Silk fibroin hemostatic material and preparation method thereof

A silk fibroin and hemostatic material technology, applied in the field of biomaterials and biomedicine, can solve problems such as defects in rapid hemostatic performance, lack of tissue adhesion properties, and influence on hemostatic effect, and achieve excellent hemostatic performance and simple and convenient preparation process , the effect of maintaining biocompatibility

Inactive Publication Date: 2019-11-08
NANTONG TEXTILE & SILK IND TECH RES INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its degradation in vivo is poor, and its rapid hemostasis performance is defective.
For example, the patent application number CN201710088690.4, the invention patent titled "Silk Fibroin Hemostatic Material and Its Preparation Method" discloses that the silk fibroin solution and the polyethylene glycol solution are in a volume ratio of 10:0.25-10:3. Mixed with each other and freeze-dried to obtain a silk fibroin hemostatic material; the silk fibroin hemostatic material has good water absorption, is tightly combined with the wound and is not easy to fall off, but it is easy to mix the silk fibroin and polyethylene glycol solution during the preparation process. Completely induce silk fibroin to form a stable β-sheet structure, which does not have tissue adhesion properties and affects the hemostatic effect

Method used

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  • Silk fibroin hemostatic material and preparation method thereof
  • Silk fibroin hemostatic material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 silk fibroin solution

[0028] In 4L of boiled deionized water, add 3g of anhydrous NaHCO 3 and 1 g anhydrous Na 2 CO 3 , after fully dissolving, add 80g silkworm cocoon, adjust the state of the solution to slightly boil, stir once every 10min with a glass rod, wash it with deionized water after 30min, repeat the above operation 3 times; after fully washing, tear the silk and spread it Flatten and dry in an oven at 60°C. Take 100mL of 9.3mol / L lithium bromide solution and pour it into a conical flask, heat it to 65°C in a constant temperature magnetic stirrer, add 15g of degummed silk fibroin several times, dissolve it at 65±2°C for 30-40min, After fully cooling, put it into a dialysis bag and seal it for dialysis. Dialyze in deionized water at low temperature for 3 to 4 days. After the dialysis is completed, filter the obtained silk fibroin solution with absorbent cotton, put it into a reagent bottle, and store it at low temperature f...

Embodiment 2

[0029] The preparation of embodiment 2 silk fibroin hemostatic material

[0030] (1) Adjust the concentration of the pure silk fibroin solution in Example 1 to 15 g / L with deionized water to obtain an aqueous silk fibroin solution. Adding riboflavin to the silk fibroin aqueous solution, mixing evenly to obtain a mixed solution, the concentration of riboflavin in the mixed solution is 0.1 mM.

[0031] Under the irradiation of ultraviolet light, riboflavin undergoes energy transfer to generate active oxygen free radicals mainly composed of singlet oxygen. The active oxygen free radicals can react with various molecules and induce tyrosine residues in silk fibroin macromolecules. The base groups produce chemical crosslinks, thereby gelling.

[0032] (2) Use an oxygen generator to extract pure oxygen, continuously feed oxygen into the mixed solution for 15 minutes, and keep the partial pressure of oxygen in the mixed solution in a saturated state.

[0033](3) Pour the oxygen-sat...

Embodiment 3

[0042] The preparation of embodiment 3 silk fibroin hemostatic material

[0043] (1) Adjust the concentration of the pure silk fibroin solution in Example 1 to 15 g / L with deionized water to obtain an aqueous silk fibroin solution. Adding riboflavin to the silk fibroin aqueous solution, mixing evenly to obtain a mixed solution, the concentration of riboflavin in the mixed solution is 0.2mM.

[0044] (2) Use the oxygen generator to extract pure oxygen, continuously feed oxygen into the mixed liquid for 10 minutes, and keep the partial pressure of oxygen in the mixed liquid in a saturated state.

[0045] (3) Pour the oxygen-saturated mixed solution into a transparent mold such as a beaker, so that the mixed solution has a depth of 15mm, and put it into an ice bath to cool, and the temperature of the ice bath is 6°C.

[0046] (4) The mixed solution is irradiated with ultraviolet light in a low-temperature environment in an ice bath to obtain a cross-linked silk fibroin hydrogel....

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Abstract

The invention discloses a silk fibroin hemostatic material and a preparation method thereof. The preparation method comprises the following steps: adding photosensitizer riboflavin into a silk fibroinaqueous solution, and performing uniform mixing to obtain a mixed solution; continuously introducing oxygen into the mixed solution, and keeping oxygen tension in the mixed solution in a saturated state; pouring the oxygen-saturated mixed solution into a transparent die, and performing cooling; irradiating the mixed solution with ultraviolet light to obtain cross-linked silk fibroin hydrogel, andcontrolling the temperature of the mixed solution in the ultraviolet light irradiation process to be 0-8 DEG C; taking out the silk fibroin hydrogel from the die, and performing drying to obtain silkfibroin xerogel; and crushing the silk fibroin xerogel into powder to obtain the silk fibroin hemostatic material. Through cross-linking in a low-temperature environment, the preparation method can effectively reduce the reaction temperature, effectively limit the generation of a crystalline structure, and greatly improve the swelling capacity and the rapid blood suction performance of the material.

Description

technical field [0001] The invention relates to the technical fields of biological materials and biomedicine, in particular to a preparation method of a silk fibroin hemostatic material and the silk fibroin hemostatic material prepared by the preparation method. Background technique [0002] The development of efficient, fast, safe and economical hemostatic materials is a key difficulty that needs to be solved urgently in clinical operations and first aid. Existing medical hemostatic materials mainly include chitosan, fibrous collagen, fibrin glue, oxidized cellulose, calcium alginate cellulose, gelatin sponge and the like. However, these hemostatic materials have the disadvantages of low biocompatibility, complex synthesis, high price, slow hemostasis, and easy infection. The development of a material with good biocompatibility, low price and excellent hemostatic performance has great social benefits. [0003] Silk fibroin is a kind of natural fiber material, which has se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/108A61L24/0042A61L24/001A61L24/0036A61L2400/04C08L89/00
Inventor 卢神州姜福建温壮壮谭郭泓芳刘诗喆陈姣姣向华菊
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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