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A moisturizing face cream containing tremella exopolysaccharide

An extracellular polysaccharide, moisturizing surface technology, applied in the field of skin care products, can solve the problems of time-consuming, labor-intensive, low efficiency, and limited polysaccharide application.

Active Publication Date: 2021-09-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when polysaccharides are extracted from the fruiting body of Tremella fungus for utilization, on the one hand, it takes a long time to grow into the fruiting body of Tremella fungus; Useful polysaccharides are not only costly, time-consuming and laborious, but also extremely low in efficiency, which limits the application of Tremella fruiting body polysaccharides
Moreover, and there are few studies on tremella exopolysaccharides at present, there is a lot of room for exploration

Method used

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  • A moisturizing face cream containing tremella exopolysaccharide
  • A moisturizing face cream containing tremella exopolysaccharide
  • A moisturizing face cream containing tremella exopolysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Screening of fungus of Bacillus sp embodiment

[0062] From six fuciformis Tyc63, Y2, Y11, Tr01, Tr21, Tr9901 best screened strains.

[0063] A, fungus spores and the culture rejuvenation

[0064] (1) stored at -80 ℃ refrigerator strains were crossed or coated tablet in a manner seeded on PDA solid medium in activation, 2-3d after incubation at 25 deg.] C in an incubator;

[0065] (2) selection medium well on single colony morphology, in a clean bench, a ring with an inoculating loop dipped inoculated PDB medium, 150r / min, 25 ℃ shake flask culture to produce a logarithmic growth phase 3d . After taking out at 4 ℃ spare.

[0066] Second, molecular identification of fuciformis

[0067] 1. Preparation of the PCR template DNA identification

[0068] (1) from the cells on a shaker fuciformis grown to log phase bacteria liquid at room temperature, at 8000r / min centrifugal 15min, the supernatant was discarded, to collect the liquid shake flask;

[0069] (2) collectin...

Embodiment 2

[0100] Example 2 determined high extracellular production of extracellular polysaccharide fuciformis Strains and fermentation period

[0101] An outer high producing extracellular polysaccharides fuciformis Strains

[0102] 1, extracellular polysaccharide extracted fuciformis, isolated

[0103] Example 1 determined Tyc63, Tr01, Tr21, Tr9901 these four strains is fuciformis, Tremella therefore Bacillus bacteria which four objects screening, specific experimental procedure is as follows:

[0104] Fuciformis broth → → centrifuged supernatant was concentrated and absolute ethanol was added 3V polysaccharide precipitation → → crude polysaccharide precipitate was dissolved by heating in distilled water → → → centrifugation supernatant protein removal method sevage → → → 3V of ethanol centrifuged and the supernatant The precipitate polysaccharide precipitation → → → freeze-dried purified polysaccharide

[0105] Steps:

[0106] (1) fuciformis fresh fermentation broth in 8000r / min centri...

Embodiment 3

[0134] Example 3 Antioxidant Activity fuciformis embodiment exopolysaccharide Analysis

[0135] 1, the removing effect fuciformis exopolysaccharide hydroxyl radical

[0136] Referring Yan Jun's experimental measurement (Yan Jun et al., 2005) in the reaction system containing 8.8mmol / L H 2 O 2 1mL, 9mmol / L FeSO 4 1mL, 9mmol / L of salicylic acid - ethanol 1mL, 1mL was added to final concentrations of polysaccharides of different H 2 O 2 Start the reaction, 37 [deg.] C the reaction 0.5h, distilled water as reference, the absorbance was measured at each concentration at 510nm. Considering the light absorption values ​​of the polysaccharide itself to 9mmol / L FeSO 4 1mL, 9mmol / L of salicylic acid - ethanol 1mL, the polysaccharide solution 1mL 1mL distilled water and different concentrations of the polysaccharide as the background absorption value.

[0137] Clearance rate is calculated as follows:

[0138]

[0139] Where: A 0 The absorbance of the control solution; A X Is the ...

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Abstract

The invention discloses a moisturizing face cream containing white fungus spore exopolysaccharide. The preparation process of the Tremella spore exopolysaccharide is as follows: take the supernatant of the Tremella spore fermentation liquid, add absolute ethanol for precipitation; then dissolve the precipitate in water, take the supernatant to remove protein by Sevage method, and centrifuge to obtain the supernatant , and then add ethanol for precipitation, and the exopolysaccharide of Tremella spores can be obtained after the precipitate is freeze-dried. The present invention uses the fermented liquid of tremella spores as raw material, separates and extracts exopolysaccharide with moisturizing effect therefrom, and the purified exopolysaccharide has good moisturizing effect, and even the moisturizing effect is better than glycerin; meanwhile, it also has good moisturizing effect Antioxidant effect, the exopolysaccharide can be used as a moisturizing active ingredient and an antioxidative active ingredient to prepare skin care products; the prepared skin care product not only has a good moisturizing effect, but also has the function of repairing cell damage caused by UVB radiation.

Description

Technical field [0001] The present invention relates to the field of skin care products, and more particularly, to a moisturizing cream containing fuciformis exopolysaccharides. Background technique [0002] A class of Tremella polysaccharide is extracted from the fruiting bodies of the fungus, spores submerged fermentation liquid or the like, to give the heteropolysaccharide, according to the nature and origin be divided into neutral polysaccharides, acid polysaccharides, gellan, cell wall polysaccharides and acidic oligomerization five kinds of sugar, can promote immune function, inhibition of tumor deterioration, reducing blood glucose and lipid content, slow clearance of free radicals the body ages, and also serves to protect the body against radiation damage. [0003] However, when using the polysaccharide extracted from the fruiting bodies of the fungus, on the one hand due to the fungus grown fruiting body takes a long time; on the other hand, Tremella polysaccharide conte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/06A61K8/73A61K8/9728A61Q19/00A61Q19/08
CPCA61K8/73A61K2800/85A61Q19/00A61Q19/004A61Q19/08A61K8/9728
Inventor 林俊芳郑倩望王久莹郭丽琼
Owner SOUTH CHINA AGRI UNIV
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