A lactic acid bacterium capable of degrading nitrite and biogenic amine and its application
A nitrite, biogenic amine technology, applied in the field of microorganisms, to achieve the effects of efficient degradation, good growth ability and acid production ability
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Embodiment 1
[0023] The preliminary screening of embodiment 1 lactic acid bacteria (Lactobacillus plantarum) LAB-H1
[0024] Pickle soups made by farmers were collected from Guangzhou, Chaoshan and other places, and the samples were diluted tenfold with sterile water (10 -1 、10 -2 、10 -3 、10 -4 times), take a gradient dilution sample and spread it evenly on the MRS modified agar medium: (peptone 10.0g / L, beef extract powder 8.0g / L, yeast extract powder 4.0g / L, glucose 20.0g / L, dipotassium hydrogen phosphate 2.0g / L, dipotassium hydrogen citrate 2.0g / L, sodium acetate 5.0g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.04g / L, Tween 801.0g / L, agar 15g / L, 20g / L L calcium carbonate, 0.2g / L bromocresol green), do three parallels for each gradient, culture at a constant temperature of 30°C, select a single colony of typical lactic acid bacteria colonies with calcium-dissolving circles, streak and purify on the plate, and passage Once, after microscopic examination as a pure species, combine...
Embodiment 2
[0030] Embodiment 2 Lactic acid bacteria LAB-H1 strain degrades nitrite experiment
[0031] The method that the ability of the lactic acid bacteria LAB-H1 degrading nitrite obtained by screening of the present invention is tested and verified is as follows:
[0032] Observe that the isolated lactic acid bacteria LAB-H1 contains 150μg / ml NaNO 2 The degradation of nitrite in MRS medium and simulated sausage fermentation condition medium, according to the inoculum size of 1:1000, the concentration was 2×10 6 CFU·ml -1Lactic acid bacteria were inoculated into the two mediums, cultured at 30°C for 48 hours, and the nitrite concentration was measured every 12 hours. The nitrite content is detected by the naphthalene ethylenediamine hydrochloride method in GB5009.33-2010. Before this, the standard curve is drawn with nitrite. The results are shown in Table 1. It can be seen that in the MRS culture medium After 48 hours, the nitrite degradation rate of lactic acid bacteria LAB-H1 r...
Embodiment 3
[0033] Example 3 Lactic acid bacteria LAB-H1 strain degradation biogenic amine experiment
[0034] The method that the ability of the lactic acid bacteria LAB-H1 that the present invention screens to obtain to degrade biogenic amines is tested and verified is as follows:
[0035] The screened lactic acid bacteria (Lactobacillus sp.) LAB-H1 were inoculated with seven biogenic amines (putrescine, cadaverine, tyramine, β-phenylethylamine, histamine, tryptamine, In the MRS medium (pH value 6.5) of spermidine, content is 100mg / ml respectively), cultivated at 30 ℃ for 24h, then centrifuged to get 1ml supernatant to carry out dansyl chloride derivatization, the specific method of derivation is: in 1ml sample Add 200 μl of 2mol / l sodium hydroxide solution to make it alkaline, then add 300 μl of saturated sodium bicarbonate solution for buffering, then add 1ml of derivatizing agent dansyl chloride solution (10mg / ml acetone), bathe in water at 40°C for 45min, in the dark Then add 100μl...
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