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Serum biomarker group for diagnosis of ischemic stroke complicated with stress ulcer and application thereof

A biomarker, ischemic stroke technology, applied in the biological field, can solve the problem of increasing gastrointestinal and respiratory tract infections in patients, and achieve the effect of improving treatment effect, good specificity and sensitivity, and improving patient prognosis

Active Publication Date: 2019-09-06
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method ignores the individual differences among patients, and it is easy to increase the probability of gastrointestinal and respiratory tract infections in patients

Method used

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  • Serum biomarker group for diagnosis of ischemic stroke complicated with stress ulcer and application thereof
  • Serum biomarker group for diagnosis of ischemic stroke complicated with stress ulcer and application thereof
  • Serum biomarker group for diagnosis of ischemic stroke complicated with stress ulcer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Screening Characterization of Serum Differential Metabolites Between IS Patients and ISSU Patients

[0032] 1. Objects and methods

[0033] 1. Sample collection and processing

[0034] The study was approved by the Hospital Ethics Committee and informed consent was signed by the patients or their families. Serum samples from 33 IS patients and 15 ISSU patients were collected and stored at -80°C until use. Take 20 μL from each sample to be tested, mix well to prepare a QC sample, and store it under the same conditions.

[0035] 2. Standards and reagents

[0036] Stable isotope-labeled standards D1-leucine (99%) and heptadecanoic acid (99.9%) were purchased from Sigma-Aldrich, USA, and D5-cholic acid (98%) was purchased from Cambridge Isotope Laboratories, USA. Dansyl chloride (Dns-Cl, content 98%) was purchased from Shanghai Bailingwei Company; dansylpiperazine (Dns-PP) was prepared according to the existing process. 2-(7-benzotriazole oxide)-N,N,N',N'-te...

Embodiment 2

[0066] Example 2: Construction of a ROC curve for verifying the ability of 6 differential metabolites to diagnose IS and ISSU

[0067] Receiver operating curve (ROC) method was used for verification, and the ability to diagnose and distinguish IS and ISSU patients was judged by the expression levels of differential metabolites in the serum of IS and ISSU patients. The results showed that the six differential metabolites, glycine, phenylalanine, ornithine, palmitoleic acid, arachidonic acid, and oleic acid, had a strong ability to diagnose and distinguish between IS and ISSU patients, and the area under the ROC curve ( AUC) are greater than 0.7, which has clinical diagnostic significance; when combined for diagnosis, with the increase of the number of combinations, the AUC further increases, and the highest when all 6 are combined, the AUC reaches 0.986, and the sensitivity and specificity are 93.3% and 93.9% respectively. %. See Tables 2-4 for single and any combination of 2 ...

Embodiment 3

[0076] Embodiment 3: the preparation of detection kit:

[0077] Based on the serum biomarkers provided by the present invention, a detection kit is prepared, which contains the following components:

[0078] Serum biomarker standards: glycine, phenylalanine, ornithine, palmitoleic acid, arachidonic acid, oleic acid;

[0079] Detection reagents: dansyl chloride, dansyl piperazine, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, ethyl acetate, acetone , acetonitrile, methanol, formic acid (for sample preparation, derivatization reaction and LC-MS / MS determination);

[0080] Internal standard: Stable isotope labeled standards D1-leucine, D5-cholic acid and heptadecanoic acid.

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Abstract

The invention discloses a group of serum metabolite micromolecule markers related to the diagnosis of ischemic stroke complicated with stress ulcer. The biomarkers include one or more of glycine, phenylalanine, ornithine, palmitoleic acid, arachidonic acid, and oleic acid. The analysis and detection method based on the biomarker group has advantages of high sensitivity, high throughput and simpleoperation; the accurate diagnosis of ischemic stroke complicated with stress ulcer can be realized; and a diagnostic reagent for the ischemic stroke complicated with stress ulcer can be prepared.

Description

[0001] technology neighborhood [0002] The invention belongs to the field of biotechnology, and in particular relates to a group of serum biomarker combinations for diagnosing ischemic stroke complicated by stress ulcer and application thereof. Background technique [0003] Ischemic stroke (IS), also known as cerebral infarction, occurs in the middle-aged and elderly people due to impairment of blood and oxygen supply to the brain, necrosis or softening of brain tissue, and secondary neurological dysfunction. IS has a high clinical mortality and disability rate. According to the "2018 China Health Statistical Summary", as many as 1.96 million patients die of stroke in my country every year, accounting for about 20% of the total death toll. a huge threat. Clinically, the reason for the high fatality rate of IS is that in addition to its dangerous condition, it is also prone to various complications, such as upper gastrointestinal bleeding, lung infection, and epilepsy. Stress...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/067
Inventor 黄寅张尊建许风国杨旭萍
Owner CHINA PHARM UNIV
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