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Microbial agent capable of effectively governing uranium pollution and application of microbial agent

A technology for microbial inoculum and uranium contamination, applied in the field of radioactive contamination bioremediation, can solve the problems of increased difficulty, easy shedding of fixation, long acquisition period, etc., and achieves the effects of improving mineralization capacity, significant treatment effect, and short restoration period

Active Publication Date: 2019-09-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In related fields, most of the technologies for heavy metal remediation do not include the remediation of uranium pollution. For example, the patent document "A Biochemical Composite Soil Heavy Metal Pollution Fixing Adsorbent and Its Application" (application number: 201610488744.1, publication date: 2016.11 .23) A biochemical method is disclosed to remediate heavy metal pollution in soil. Its working principle is to add photosynthetic bacteria, actinomycetes, yeast, iron powder, zeolite, mercapto chitosan and other substances to the soil, and enhance the Adsorption of cadmium, chromium, copper, mercury, arsenic, and lead, but the patent document does not indicate whether this method has adsorption effect on uranium. If the method is directly used for the adsorption of uranium, since the adsorption is usually accompanied by With desorption reaction, this method is also prone to shedding phenomenon for the fixation of uranium, resulting in the re-migration of uranium.
In addition, there are also a small number of patents that mention the restoration of heavy metals by Bacillus thuringiensis, such as the patent document "A microbial product and its manufacturing method for simultaneously controlling heavy metal pollution and insect damage" (application number: 201610713954.6, publication date: 2017.01.04) , its working principle is that Brevibacillus lateralsporosa and Bacillus thuringiensis act as insecticides, Bacillus subtilis decomposes the necrotic roots of crops to increase soil fertility, and other microorganisms are used to enrich heavy metals in the soil. This patent document is aimed at Dry soil polluted by heavy metals in agriculture, but the ability of Bacillus thuringiensis to mineralize hexavalent uranium has not been involved, let alone its effect on the restoration and treatment of uranium-contaminated water bodies
The patent document "A Method for Treating Heavy Metals in Soil" (application number: 201610250782.3, publication date: 2016.07.27) discloses a microbial agent to treat 8 kinds of pollution in soil, including cadmium, mercury, arsenic, copper, lead, chromium, nickel and zinc. method, but it does not involve the treatment of uranium pollution. In addition, the acquisition of its beneficial bacteria must first be implanted in plants, and then obtained from cow dung for use by cattle. The acquisition period is long and the operation is complicated. Increased the difficulty of practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Affected by uranium mining, the water body near a uranium mine in Xinjiang is seriously polluted by uranium, and the microbial preparation of the present invention is used for repair through the following steps:

[0029] (1) Detection: Take an appropriate amount of uranium-contaminated water body samples, use ICP-MS to detect the uranium concentration in the water body is 8.5mg / L, calculate the required amount of bacteria according to the volume of the repaired water body, and ensure that the amount of bacteria is 1g / L.

[0030] (2) pH adjustment: the pH of the water body is detected to be 4, and the pH is adjusted to 5-6 with NaOH solution.

[0031] (3) Mixing: Mix Bacillus thuringiensis, Bacillus cereus and Saccharomyces cerevisiae in a weight ratio of 4:3:3 evenly. Three kinds of bacterium can be purchased directly in China General Microorganism Culture Collection Management Center (CGMCC), adopt Bacillus thuringiensis (Bacillus thuringiensis 016), Bacillus cereus (B...

Embodiment 2

[0037] Firstly, Bacillus thuringiensis, Bacillus cereus and Saccharomyces cerevisiae were activated by culturing in a shaker at 30°C for 12 hours at a shaking speed of 160 rpm, and then transferred to 100 mL of LB medium for 24 hours respectively. Afterwards, the bacteria were collected by centrifugation at 6000 rpm, and washed three times with sterilized 1 mM NaCl solution to wash away the residual medium components, and three kinds of bacteria were collected.

[0038] (1) Mix the collected three kinds of bacteria according to the weight ratio of 5:4:2, and resuspend them in a certain volume of U(VI) solution (the concentration of uranium is 100mg / L, 200mg / L, 300mg / L), so that the bacterial cell concentration is 1g / L (dry weight). Then place it in a 30°C, 160rpm constant temperature shaker to react, take samples every 24 hours to measure the remaining uranium concentration in the solution, centrifuge to collect the precipitate, wash it with sterilized distilled water for 3 ti...

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PUM

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Abstract

The invention provides a microbial agent capable of effectively governing uranium pollution and an application of the microbial agent. The microbial agent consists of bacillus thuringiensis, bacillus cereus and saccharomyces cerevisiae. The strains are in organic cooperation, so that the mineralization capacity on uranium is increased, the time of minerals having good crystal form is shortened, and besides, the microbial agent can still exert effects in the environment very high in uranium concentration or other extreme environment; the raw materials of the microbial agent are cheap and easy to obtain, the microbial agent has the advantages of being high in repairing efficiency, low in environmental perturbation and the like, and uranium polluted water bodies can be effectively repaired. Through the application of the microbial agent disclosed by the invention, hexavalent uranium is directly mineralized into hexavalent uranium minerals which are large in particle diameter, small in influence by aerobic environment and capable of stably existing by a non-reduced mineralization method, the mineralization effect is better, the repairing effect is increased, the application range is extended, and the microbial agent is simple to operate and easy in large-scale production and has favorable application prospects.

Description

technical field [0001] The invention belongs to the field of bioremediation of radioactive pollution, in particular to a microbial agent for effectively treating uranium pollution and its application. Background technique [0002] At present, the nuclear energy industry is in a period of rapid development. However, a large amount of uranium-containing waste and uranium-containing wastewater will be produced in the process from uranium mining and smelting to enrichment. According to statistics, there are more than 2×10 9 Tons of uranium tailings are a long-term threat to human health. In addition, because phosphate fertilizers and potassium fertilizers also contain certain radioactive elements, the use of chemical fertilizers in agriculture will also bring certain uranium pollution. These discarded uranium will be enriched into the human body through the food chain. The radioactivity, high toxicity and carcinogenicity of hexavalent uranium will cause systemic diseases mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20G21F9/18C12R1/07C12R1/085C12R1/865
CPCC12N1/20G21F9/18
Inventor 林璋卫亚丽刘学明邓洪刘炜珍
Owner SOUTH CHINA UNIV OF TECH