The invention discloses a preparation method and a use method of a dust explosion suppressor based on a microbial capsule, and belongs to the technical field of preparation of the dust explosion suppressor. The method includes the steps that firstly, carbonate mineralization bacteria is subjected to optimization culture, bacteria with high urease activity are selected to be prepared into hypopus spores, meanwhile, colloid bacillus hypopus spores are added, then a nutritional substrate, calcium chloride and urea are selected as core material filler, the double-strain spores and medium filler are mixed together, the mixture is subjected to extrusion forming through a pelletizer, and a core material component is formed. Porous diatomite is selected as a wall material, the core material component is adsorbed by the porous diatomite, and the micro-capsule is formed. The microbial capsule is spread in accumulated dust, the microbial capsule wall material is stressed and triggered to releasethe carbonate mineralization bacteria, urea is decomposed by the mineralization bacteria under the in-vivo urease effect, carbonate is generated, the carbonate is continuously chelated with calcium ions, and calcium carbonate is formed. The micro-capsule prepared with the method has a good explosion suppression effect, and environmental pollution can be reduced to a maximum degree.