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A method for preparing microalgae oil by mixed culture

A technology of mixed culture and microalgae oil, which is applied in the field of biotechnology and bioenergy, can solve the problems of unfavorable algae absorption and utilization, no oil content involved, unfavorable microalgae growth, etc., so as to alleviate the greenhouse effect and waste gas pollution, and inhibit miscellaneous Bacterial growth, enhanced tolerance and carbon sequestration efficiency

Active Publication Date: 2022-03-04
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
[0005] But in practical application, when the environment CO 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low
At the same time, general microalgae are suitable for growth under neutral conditions, and are not conducive to the growth of microalgae under acidic or alkaline conditions, and microalgae use CO 2 Usually HCO dissolved in water 3 - It exists in the form of ions, and the solubility of carbon dioxide is low in a neutral environment, which is not conducive to the absorption and utilization of algae
Moreover, if the fossil fuel waste gas passed through contains high concentrations of NOx and other gases, it will also inhibit the growth of microalgae and reduce the efficiency of carbon sequestration

Method used

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  • A method for preparing microalgae oil by mixed culture
  • A method for preparing microalgae oil by mixed culture
  • A method for preparing microalgae oil by mixed culture

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preparation example Construction

[0022] The preparation process of the microalgae seed solution of the present invention is as follows: the microalgae culture adopts BG11 medium, and the formulation of the medium is shown in Table 1 and Table 2.

[0023] Table 1 BG11 medium

[0024]

[0025] *Table 2 Composition of A5+Co solution in Table 1

[0026]

[0027] First, prepare BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for cultivating Pseudochlorella kesserii FSH-Y3 and Scenedesmus obliquus FSH-Y2 to 10, and adjust the pH of the medium for cultivating Chlorella keserii SF-B1 The pH of the medium was adjusted to 8.0, and then Pseudochlorella kescherei FSH-Y3, Scenedesmus obliquus FSH-Y2, and Chlorella SF-B1 were inoculated in the above-mentioned medium respectively. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture until the...

Embodiment 1

[0029]The prepared microalgae seed liquids were respectively inoculated in BG11 medium to produce microalgae oil. In a 20L air-lift photobioreactor, the seed liquid of Pseudochlorella kesperi FSH-Y3 and the seed liquid of Chlorella SF-B1 were inoculated according to the volume ratio of 1:1, and the total inoculation amount of 20% was inoculated to In the BG11 medium, the pH value is controlled between 8 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 20°C, and the light cycle is 24h. The light to dark time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is measured by the n-hexane:ethyl acetate method The total lipid c...

Embodiment 2

[0034] The prepared microalgae seed liquids were respectively inoculated in BG11 medium to produce microalgae oil. In a 20L airlift photobioreactor, Scenedesmus obliquus FSH-Y2 seed liquid and Chlorella SF-B1 seed liquid were inoculated at a ratio of 1:1 by volume, and were inoculated to BG11 at a total inoculum of 20%. In the medium, the pH value is controlled between 8 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the cultivation temperature is 20°C, the light cycle is 24h, and the light is dark. The time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is measured by the n-hexane:ethyl acetate method The total lipid content, the results are ...

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Abstract

The invention relates to a method for preparing microalgae oil by mixed culture, adding microalgae culture medium and mixed microalgae seed liquid into a photobioreactor, maintaining the pH of the culture system at 8-12, preferably 9-11, and feeding CO with a volume content of 5v% to 45v% 2 , preferably 10v% to 30v%; wherein the mixed microalgae include Chlorella ( Chlorella sp. ) SF‑B1, which also includes Pseudochlorella kesperii ( Parachlorella kessleri ) FSH‑Y3 or / and Scenedesmus obliques ( Scenedesmus obliqnus ) FSH‑Y2. The inventive method has improved microalgae culture system to high concentration CO 2 The tolerance and solubility of microalgae improve the efficiency of carbon fixation, and the yield of microalgae oil is significantly increased, which is conducive to the production of biodiesel.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for preparing microalgae oil by mixed culture. Background technique [0002] As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. The fatty acids obtained from microalgae can be converted into fatty acid methyl ester, that is, biodiesel. Therefore, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12P7/6463C12P7/649C12R1/89
CPCC12P7/64C12P7/6463C12P7/649Y02E50/10
Inventor 师文静王鹏翔李晓姝孙启梅张霖樊亚超
Owner CHINA PETROLEUM & CHEM CORP
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