Application of serum biomarkers in the preparation of diagnostic reagents for issu

A biomarker and serum technology, applied in the field of serum biomarker combinations, can solve the problems of increasing gastrointestinal and respiratory tract infections in patients, and achieve the effect of improving treatment effect, improving patient prognosis, and stabilizing results

Active Publication Date: 2021-09-28
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method ignores the individual differences among patients, and it is easy to increase the probability of gastrointestinal and respiratory tract infections in patients

Method used

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  • Application of serum biomarkers in the preparation of diagnostic reagents for issu
  • Application of serum biomarkers in the preparation of diagnostic reagents for issu
  • Application of serum biomarkers in the preparation of diagnostic reagents for issu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening characterization of serum differential metabolites between IS patients and ISSU patients

[0031] 1. Objects and Methods

[0032] 1. Sample collection and processing

[0033] The study was approved by the hospital ethics committee, and the patients or their families signed informed consent. Serum samples from 33 IS patients and 15 ISSU patients were collected and stored at -80°C for cryopreservation. Take 20 μL from each sample to be tested, mix well to prepare QC samples, and store them under the same conditions.

[0034] 2. Standards and Reagents

[0035] Stable isotope-labeled standards D1-leucine (content 99%) and heptadecanoic acid (content 99.9%) were purchased from Sigma-Aldrich Company in the United States, and D5-cholic acid (content 98%) was purchased from Cambridge Isotope Laboratories Company in the United States. Dansyl chloride (Dns-Cl, content 98%) was purchased from Shanghai Bailingwei Company; dansylpiperazine (Dns-PP) was prepa...

Embodiment 2

[0065] Example 2: Construction of a ROC curve for verifying the ability of 6 differential metabolites to diagnose IS and ISSU

[0066] Receiver operating curve (ROC) method was used for verification, and the ability to diagnose and distinguish IS and ISSU patients was judged by the expression levels of differential metabolites in the serum of IS and ISSU patients. The results showed that the six differential metabolites, glycine, phenylalanine, ornithine, palmitoleic acid, arachidonic acid, and oleic acid, had a strong ability to diagnose and distinguish between IS and ISSU patients, and the area under the ROC curve ( AUC) are greater than 0.7, which has clinical diagnostic significance; when used in combination for diagnosis, with the increase of the number of combinations, the AUC further increases, the highest when all 6 are combined, the AUC reaches 0.986, and the sensitivity and specificity are 93.3% and 93.9% respectively %. See Tables 2-4 for single and any combination...

Embodiment 3

[0075] Embodiment 3: the preparation of detection kit:

[0076] Based on the serum biomarkers provided by the present invention, a detection kit is prepared, which contains the following components:

[0077] Serum biomarker standards: glycine, phenylalanine, ornithine, palmitoleic acid, arachidonic acid, oleic acid;

[0078] Detection reagents: dansyl chloride, dansyl piperazine, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, ethyl acetate, acetone , acetonitrile, methanol, formic acid (for sample preparation, derivatization reaction and LC-MS / MS determination);

[0079] Internal standard: Stable isotope labeled standards D1-leucine, D5-cholic acid and heptadecanoic acid.

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Abstract

The invention discloses a group of serum metabolic small molecular markers related to the diagnosis of ischemic stroke complicated by stress ulcers. The biomarkers include glycine, phenylalanine, ornithine, palmitoleic acid, arachidose One or more of alkenoic acid and oleic acid. The analysis and detection method based on this group of biomarkers has the advantages of high sensitivity, high throughput, and easy operation. It can realize the accurate diagnosis of ischemic stroke complicated by stress ulcer, and can be applied to the preparation of ischemic stroke complicated by stress ulcer. Diagnostic reagents for sexual ulcers.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a combination of serum biomarkers for diagnosing ischemic stroke complicated with stress ulcer and application thereof. Background technique [0002] Ischemic stroke (IS), also known as cerebral infarction, is caused by obstruction of blood supply and oxygen supply to the brain, necrosis or softening of brain tissue, and secondary neurological dysfunction, mostly in middle-aged and elderly people. IS has a high mortality and morbidity rate in clinical practice. According to the "2018 China Health Statistics Summary", the number of stroke patients in my country is as high as 1.96 million each year, accounting for about 20% of the total number of deaths. a huge threat. In clinical practice, the reason for the high fatality rate of IS is that, in addition to its own dangerous condition, it is prone to a variety of complications, such as upper gastrointestinal bleeding, pulmo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/067
Inventor 黄寅张尊建许风国杨旭萍
Owner CHINA PHARM UNIV
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