High-purity EPA glyceride and DHA glyceride preparation method

Abstract

The invention discloses a high-purity EPA glyceride and DHA glyceride preparation method. The method includes steps: (1) performing fish oil enzyme catalytic hydrolysis, to be specific, adding water, fish oil and glyceryl ester hydrolase into a reactor, enabling reactants to be in an emulsified state under the condition of stirring, and adjusting pH, wherein final reaction products are fatty acid and glycerin aqueous solution; (2) separating and purifying fish oil fatty oil by a film evaporator and a rectifying tower to obtain high-purity EPA and DHA; (3) synthesizing EPA glyceride from EPA and glycerin through lipase; (4) synthesizing DHA glyceride from DHA and glycerin through lipase. The method is free of chemicals such as ethyl alcohol and urea, and a production process is simple; a multistage molecular distillation separation method which fails to realize large-scale production is substituted by a rectifying tower separation method which is easy to implement large-scale production, so that production efficiency is improved, and industrial environmental pollution is abated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of high-purity EPA glyceride and DHA glyceride. Background technique [0002] Omega-3 (Omega-3) is an important class of polyunsaturated fatty acids. Fish oil-derived EPA supplements can increase the concentration of EPA in the body. Arthritis) effective. In addition, EPA was also shown to improve the effectiveness of a drug for severe chronic psoriasis (a combination of low-dose etretinate and a corticosteroid). [0003] In the early 21st century, foreign medical clinical research found through a series of large-scale clinical studies that the purity of Omega-3 (Omega-3), the active ingredient in fish oil, determines the efficacy of fish oil products, that is, the purity of Omega-3 in fish oil is above 85%. have clinical significance. A clinical study published in the official journal "PLEFA" of the International Society for Fatty Acids and Lipids...

AI Technical Summary

Problems solved by technology

[0009] In recent years, enzymatic preparation of EPA glycerides and DHA glycerides has also received widespread attention, but at present, ethanol and fish oil are used for transesterification, and fatty acid ethyl esters are separated and purified by urea inclusion or molecular distillation, and then purified with high purity The fatty acid ethyl esters and glycerol are enzymatically transesterified to produce high-purity EPA glycerides and DHA glycerides. Therefore, the production plant must be equipped with an ethanol recycling system. Level (7 or more) molecular distillation can increase the EPA content to more than 90%, resulting in high energy consumption, many process equipment, high cost, low production efficiency, and difficulty in large-scale production

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