Method and strain for remediating arsenic polluted high-salinity alkaline water through Synechocystis sp. in situ

An in-situ remediation and saline-alkali water technology, applied in the direction of microorganism-based methods, water pollutants, chemical instruments and methods, etc., can solve the problems of arsenic desorption, secondary release of arsenic, etc. High adaptability and ideal treatment effect

Active Publication Date: 2019-08-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

The previous research and application of microbial remediation to remediate arsenic pollution in surface water mainly achieved the fixation and remediation of arsenic pollution in water through the ion exchange, complexation and coordinatio

Method used

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  • Method and strain for remediating arsenic polluted high-salinity alkaline water through Synechocystis sp. in situ
  • Method and strain for remediating arsenic polluted high-salinity alkaline water through Synechocystis sp. in situ

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Embodiment 1

[0026] The separation and identification of embodiment 1 cyanobacteria

[0027] 1. Separation of cyanobacteria: Separation of cyanobacteria from arsenic-contaminated water body soil, sediment, water and algae biofilm in saline-alkali areas.

[0028] (1) Cultivation: Weigh 5g (wet weight, no drying) of soil and sediment removed from impurities and add them to a 250mL Erlenmeyer flask, add 50ml of water samples from Xinjiang Kuitun 123 group alkali drainage canal (salinity 15‰, pH8 .24), then add 100mL of sterilized NaCl with a final concentration of 5‰ (5g / L) and a final concentration of 5mg / L NaAsO 2 and 36mg / L CaCl 2 2H 2 The BG11 liquid culture medium of O was cultivated under the conditions of temperature of 25°C, light intensity of 2000lux, and light / darkness of 12h / 12h. When algae grow in the culture solution, the culture solution is mixed with sterile water by 10 -1 、10 -2 、10 -3 , 10 -4 、10 -5 、10 -6 Concentration gradient dilution, use a coating bar to coat in...

Embodiment 2

[0045] (1) Preparation of cyanobacteria: Inoculate cyanobacteria CCTCC NO: M 2019135 into 500ml containing 36mg / L CaCl 2 2H 2 In the Erlenmeyer flask of the BG11 liquid culture medium of O, and be placed on 25 ℃, light intensity 2000lux, light / darkness are in 12 / 12 environment, cultivate to logarithmic growth phase, obtain culture fluid (cyanobacteria OD 685 =0.7), the culture solution was centrifuged at 5500rpm for 20min, and after the supernatant was removed, the bacterial cell precipitate was collected, which was the cyanobacterial cell. Take a small amount of thalline precipitation, fix the cells with 2.5% aqueous solution of pentanediol with a mass concentration, after dehydrating with ethanol, dry in a freeze dryer (-80°C, vacuum environment) for 48h, take it out, and observe the cyanobacteria thallus by SEM, as figure 1 .

[0046] (2) Restoration of simulated water samples polluted by high salinity and alkali arsenic: BG11 liquid culture medium added with 25g / L NaCl a...

Embodiment 3

[0050]The NaCl concentration is adjusted to 35g / L in embodiment 2, other is the same as embodiment 1, carries out repair and the inspection of stability, the result shows, through laboratory simulation arsenic polluted water experiment, be 35g / L in salt content, different arsenic pollution Level (10mg / L, 20mg / L, 40mg / L) of the water environment, using cyanobacteria CCTCC NO: M 2019135, after 14 days of in-situ restoration, the removal rate of arsenic reached 73.67%, 74.84%, 73.48% respectively . After the stability detection of arsenic pollution in the laboratory, in a 35g / L saline environment, using the simulated arsenic-contaminated water sample repaired by the described cyanobacteria, after evaporation and redissolving, different arsenic pollution levels (10mg / L, 20mg / L, 40mg / L) still maintained a good removal rate, respectively: 51.02%, 47.17%, 45.71%.

[0051] According to the method of the present invention, through laboratory simulation of high salinity arsenic contam...

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Abstract

The invention discloses a method and a strain for remediating arsenic polluted high-salinity alkaline water through Synechocystis sp. in situ. The Synechocystis sp. can survive in the environment withthe highest salinity of 45 permillage, the rate of average growth biomass of 4 days is 0.104965 per day on the basis of absorbance value of OD685, the average photosystem activity Fv/Fm value of 4 days is 0.3355, the Synechocystis sp. can grow in the environment with the highest arsenic content of 50 mg/L at the average growth rate of 0.134 per day, and the average photosystem activity Fv/Fm value of 4 days is 0.3241675. The arsenic-containing high-salinity wastewater is treated by Synechocystis sp. without heterotopia, the method is convenient, low in cost and more ideal in treatment effect,the removal rate of arsenic can reach 70% or above, and secondary release is not easily caused. The Synechocystis sp. used in the method has the characteristics of high environment adaptability and capability of surviving in the severe environment. Therefore, the remediation technology is stable and environmentally friendly.

Description

[0001] (1) Technical field [0002] The invention relates to a new strain of cyanobacteria and a method for in-situ restoration of arsenic-polluted high-salt-alkali water by using cyanobacteria. [0003] (2) Background technology [0004] Arsenic is a metalloid that exists widely in nature, and it is also a pollutant that is extremely toxic to the human body. Studies have shown that the human body's arsenic poisoning dose is 0.01-0.052g, and the lethal dose is 0.06-0.02g. With the continuous development of industry and agriculture, arsenic, as an inevitable pollutant, is easily released into the environment, making animals, plants and even humans in the environment easily exposed to arsenic pollution, especially in arid / semi-arid areas. , In areas with high salinization of soil and water, due to the environmental characteristics of these areas - high salinity, arsenic is highly mobile, so the arsenic pollution in the environment of these areas is often serious. According to t...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C02F101/10C12R1/01
CPCC02F3/34C02F2101/103C12N1/20C12N1/205C12R2001/01
Inventor 张道勇祝鹏烽潘响亮
Owner ZHEJIANG UNIV OF TECH
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