cuo-polylysine/graphene electrode and its preparation method and application

A technology of polylysine and graphene, applied in the direction of material electrochemical variables, material analysis through electromagnetic means, instruments, etc., can solve the problems of immature melatonin and pyridoxine, etc., to achieve the benefit of civilian use, The production process is simple and the detection limit is low

Active Publication Date: 2021-01-29
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current research on the simultaneous detection of melatonin and pyridoxine based on electrochemical methods is still immature.

Method used

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  • cuo-polylysine/graphene electrode and its preparation method and application
  • cuo-polylysine/graphene electrode and its preparation method and application
  • cuo-polylysine/graphene electrode and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0067] A preparation method of CuO-polylysine / graphene / substrate electrode, comprising the following steps:

[0068] 1) Prepare graphene / substrate electrodes, including ①~③, as follows:

[0069] ① Grind the 2×1×0.1 (length×width×thickness, cm) substrate with sandpaper to remove the oxide on the surface of the substrate, cleaning: Ultrasonic cleaning in the order of ultrapure water-ethanol-ultrapure water for 5 minutes each , drying with an infrared lamp, wherein the substrate is a tantalum sheet;

[0070] ② Drop 200 μL of Ni(NO 3 ) 2 ethanol solution, and baked under infrared light for 2 minutes for drying, wherein, Ni(NO 3 ) 2 Ethanol solution is composed of ethanol and Ni(NO 3 ) 2 ·6H 2 O is uniformly mixed, Ni(NO 3 ) 2 Ni(NO 3 ) 2 ·6H 2 The concentration of O is 0.6M.

[0071] ③ Perform DC plasma jet chemical vapor deposition on the substrate obtained in step ②: polish the reaction chamber and sample stage (Mo stage) of the DC plasma jet chemical vapor depositi...

Embodiment 2

[0087] CuO-polylysine / graphene / substrate electrode to detect melatonin alone:

[0088] a) Prepare multiple modeling solutions, each of which is a PBS buffer solution mixed with melatonin, the concentration of melatonin in multiple modeling solutions is different, CuO-polylysine in Example 1 The three-electrode system composed of / graphene / substrate electrode, reference electrode and counter electrode was respectively inserted into the modeling solution, and the current-voltage curve of each modeling solution was obtained by differential pulse voltammetry (DPV), as shown in Figure 5 As shown, among them, the potential of differential pulse voltammetry is set to -0.6 ~ 1.2V; the reference electrode is a saturated calomel electrode, and the counter electrode is a platinum sheet electrode.

[0089] b) Current-voltage curves for each modeled solution:

[0090] For the modeling solution containing melatonin: the potential corresponding to the peak at 0-0.2V is used as a qualitativ...

Embodiment 3

[0099] CuO-polylysine / graphene / substrate electrode for the detection of pyridoxine alone:

[0100] a) Prepare multiple modeling solutions, each of which is a PBS buffer solution mixed with pyridoxine, and the concentrations of pyridoxine in multiple modeling solutions are different. CuO-polylysine in Example 1 The three-electrode system composed of / graphene / substrate electrode, reference electrode and counter electrode was respectively inserted into the modeling solution, and the current-voltage curve of each modeling solution was obtained by differential pulse voltammetry (DPV), as shown in Figure 7 As shown, among them, the potential of differential pulse voltammetry is set to -0.6 ~ 1.2V; the reference electrode is a saturated calomel electrode, and the counter electrode is a platinum sheet electrode.

[0101] b) Current-voltage curves for each modeled solution:

[0102] For pyridoxine: the potential corresponding to the peak at 0.6-0.8V is used as a qualitative index, a...

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Abstract

The invention discloses a CuO-polylysine / graphene / substrate electrode and a preparation method and an application thereof. The preparation method of the CuO-polylysine / graphene / substrate electrode includes the following steps: preparing a graphene / substrate electrode, inserting the graphene / substrate electrode and a counter electrode into an electrolyte, selecting a pulse wave to perform electrolytic deposition on the graphene / substrate electrode to obtain Cu-polylysine / graphene / substrate electrode, inserting the Cu-polylysine / graphene / substrate electrode, the counter electrode and a referenceelectrode into a sodium hydroxide aqueous solution, and performing oxidation treatment on Cu by utilizing cyclic voltammetry A to obtain the CuO-polylysine / graphene / substrate electrode. A manufacturing process of the CuO-polylysine / graphene / substrate electrode is simple, and is convenient to operate; an electrode can be modified several times by an electrochemical method; reproducibility, repeatability and stability of a sensor are good, a detection limit is low, and test sensitivity and accuracy are high; and the cost is low, and the electrode is beneficial to civilian use.

Description

technical field [0001] The invention belongs to the technical field of current sensors, and in particular relates to a CuO-polylysine / graphene / substrate electrode and its preparation method and application. Background technique [0002] Melatonin is ubiquitous in the tissues and organs of plants, regulates the growth and development of plants, and enhances the tolerance of plants to stress. Therefore, melatonin can be used in agricultural production to improve the ability of plants to resist external biotic and abiotic stresses. Reduce the use of chemicals and pesticides to ensure crop yield and quality. [0003] Pyridoxine, namely vitamin B6 (VB6), can protect plants from environmental stresses such as low temperature, ultraviolet rays, oxidation, and osmotic pressure. In recent years, it has been found that VB6 is also an antioxidant, which can effectively quench singlet oxygen and superoxide anion free radicals. [0004] In view of the important regulatory effects of me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 李明吉刘翼李红姬李翠平钱莉荣杨保和
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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