can be remarkably improved.

Description

Technical field [0001] The invention relates to the technical field of biological feed preparation, in particular to a biological high-efficiency mixed protein feed and a preparation method and application thereof. Background technique [0002] Protein is an essential nutrient element for animal growth. Obviously, protein raw materials are the largest and one of the most important raw materials in animal diets. With the development of animal husbandry and feed industry, protein raw materials are gradually being standardized. At present, the most used vegetable protein source is soybean meal, and the animal protein source is fish meal. In terms of local resources, the quantities of soybean meal and fishmeal are very limited, and most of them rely on imports. This has brought great problems to China’s animal husbandry. China has a large population and limited arable land resources. In a short time, expanding the area of ​​arable land can solve the problem of vegetation. The lack...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/147A23K10/12
CPCA23K10/12A23K20/147
Inventor 葛龙王政陈帅胡红敏
Owner 圣百奥(山东)生物科技有限公司
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