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A kind of Acinetobacter for inducing tomato resistance to root-knot nematode incognita and its application

A technology for root-knot nematode incognita and Acinetobacter, applied in application, nematicide, seed and rhizome treatment, etc., to achieve excellent control effect, reduce usage, and protect the environment

Active Publication Date: 2021-08-13
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And utilize the metabolite of Acinetobacter to process tomato seed, the method for preventing and treating root-knot nematode incognita has not yet been reported

Method used

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  • A kind of Acinetobacter for inducing tomato resistance to root-knot nematode incognita and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Acinetobacter ( Acinetobacter guillouiae ) Culture of Sneb1988

[0013] Bacterial strain Acinetobacter of the present invention ( Acinetobacter guillouiae )Sneb1988 is prepared by culturing in a petri dish and then expanding fermentation. The specific method is as follows:

[0014] First for Acinetobacter ( Acinetobacter guillouiae ) Sneb1988 strain was fermented to prepare an Acinetobacter bacterial agent, and then the tomato inducing activity test was carried out on the bacterial agent to determine its inducing effect on tomato.

[0015] Inoculate the strain of Acinetobacter guillouiae Sneb1988 on the test tube medium. The medium formula is yeast mannitol agar (YMA), and the composition is (g / L): yeast extract 1.0; mannitol 10.0; hydrogen phosphate Dipotassium 0.5; Magnesium sulfate 0.2; Sodium chloride 0.1; Calcium carbonate 1.0; Agar 15g;

[0016] Then inoculate the test tube seed into a 250mL Erlenmeyer flask (150mL per bottle) liquid medium, t...

Embodiment 2

[0019] Embodiment 2: Acinetobacter ( Acinetobacter guillouiae ) Preparation of Sneb1988 bacterial agent

[0020] Acinetobacter ( Acinetobacter guillouiae ) The fermentation of Sneb1988 is basically the same as that in Example 1, except that the liquid-cultured strains are inoculated into a 30 L fermenter for fermentation, cultured at 28° C., pH 7.0, and fermented for 3 days.

Embodiment 3

[0021] Embodiment 3: Acinetobacter ( Acinetobacter guillouiae )Sneb1988 bacterial agent control field effect of root-knot nematode incognita

[0022] Field effect tests were carried out in Liqianhu Town, Tieling City, Liaoning Province and Dongbei River Greenhouse, Cainiu Township, Tieling County, Liaoning Province. The prepared fermentation stock solution was treated with seed coating with a volume ratio of 1:40 (that is, the treatment amount of the drug was 20 μL / g seed), dried under sterile conditions, and the treated tomato seeds were sown normally. Tomato plants can develop resistance to M. incognita at the seedling stage, and the induction effect is relatively stable, and a good control effect has been achieved.

[0023] 1. Preparation of test formulations

[0024] Acinetobacter was prepared by the aforementioned liquid fermentation culture method ( Acinetobacter guillouiae ) The bacterial agent of Sneb1988, the Acinetobacter concentration is 10 7 -10 8 CFU / mL;

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Abstract

The invention relates to an Acinetobacter that induces tomato resistance to root-knot nematode incognita. It is characterized in that: the Acinetobacter is stored in the General Microbiology Center of the China Microbiological Culture Collection Management Committee; the storage date is August 24, 2018 date; the deposit number is CGMCC No.16335. The bacteria agent of Acinetobacter has the purposes of inducing tomato resistance to M. incognita, and can induce tomato to produce resistance to M. incognita during seed germination and growth; the Acinetobacter proposed by the present invention has a control effect on nematodes , using this bacterium or the metabolites of this strain, tomato can be induced to produce resistance to root-knot nematode incognita during seed germination and growth, and the mixed use of Acinetobacter and nematicides has excellent control effect on root-knot nematode disease , can reduce the use of chemical pesticides, so as to achieve the purpose of protecting the environment.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, in particular to an Acinetobacter for inducing tomato resistance to root-knot nematode incognita and its application. Background technique [0002] In recent years, with the continuous expansion of vegetable production in greenhouse plastic sheds in my country, agriculture has been pushed to the height of factoryization. However, with a long planting period, heavy cropping, and pests and diseases seriously affect the sustainable development of vegetable production in protected areas. Among them, root-knot nematode is common and becoming more and more serious. Root-knot nematode is a typical soil-borne disease with a wide range of hosts, including more than 3,000 species of plants. Plants such as Solanaceae, Cucurbitaceae, and Brassicaceae are particularly affected, and it has become a worldwide problem in pest control. According to statistics, the losses caused by diseases caused b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P5/00A01C1/00C12R1/01
CPCA01C1/00A01N63/00C12N1/20C12N1/205C12R2001/01Y02A50/30
Inventor 陈立杰王帅段玉玺朱晓峰刘晓宇范海燕王媛媛赵劲捷
Owner SHENYANG AGRI UNIV