Preparation method and use method of dust explosion suppressor based on microbial capsule
A technology of microorganisms and anti-explosion agents, which is applied in the field of preparation of dust anti-explosion agents, can solve the problems of discounting anti-explosion effects, not taking into account, and enhancing the chemical activity of combustible dust, so as to achieve the protection of the ecological environment, avoid waste of resources, The effect of promoting the wrapping effect
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Embodiment 1
[0031] Taking Bacillus sphaericus as an example, the present invention is exemplified based on the preparation method of dust explosion suppressing agent of microbial capsule:
[0032] a Bacillus sphaericus is cultured, and the process of calcium carbonate crystal deposition induced by Bacillus sphaericus is regulated; the specific regulation process is: take LB nutrient broth to prepare 1300mL liquid medium, and 1200mL of it is evenly distributed in 6 cones In the bottle, the pH was adjusted to 7, 8, 9, 10, 11 and 12 in turn, and the concentration of the broth nutrient solution was adjusted to 5g / L, 10g / L, 15g / L, 20g / L, 25g / L and 30g / L respectively , after sterilization, inoculate on the ultra-clean workbench, the inoculation amount is 1% of the volume of the culture medium, place it in a shaking incubator at 28°C for shaking culture, and use a microplate reader to detect the number of microorganisms after 24 hours, the wavelength used for detection is 600nm, The measured val...
Embodiment 2
[0038] The difference from Example 1 is that in step d, the Bacillus sphaericus spores are added in a mass ratio of 2:1 to the Bacillus sphaericus spore sludge.
Embodiment 3
[0040] The difference from Example 1 is that in step d, the Bacillus sphaericus spores are added in a mass ratio of 10:1 to the Bacillus sphaericus spore sludge.
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