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3D printing fibroin/gelatin support used for cartilage repairing, and preparation method thereof

A 3D printing and cartilage repair technology, applied in medical science, prosthesis, additive processing, etc., can solve the problems of unfavorable cell migration, adhesion and growth, no cell adhesion sites, poor mechanical properties of gelatin, etc., to achieve The effect of simple preparation process, easy source, high compressive modulus and compressive fatigue resistance

Inactive Publication Date: 2019-09-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of using gelatin as a cartilage repair material is that it is known that the site on the molecular chain can be used for cell recognition and adhesion, and it has good biocompatibility. However, gelatin also has defects such as poor mechanical properties and short degradation time in vivo.
On the contrary, silk fibroin, as a natural polymer extracted from silk, has good mechanical properties and a relatively controllable degradation rate, but there is no cell adhesion site on the natural silk fibroin molecular chain, which is not conducive to cell adhesion. Migration, adhesion and growth on

Method used

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  • 3D printing fibroin/gelatin support used for cartilage repairing, and preparation method thereof
  • 3D printing fibroin/gelatin support used for cartilage repairing, and preparation method thereof
  • 3D printing fibroin/gelatin support used for cartilage repairing, and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Printing material preparation

[0034] 1.1) Preparation of tyramide-grafted modified gelatin: prepare 500ml of 50mM morpholineethanesulfonic acid buffer, add 10g of gelatin powder, stir at 50°C, and fully dissolve. Add 5g of tyramine hydrochloride, stir and dissolve fully; after the solution is cooled to room temperature, add carboxyl activator N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodi Imine hydrochloride 0.37g / 0.11g, to activate the carboxyl group on the gelatin molecular chain, react at room temperature for 12h; put the reaction product into a dialysis bag with a molecular weight cut-off of 10000-12000, and dialyze for 4 days under a deionized water environment. Finally, the water was removed by a freeze dryer to obtain a white spongy modified product, which was stored in a moisture-proof cabinet for future use.

[0035] 1.2) Preparation of silk fibroin solution: add 4g of degummed silk to a beaker, then add 20ml of 9.3mol / l lithium bromide...

Embodiment 2

[0041] 1) Printing material preparation

[0042]1.1) Preparation of tyramide-grafted modified gelatin: prepare 500ml of 50mM morpholineethanesulfonic acid buffer, add 10g of gelatin powder, stir at 50°C, and fully dissolve. Add 5g of tyramine hydrochloride, stir and dissolve fully; after the solution is cooled to room temperature, add carboxyl activator N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodi Imine hydrochloride 0.37g / 0.11g, to activate the carboxyl group on the gelatin molecular chain, react at room temperature for 12h; put the reaction product into a dialysis bag with a molecular weight cut-off of 10000-12000, and dialyze for 4 days under a deionized water environment. Finally, the water was removed by a freeze dryer to obtain a white spongy modified product, which was stored in a moisture-proof cabinet for future use.

[0043] 1.2) Preparation of silk fibroin solution: add 4g of degummed silk to a beaker, then add 20ml of 9.3mol / l lithium bromide ...

Embodiment 3

[0049] 1) Printing material preparation

[0050] 1.1) Preparation of tyramide-grafted modified gelatin: prepare 500ml of 50mM morpholineethanesulfonic acid buffer, add 10g of gelatin powder, stir at 50°C, and fully dissolve. Add 5g of tyramine hydrochloride, stir and dissolve fully; after the solution is cooled to room temperature, add carboxyl activator N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodi Imine hydrochloride 0.37g / 0.11g, to activate the carboxyl group on the gelatin molecular chain, react at room temperature for 12h; put the reaction product into a dialysis bag with a molecular weight cut-off of 10000-12000, and dialyze for 4 days under a deionized water environment. Finally, the water was removed by a freeze dryer to obtain a white spongy modified product, which was stored in a moisture-proof cabinet for future use.

[0051] 1.2) Preparation of silk fibroin solution: add 4g of degummed silk to a beaker, then add 20ml of 9.3mol / l lithium bromide...

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Abstract

The invention discloses a 3D printing fibroin / gelatin support used for cartilage repairing, and a preparation method thereof. The support takes silk fibroin, tyramine graft modified gelatin and horseradish peroxidase as slurry; the porous hydrogel support is constructed by utilizing a 3D printing technology; enzyme crosslinking can be realized through the immersion of hydrogen peroxide; and mechanical properties can be enhanced by further utilizing an alcoholic solution to process. The support combines the excellent characteristics of the gelatin and fibroin, so that the support can have goodbiocompatibility, mechanical properties and compression fatigue resistance, and can be applied to the regeneration and repairing of cartilage tissues.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a 3D printing silk fibroin / gelatin scaffold for cartilage repair and a preparation method thereof. Background technique [0002] Once the cartilage tissue is damaged, it is difficult to achieve self-repair. Although many techniques have been applied to the treatment of cartilage defects clinically, there are deficiencies in various therapies. The research and development of tissue engineered cartilage has brought new ideas for the repair of cartilage defects. Cartilage scaffold materials need to have good biocompatibility, degradation rate and mechanical properties that match cartilage tissue regeneration; there are many ways to construct cartilage tissue engineering scaffolds. Compared with some traditional scaffold molding methods, 3D printing can More precise control over the structure of the scaffold. Hydrogel has a structure and water content similar to human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/52A61L27/56B33Y10/00B33Y80/00
CPCA61L27/222A61L27/227A61L27/50A61L27/52A61L27/56A61L2430/06B33Y10/00B33Y80/00
Inventor 曹晓东徐晟戴旗远李庆涛
Owner SOUTH CHINA UNIV OF TECH
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